ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

BBa_K619898
BBa_K619898 Version 1 (Component)
medium length prfA-UTR thermosensor from Lysteria Monocytogenes

BBa_I712074
BBa_I712074 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

BBa_K619889
BBa_K619889 Version 1 (Component)
prfA-UTR, RNA thermosensor from Listeria monocytogenes

BBa_K833007
BBa_K833007 Version 1 (Component)
Mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K833005
BBa_K833005 Version 1 (Component)
Mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K833003
BBa_K833003 Version 1 (Component)
mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K833004
BBa_K833004 Version 1 (Component)
Mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K833010
BBa_K833010 Version 1 (Component)
Mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K833011
BBa_K833011 Version 1 (Component)
Mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K833009
BBa_K833009 Version 1 (Component)
Mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K619893
BBa_K619893 Version 1 (Component)
mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K833001
BBa_K833001 Version 1 (Component)
Mutated prfA-UTR thermosensor from Listeria monocytogenes

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

LacZ
BBa_I732005 Version 1 (Component)
lacZ (encoding beta-galactosidase, full-length)

BBa_K177026
BBa_K177026 Version 1 (Component)
listeriolysin O 399G->C - locus from Listeria monocytogenes

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

BBa_K619890
BBa_K619890 Version 1 (Component)
mutated prfA thermosensor from listeria

BBa_K619891
BBa_K619891 Version 1 (Component)
Mutated prfA thermosensor from listeria

BBa_K619892
BBa_K619892 Version 1 (Component)
mutated prfA thermosensor from listeria

BBa_K2066019
BBa_K2066019 Version 1 (Component)
UNS 3 Sequence, from Torella et al., 2013

BBa_K115003
BBa_K115003 Version 1 (Component)
RNA thermometer (PrfA)

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

BBa_K914003
BBa_K914003 Version 1 (Component)
L-rhamnose-inducible promoter (pRha)

BBa_K619897
BBa_K619897 Version 1 (Component)
shortened prfA-UTR thermosensor

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

c2 P22
BBa_C0053 Version 1 (Component)
c2 repressor from Salmonella phage P22 (+LVA)