BBa_K1051356
BBa_K1051356 Version 1 (Component)
K1051301(clb2 promoter) + K1051053(K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 term

BBa_K542012
BBa_K542012 Version 1 (Component)
Catechol 2,3-dioxygenase with a C-terminal Arg-tag and Double Terminator (no promoter/RBS)

BBa_M36914
BBa_M36914 Version 1 (Component)
Codes for the benzylalcohol acetyltransferase enzyme

BBa_J70434
BBa_J70434 Version 1 (Component)
R0010.J70428.E0020 or J70431.E0020

BBa_J70433
BBa_J70433 Version 1 (Component)
R0010.J70428.E0030 or J70431.E0030

BBa_J70425
BBa_J70425 Version 1 (Component)
R0010.J70399.E0020 or J70405.E0020

BBa_J70432
BBa_J70432 Version 1 (Component)
R0010.J70428.E0040 or J70431.E0040

BBa_J70424
BBa_J70424 Version 1 (Component)
R0010.J70399.E0030 or J70405.E0030

BBa_K648030
BBa_K648030 Version 1 (Component)
Or with RBS B0034 and Mcherry

BBa_J70423
BBa_J70423 Version 1 (Component)
R0010.J70399.E0040 or J70405.E0040

BBa_K1366101
BBa_K1366101 Version 1 (Component)
Genetic construct for lpp gene deletion (C1)

BBa_K619888
BBa_K619888 Version 1 (Component)
RNA Thermometer or thermosensor (30-37 Celsius)

BBa_K1363609
BBa_K1363609 Version 1 (Component)
gate of or of RNA logic gates

Digitalizer
Digitalizer_collection Version 1 (Collection)
A genetic device to digitalize gene expression into a sharp on/off signal.

BBa_I754001
BBa_I754001 Version 1 (Component)
ech protein generator for vanillin biosynthesis

BBa_I769106
BBa_I769106 Version 1 (Component)
ech protein generator for vanillin biosynthesis

BBa_J24820
BBa_J24820 Version 1 (Component)
Renilla luciferase reporter device for DLARAP

BBa_K2180012
BBa_K2180012 Version 1 (Component)
Promoter PlepA +FUR regulatory sequence + RBS + LacI-LVA + Terminator + Pigment + RBS + Operateur La

BBa_I763000
BBa_I763000 Version 1 (Component)
-- No description --

BBa_K185033
BBa_K185033 Version 1 (Component)
An inverter of a special lactose operon system based on J23110-rbs34-lacI-dter-plac-rbs31

BBa_K1884009
BBa_K1884009 Version 1 (Component)
PSAD Promoter+GalAD-CRY2+UAS+FNR(fumarate and nitrate reduction)+PSAD Terminator

BBa_K587012
BBa_K587012 Version 1 (Component)
iTast -> Antikey sequence for high concentration mechanism

BBa_K587008
BBa_K587008 Version 1 (Component)
Crxst -> Lock sequence for low concentration mechanism

BBa_K648108
BBa_K648108 Version 1 (Component)
RecA without restriction sites or recombinant activity

BBa_K357002
BBa_K357002 Version 1 (Component)
muconolactone Delta-isomerase or muconolactone D-isomerase

BBa_J70405
BBa_J70405 Version 1 (Component)
R0010.J70399 (Ribosome Replacer or Tag Adder)

BBa_M30002
BBa_M30002 Version 1 (Component)
-- No description --

BBa_M30004
BBa_M30004 Version 1 (Component)
-- No description --

BBa_K116500
BBa_K116500 Version 1 (Component)
OmpF promoter that is activated or repressesed by OmpR according to osmolarity.

BBa_M30005
BBa_M30005 Version 1 (Component)
-- No description --

BBa_M30008
BBa_M30008 Version 1 (Component)
-- No description --

BBa_M30007
BBa_M30007 Version 1 (Component)
-- No description --

BBa_M30010
BBa_M30010 Version 1 (Component)
-- No description --

BBa_I735000
BBa_I735000 Version 1 (Component)
ech gene coding sequence for feruoyl CoA hydratese

BBa_K292009
BBa_K292009 Version 1 (Component)
Stem cell factor (SCF) or c-kit-ligand

BBa_K1363610
BBa_K1363610 Version 1 (Component)
key-1-of-or of RNA logic gates

BBa_K415069
BBa_K415069 Version 1 (Component)
pLux-Lac:GFP:Term:Term:PluxR/cI-OR :mCherry:Term:PtetR:LuxR:Term:Fixed-pLux/cI:LuxI

cln2
BBa_K105014 Version 1 (Component)
cln2 PEST destabilization domain for rapid protein turnover

BBa_K415007
BBa_K415007 Version 1 (Component)
pLux/cI-OR : RBS-mCherry : Term : pLux/cI-OR : RBS-luxI : Term : p(tetR) : RBS-luxR : Term

BBa_J24818
BBa_J24818 Version 1 (Component)
dLaRAP firefly luciferase reporter device for promoters

BBa_J70404
BBa_J70404 Version 1 (Component)
R0010.E0021.J70394.E0031 or J70398.E0031

BBa_J70403
BBa_J70403 Version 1 (Component)
R0010.E0031.J70394.E0021 or J70397.E0021

BBa_M11409
BBa_M11409 Version 1 (Component)
Promoter of pglnBP2 gene in Synechocystis sp. PCC 6803.

BBa_K1778002
BBa_K1778002 Version 1 (Component)
TRE-CYC1TATA is a recombinant promoter, which is constructed in order to make the Tet-on system func

BBa_M50016
BBa_M50016 Version 1 (Component)
Promoter, RBS and GolS Activator for Gold Detection

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K338062
BBa_K338062 Version 1 (Component)
GFP Reporter Device, Heat-Shock or IPTG Induced

BBa_K327017
BBa_K327017 Version 1 (Component)
lux OR delta cI activated "off" part