atRS-RFP
BBa_K537008 Version 1 (Component)
Atrazine Riboswitch-mRFP

mRFP1-NLS
BBa_K678034 Version 1 (Component)
mRFP1-NLS, targeted to the nucleus

BBa_K568006
BBa_K568006 Version 1 (Component)
Intermediate synthetic part of optogenetical AND-Gate

BBa_K581000
BBa_K581000 Version 1 (Component)
sgrS1+Terminator (small RNA regulator, conjugate part of ptsG1)

CspB | RFP
BBa_K525234 Version 1 (Component)
Fusion Protein of mRFP, S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7, RBS

mdnA-myc-g
BBa_K627006 Version 1 (Component)
Fusion part of mdnA gene (from mdn-cluster) with myc-tag and gene III

AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease

AraC_14_3C
BBa_K627011 Version 1 (Component)
Fusion part of pBAD arabinose-inducible induction system and the HRV 14_3C protease

BBa_K560000
BBa_K560000 Version 1 (Component)
Blue Light Sensor+PtrpL+mRFP1

BBa_J22146
BBa_J22146 Version 1 (Component)
The Lac Y and mRFP gene under the RecA(SOS) promoter.

BBa_K581003
BBa_K581003 Version 1 (Component)
SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)

BBa_K581005
BBa_K581005 Version 1 (Component)
Pc+sgrS(wt)+Terminator (small RNA regulator, conjugate part of ptsG(wt))

BBa_K737000
BBa_K737000 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K737001
BBa_K737001 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K758003
BBa_K758003 Version 1 (Component)
UAS, this part consists of five UAS sequences and hsp70 TATA.

UAS-LacZ
BBa_K758005 Version 1 (Component)
UAS LacZ, this part expresses LacZ in the presence of GAL4

UAS-EGFP
BBa_K758006 Version 1 (Component)
UAS EGFP, this part expresses EGFP in the presence of GAL4 protein.

BBa_K739002
BBa_K739002 Version 1 (Component)
pRax-rbs-mRFP

BBa_K739003
BBa_K739003 Version 1 (Component)
promoter of raxRH + RBS + mRFP

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

mRFP1
BBa_I13502 Version 1 (Component)
Screening plasmid intermediate

BBa_J70031
BBa_J70031 Version 1 (Component)
Linker for BioScaffold Parts, example of Gamma BioScaffold part

BBa_K176007
BBa_K176007 Version 1 (Component)
pSB1A3 with the suffix of J61002 (mRFP)

BBa_J70342
BBa_J70342 Version 1 (Component)
J70315psri.f.1: J70315 psri forward part for pcr of S03621 (single stranded)

BBa_J70343
BBa_J70343 Version 1 (Component)
J70315psri.r.1: J70315 psri reverse part for pcr of S03621 (single stranded)

BBa_K239004
BBa_K239004 Version 1 (Component)
Part of narGP promoter, activated by Fnr during oxygen deficiency

BBa_K182001
BBa_K182001 Version 1 (Component)
Intermediate Part consisting of LacI ( RBS+, Term +) and a Tet operator.

BBa_K182002
BBa_K182002 Version 1 (Component)
Intermediate Part consisting of a cI regulated promoter and TetR (RBS+, Term-).

BBa_K223021
BBa_K223021 Version 1 (Component)
SoxS + Part:BBa_K152005 (Promoterless Synthetic Operon of CrtE,B,I,Y and GFP)

BBa_K182100
BBa_K182100 Version 1 (Component)
Intermediate Part consisting of Lambda cI with Reporter Gene and double Terminators

mRFPr
BBa_J31008 Version 1 (Component)
RFP reverse

BBa_K182003
BBa_K182003 Version 1 (Component)
Intermediate part consisting of CI promoter followed by tetR, LacI and Tet Operator

BBa_K182103
BBa_K182103 Version 1 (Component)
Composite part of K182100 and K182102

BBa_K177033
BBa_K177033 Version 1 (Component)
pcI lam RBS.3 LacI pcl lam RBS mRFP1 terminator

BBa_K200005
BBa_K200005 Version 1 (Component)
OtsA: Part 1 of 2 for trehalose producing enzymes.

BBa_K200006
BBa_K200006 Version 1 (Component)
OtsB: Part 2 of 2 coding for trehalose producing enzymes

mRFP1
BBa_E2050 Version 1 (Component)
derivative of mRFP1, yeast-optimized

BBa_K180006
BBa_K180006 Version 1 (Component)
Game of Life - Primary plasmid (part 2) [lux pR, GFP and LacI generator]

FecI
BBa_K257017 Version 1 (Component)
Fec I, part of the signal transduction pathway FecARI

BBa_K247004
BBa_K247004 Version 1 (Component)
plux-rbs-mRFP

BBa_K272998
BBa_K272998 Version 1 (Component)
Constitutive expression cassette for TetR repressor and mRFP 1

BBa_K272500
BBa_K272500 Version 1 (Component)
Constitutive expression cassette for TetR repressor-mRFP-Spectinomycin resistance marker

BBa_K272990
BBa_K272990 Version 1 (Component)
Rbs-Tet repressor-mRFP

BBa_K272502
BBa_K272502 Version 1 (Component)
Constitutive expression cassette for TetR repressor and mRFP 1-terminator

BBa_K195408
BBa_K195408 Version 1 (Component)
This part is composed of PelB,F10scFv and C-IgAP

BBa_K195402
BBa_K195402 Version 1 (Component)
This part is composed of RBS,Lpp-OmpA-linker and F10scFv

BBa_K195403
BBa_K195403 Version 1 (Component)
This part is composed of pTetR,RBS,Lpp-OmpA-linker and F10scFv