Free Genes Feature Librariesfree_genes_feature_libraries_collection Version 1 (Unknown)A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
iGEM Feature Librariesigem_feature_libraries_collection Version 1 (Unknown)A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
BBa_B0012BBa_B0012 Version 1 (Component)TE from coliphageT7
BBa_B0010BBa_B0010 Version 1 (Component)T1 from E. coli rrnB
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
GFPBBa_E0040 Version 1 (Component)green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
BBa_B0030BBa_B0030 Version 1 (Component)RBS.1 (strong) -- modified from R. Weiss
tetRBBa_C0040 Version 1 (Component)tetracycline repressor from transposon Tn10 (+LVA)
lacIBBa_C0012 Version 1 (Component)lacI repressor from E. coli (+LVA)
lasrBBa_C0079 Version 1 (Component)lasR activator from P. aeruginosa PAO1(+LVA)
lasIBBa_C0078 Version 1 (Component)autoinducer synthetase for PAI from Pseudomonas aeruginosa
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
ATGATG Version 1 (Component) lasRBBa_C0179 Version 1 (Component)lasR activator from P. aeruginosa PAO1(no LVA)
mRFP1BBa_E1010 Version 1 (Component)**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
BBa_K844000BBa_K844000 Version 1 (Component)10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
cI lamBBa_C0051 Version 1 (Component)cI repressor from E. coli phage lambda (+LVA)
BBa_K855004BBa_K855004 Version 1 (Component)pvdQ gene form pseudomonas aeruginosa PAO1 with his tag added before stop condon
RGD-His-StBBa_K133036 Version 1 (Component)RGD-His affinity tag-Stop
His-StopBBa_K133133 Version 1 (Component)His affinity tag-stop
eyfpBBa_E0030 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
lasIBBa_C0178 Version 1 (Component)autoinducer synthetase for PAI from Pseudomonas aeruginosa (no LVA)
luxIBBa_C0061 Version 1 (Component)autoinducer synthetase for AHL
BBa_J04500BBa_J04500 Version 1 (Component)IPTG inducible promoter with RBS
Scar25 ATGBBa_K1319106 Version 1 (Component)RFC 25 Translation Start Scar Sequence
hixCBBa_J44000 Version 1 (Component)hixC binding site for Salmonella typhimurium Hin recombinase
GerEBO_11469 Version 1 (Component) rhlRBBa_C0071 Version 1 (Component)rhlR repressor/activator from P. aeruginosa PA3477 (+LVA)
GC Optimised Sequence PullGC_Optimised Version 1 (Activity)Used to indicate that a sequence was GC optimised after pulling from the source and before submission
BBa_K1051003BBa_K1051003 Version 1 (Component)Stop codon free GFP in RFC[23] standard
rhIRBBa_C0171 Version 1 (Component)rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)
YFPBBa_E0032 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
BBa_K1051000BBa_K1051000 Version 1 (Component)Stop codon free RFP in RFC[23] standard
ecfpBBa_E0020 Version 1 (Component)engineered cyan fluorescent protein derived from A. victoria GFP
RFC105 ZBBa_K1362421 Version 1 (Component)C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z
norCBBa_K1356004 Version 1 (Component)Nitric oxide reductase (norC) from Pseudomonas aeruginosa PAO1
PA1_04sPA1_04s Version 1 (Component) BBa_J04031BBa_J04031 Version 1 (Component)GFP w/LVA tag
nosZBBa_K1356006 Version 1 (Component)Nitrous oxide reductase (nosZ) from Pseudomonas aeruginosa PAO1
dCas 9BBa_K1051800 Version 1 (Component)protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.