BBa_K737038
BBa_K737038 Version 1 (Component)
galK::GFP generator ligated to sRNA device controlled by IPTG

BBa_K737039
BBa_K737039 Version 1 (Component)
galK::GFP generator ligated to sRNA device controlled by aTc

BBa_K737040
BBa_K737040 Version 1 (Component)
galK::GFP generator ligated to TetR generator and constitutive sRNA device

BBa_K737041
BBa_K737041 Version 1 (Component)
galK::GFP generator ligated to LacI generator and constitutive sRNA device

BBa_K737042
BBa_K737042 Version 1 (Component)
constitutive Spot42 generator ligated to TetR generator

BBa_K737043
BBa_K737043 Version 1 (Component)
constitutive Spot42 generator ligated to lacI generator

BBa_K737053
BBa_K737053 Version 1 (Component)
Plac-GFP Generator ligated to TetR Generator

BBa_K737054
BBa_K737054 Version 1 (Component)
Ptet-GFP Generator ligated to LacI Generator

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K102906
BBa_K102906 Version 1 (Component)
TA8 gate from synthetic algorithm v1.1

BBa_K102907
BBa_K102907 Version 1 (Component)
TA9 gate from synthetic algorithm v1.1

BBa_K102908
BBa_K102908 Version 1 (Component)
TA10 gate from synthetic algorithm v1.1

BBa_K102909
BBa_K102909 Version 1 (Component)
TA11 gate from synthetic algorithm v1.1

BBa_K102910
BBa_K102910 Version 1 (Component)
TA12 gate from synthetic algorithm v1.1

BBa_K102911
BBa_K102911 Version 1 (Component)
TA13 gate from synthetic algorithm v1.2

BBa_K102021
BBa_K102021 Version 1 (Component)
TA0 gate in output test harness

BBa_K102031
BBa_K102031 Version 1 (Component)
TA1 gate in output test harness

BBa_K102041
BBa_K102041 Version 1 (Component)
TA2 gate in output test harness

BBa_K102051
BBa_K102051 Version 1 (Component)
TA3 gate in output test harness

BBa_K102061
BBa_K102061 Version 1 (Component)
TA6 gate in output test harness

BBa_K102905
BBa_K102905 Version 1 (Component)
TA7 gate from synthetic algorithm v1.1

BBa_K102071
BBa_K102071 Version 1 (Component)
TA7 gate in output test harness

BBa_K102081
BBa_K102081 Version 1 (Component)
TA8 gate in output test harness

BBa_K102091
BBa_K102091 Version 1 (Component)
TA9 gate in output test harness

BBa_K102101
BBa_K102101 Version 1 (Component)
TA10 gate in output test harness

BBa_K102111
BBa_K102111 Version 1 (Component)
TA11 gate in output test harness

BBa_K102121
BBa_K102121 Version 1 (Component)
TA12 gate in output test harness

BBa_K102131
BBa_K102131 Version 1 (Component)
TA13 gate in output test harness

BBa_K102141
BBa_K102141 Version 1 (Component)
TA14 gate in output test harness

BBa_K233003
BBa_K233003 Version 1 (Component)
AND Gate 1

BBa_K233005
BBa_K233005 Version 1 (Component)
AND gate 2

BBa_K233006
BBa_K233006 Version 1 (Component)
AND Gate 3

BBa_K091223
BBa_K091223 Version 1 (Component)
AND Gate supporting construct

BBa_K235025
BBa_K235025 Version 1 (Component)
[K235022][K235000] (NAND gate, pAra and pLac input signal control, mCherry output signal)

BBa_K235026
BBa_K235026 Version 1 (Component)
[K235022][K235001] (NAND gate control test, pLac positive control)

BBa_K235027
BBa_K235027 Version 1 (Component)
[K235024][K235000] (NAND gate control test, arabinose positive control)

BBa_K235028
BBa_K235028 Version 1 (Component)
[K235024][K235001] (NAND gate control test, positive control)

BBa_K228823
BBa_K228823 Version 1 (Component)
AND GATE lacP+SupD+AraC+RBS(J44001)+T7ptag

BBa_K228824
BBa_K228824 Version 1 (Component)
AND GATE lacP+SupD+AraC+RBS(B0032)+T7ptag

BBa_K228825
BBa_K228825 Version 1 (Component)
AND GATE lacP+SupD+AraC+RBS(J61127)+T7ptag

BBa_K228826
BBa_K228826 Version 1 (Component)
AND GATE lacP+SupD+AraC+RBS(J61107)+T7ptag

BBa_K228827
BBa_K228827 Version 1 (Component)
AND GATE lacP+SupD+AraC+RBS(B0030)+T7ptag

BBa_K228828
BBa_K228828 Version 1 (Component)
AND GATE lacP+SupD+AraC+RBS(J61100)+T7ptag

BBa_K228829
BBa_K228829 Version 1 (Component)
AND GATE lacP+SupD+AraC+RBS(B0033)+T7ptag

BBa_K228830
BBa_K228830 Version 1 (Component)
AND GATE lacP+SupD+AraC+RBS(B0031)+T7ptag

BBa_K228831
BBa_K228831 Version 1 (Component)
AND GATE lacP+SupD+AraC+RBS(B0034)+T7ptag

BBa_K228255
BBa_K228255 Version 1 (Component)
AND GATE AraC+SupD+Sal+RBS(B0030)+T7ptag

BBa_K228256
BBa_K228256 Version 1 (Component)
AND GATE AraC+SupD+Sal+RBS(B0031)+T7ptag

BBa_K228257
BBa_K228257 Version 1 (Component)
AND GATE AraC+SupD+Sal+RBS(B0032)+T7ptag

BBa_K228258
BBa_K228258 Version 1 (Component)
AND GATE AraC+SupD+Sal+RBS(B0033)+T7ptag