pLac ColE2
BBa_K914001 Version 1 (Component)
pLac Colicin E2 Immunity protein

BBa_K914002
BBa_K914002 Version 1 (Component)
repressilator RBS Colicin E2 Immunity protein

BBa_K883160
BBa_K883160 Version 1 (Component)
CCMV_Negative coat protein

BBa_K883151
BBa_K883151 Version 1 (Component)
CCMV coat protein gene 25AA N-terminal replaced with HIStag under IPTG inducable promotor

gpS
BBa_K836006 Version 1 (Component)
Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K157010
BBa_K157010 Version 1 (Component)
glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac

linker
BBa_K157013 Version 1 (Component)
15 aa flexible glycine-serine protein domain linker; Freiburg standard

BBa_K156009
BBa_K156009 Version 1 (Component)
OFP (orange fluorescent protein)

SBFP2
BBa_K156010 Version 1 (Component)
SBFP2 (strongly enhanced blue fluorescent protein)

BBa_I0466
BBa_I0466 Version 1 (Component)
RhlR Protein Generator

BBa_K116001
BBa_K116001 Version 1 (Component)
nhaA promoter, that can be regulated by pH and nhaR protein.

BBa_K079051
BBa_K079051 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator

BBa_K079052
BBa_K079052 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato

BBa_K127013
BBa_K127013 Version 1 (Component)
gas vesicle protein A from cyanobacteria Anabaena

BBa_J36835
BBa_J36835 Version 1 (Component)
Lipoprotein signal peptide

BBa_J36836
BBa_J36836 Version 1 (Component)
Outer membrane protein A, aa 46-66

BBa_K116102
BBa_K116102 Version 1 (Component)
IPTG inducible membrane protein generator

BBa_K129003
BBa_K129003 Version 1 (Component)
LamB-maltose transporter membrane protein

BBa_J70030
BBa_J70030 Version 1 (Component)
A BioScaffold Part (Uses PpiI), Protein Tail Remover see Part Design Page

BBa_J70032
BBa_J70032 Version 1 (Component)
A Composite BioScaffold Part (PpiI and PsrI) for Protein Fusions (see Design Page)

BBa_J70033
BBa_J70033 Version 1 (Component)
A BioMortar Part for Creating GSGS Protein Fusions (see Design page)

BBa_J69516
BBa_J69516 Version 1 (Component)
lacI-regulated tetR invertor Protein Generator

BBa_J70060
BBa_J70060 Version 1 (Component)
BBa_J70010 (PpiI) protein fusion test part

BBa_J70070
BBa_J70070 Version 1 (Component)
BBa_J70012 (PsrI) protein fusion test part

BBa_J70080
BBa_J70080 Version 1 (Component)
BBa_J70030 (PpiI) protein fusion test part

BBa_J70090
BBa_J70090 Version 1 (Component)
BBa_J70032 (PpiI/PsrI) protein fusion test part

BBa_J70061
BBa_J70061 Version 1 (Component)
BBa_J70009 (PpiI) protein fusion test part

tetA(C)f
BBa_J31007 Version 1 (Component)
tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]

BBa_J70121
BBa_J70121 Version 1 (Component)
R0040 regulated expression of a lacZa.GFP fusion protein

BBa_M11006
BBa_M11006 Version 1 (Component)
ArsC arsenate reductase protein

BBa_M11011
BBa_M11011 Version 1 (Component)
Chloramphenicol Resistance Protein

BBa_M11080
BBa_M11080 Version 1 (Component)
silE (coding sequence for Silver binding protein)

BBa_M11081
BBa_M11081 Version 1 (Component)
cutA (coding sequence for copper binding protein)

BBa_M11076
BBa_M11076 Version 1 (Component)
NrtA: nitrate/nitrite binding protein coding region

BBa_M11036
BBa_M11036 Version 1 (Component)
D-galactose-binding periplasmic protein (D-galactose/ D-glucose-binding protein)

BBa_M11012
BBa_M11012 Version 1 (Component)
CheA Chemotaxis reception protein

BBa_M11078
BBa_M11078 Version 1 (Component)
OmpC + Copper-Binding Protein

BBa_M11083
BBa_M11083 Version 1 (Component)
MerP, coding sequence for mercuric ion binding protein

ompC w/Cad
BBa_M11084 Version 1 (Component)
E coli outer membrane protein C (ompC) with Cadmium Binding protien

BBa_M11052
BBa_M11052 Version 1 (Component)
Bacteriorhodopsin Related Protein

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o

BBa_M10041
BBa_M10041 Version 1 (Component)
{a~INP˃} | C-terminal displaying Ice Nucleation Protein

BBa_M10020
BBa_M10020 Version 1 (Component)
{˂TraT˃} C-terminal Display Protein