BBa_K1947023BBa_K1947023 Version 1 (Component)This part serves as a catch system expressed in <i>E. coli.
BBa_K1947025BBa_K1947025 Version 1 (Component)This part serves as a catch system expressed in AMB-1.
BBa_K1995025BBa_K1995025 Version 1 (Component)this part can bind heavy metal ions
BBa_K1947029BBa_K1947029 Version 1 (Component)This part is used to improve the protein purification.
BBa_K1932005BBa_K1932005 Version 1 (Component)This device is made up of BBa_K1932000;BBa_K1932001;BBa_K1932004
BBa_K1932006BBa_K1932006 Version 1 (Component)This device is constructed to express TAT-apoptin fused with sec2.
BBa_K1932007BBa_K1932007 Version 1 (Component)This device is constructed for the expression of TAT-apoptin fused with tmp1.
BBa_K2066032BBa_K2066032 Version 1 (Component)whatever this is
Orai1 V102BBa_K2028000 Version 1 (Component)This part is the cDNA of a mutated calcium ion channel protein.
GG98BBa_K2145123 Version 1 (Component)This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer
GG100BBa_K2145125 Version 1 (Component)This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer
BBa_M50011BBa_M50011 Version 1 (Component)This is an E. coli specific sensor for hydrogen peroxide.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Collection)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
.nt testnt_test_collection_collection Version 1 (Collection)i think this will break
BBa_K362007BBa_K362007 Version 1 (Component)This composite part has the promoter ,ahpC-promoter controlled by OxyR protein, and GFP - T-T
CapDBBa_K314970 Version 1 (Component)this is a really long short description. Will it still go through? this is a really long short descr
BBa_J70652BBa_J70652 Version 1 (Component)This tests adding an internal strep tag linker to a protein using J70590 as a te
BBa_J70653BBa_J70653 Version 1 (Component)This tests adding an internal strep tag linker to a protein using J70590 as a te
BBa_T1006BBa_T1006 Version 1 (Component)Kill this part
BBa_T1007BBa_T1007 Version 1 (Component)Kill this part
BBa_T1010BBa_T1010 Version 1 (Component)Kill this test
BBa_K758000BBa_K758000 Version 1 (Component)UAS , this is the enhancer . And this enhancer is used to identify the protein localization .
BBa_K758002BBa_K758002 Version 1 (Component)GAL4, this protein binds the UAS
BBa_K737000BBa_K737000 Version 1 (Component)We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig
BBa_K737001BBa_K737001 Version 1 (Component)We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig
BBa_K758003BBa_K758003 Version 1 (Component)UAS, this part consists of five UAS sequences and hsp70 TATA.
BBa_K758004BBa_K758004 Version 1 (Component)GAL4, this part coding protein binds and activates the UAS sequence
UAS-LacZBBa_K758005 Version 1 (Component)UAS LacZ, this part expresses LacZ in the presence of GAL4
UAS-EGFPBBa_K758006 Version 1 (Component)UAS EGFP, this part expresses EGFP in the presence of GAL4 protein.
AckABBa_K737002 Version 1 (Component)This coding is the acetate kinase.
BBa_K737003BBa_K737003 Version 1 (Component)This part is polyphosphate kinase.
BBa_K737016BBa_K737016 Version 1 (Component)This part conteins gvpA protein's coding sequence.
BBa_K737017BBa_K737017 Version 1 (Component)This part conteins gvpC-20psi protein
BBa_K737022BBa_K737022 Version 1 (Component)This part is gvpC-20 coding gene with GFP mut 3.1 fusion gene
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_T1001BBa_T1001 Version 1 (Component)Kill this part
BBa_K294000BBa_K294000 Version 1 (Component)This is the coding sequence for the heat shock protein hsp15 from E. coli
BBa_K294205BBa_K294205 Version 1 (Component)This is a coding sequence of heat shock protein from E.coli
BBa_K294030BBa_K294030 Version 1 (Component)This is the ribozyme for ligating two RNA
BBa_K233307BBa_K233307 Version 1 (Component)TorA- This part is a export tag that utilizes the Twin Arginine Transport pathway(TAT)
BBa_K233306BBa_K233306 Version 1 (Component)YcdB - This part is a export tag that utilizes the Twin Arginine Transport pathway(TAT)
BBa_K188006BBa_K188006 Version 1 (Component)Expression of ccdB for self-destruction of bacteria. Since promoter lux/cIIp22, this sequence can be
BBa_K195408BBa_K195408 Version 1 (Component)This part is composed of PelB,F10scFv and C-IgAP
BBa_K195402BBa_K195402 Version 1 (Component)This part is composed of RBS,Lpp-OmpA-linker and F10scFv
BBa_K195403BBa_K195403 Version 1 (Component)This part is composed of pTetR,RBS,Lpp-OmpA-linker and F10scFv
BBa_K195404BBa_K195404 Version 1 (Component)This part is composed of pTetR,RBS,Lpp-OmpA-linker,F10scFv and terminator
BBa_K195406BBa_K195406 Version 1 (Component)This part is composed of F10scFv and C-IgAP
BBa_K195407BBa_K195407 Version 1 (Component)This part is composed of PelB and F10scFv