BBa_K283017
BBa_K283017 Version 1 (Component)
prosseA-rbs-T7 RNAP (Backbone: pSB1A2)

BBa_K283027
BBa_K283027 Version 1 (Component)
rbs-GFP(LVA)-terminators (Backbone: pSB1AK3)

BBa_K283045
BBa_K283045 Version 1 (Component)
prosseA-rbs- T7 RNAP (Backbone: pSB3C5)

BBa_K1443003
BBa_K1443003 Version 1 (Component)
RFP Binding Forward Primer for Backbone Amplification

BBa_K1443004
BBa_K1443004 Version 1 (Component)
RFP Binding Reverse Primer for Backbone Amplification

BBa_K797016
BBa_K797016 Version 1 (Component)
Plasmid Backbone including M-13 primer region

BBa_K1560001
BBa_K1560001 Version 1 (Component)
pSCKiKo Knock-In Knock-Out Vector Backbone

pSB1A3 Ara
BBa_K627014 Version 1 (Component)
Plasmid backbone pSB1A3 carrying the arabinose induction system

pSB4K50
BBa_K1362097 Version 1 (Component)
Low copy BioBrick expression backbone carrying Kan resistance

BBa_K731702
BBa_K731702 Version 1 (Component)
BBa_K731700 backbone with BBa_K731722 E. coli terminator

pSB1A3 lac
BBa_K627015 Version 1 (Component)
Plasmid backbone pSB1A3 carrying the lac operon induction system

BBa_K1362092
BBa_K1362092 Version 1 (Component)
pSB1C30: High copy BioBrick cloning/expression backbone carrying Cm resitance

BBa_K1362093
BBa_K1362093 Version 1 (Component)
pSB1K30: High copy BioBrick cloning/expression backbone carrying Kan resitance

pSB1T30
BBa_K1362094 Version 1 (Component)
pSB1T30: High copy BioBrick cloning/expression backbone carrying Tet resitance

BBa_K1178001
BBa_K1178001 Version 1 (Component)
Plasmid Backbone pSB1C95 for RFC 95 Open Sequence Initiative

pSB4A50
BBa_K1362095 Version 1 (Component)
pSB4A50: Low copy BioBrick cloning/expression backbone carrying Amp resistance

pSB4C50
BBa_K1362096 Version 1 (Component)
pSB4C50: Low(?) copy BioBrick cloning/expression backbone carrying Cm resistance

Prefix-R
BBa_G1001 Version 1 (Component)
Reverse primer for amplifying BioBrick plasmid backbones by PCR (Prefix-r)

Suffix-F
BBa_G1000 Version 1 (Component)
Forward primer for amplifying BioBrick plasmid backbones by PCR (Suffix-f)

Prefix-R
BBa_G1003 Version 1 (Component)
Reverse primer for amplifying BioBrick plasmid backbones by PCR (Prefix-r)

Suffix-F
BBa_G1002 Version 1 (Component)
Forward primer for amplifying BioBrick plasmid backbones by PCR (Suffix-f)

BBa_K1065201
BBa_K1065201 Version 1 (Component)
Pxyl+GFP in an integrative <i>Bacillus subtilis</i> specific backbone

BBa_K2066118
BBa_K2066118 Version 1 (Component)
Synthetic Enhancer with 3X TetO cassette (52s) on UNS backbone

BBa_K2066113
BBa_K2066113 Version 1 (Component)
Synthetic Enhancer with 2X TetO cassette (55as) on UNS backbone

BBa_K368010
BBa_K368010 Version 1 (Component)
CMV-Promoter + TEV-Protease+p14*+TEV recogn. site

pSBBs2E
BBa_K823027 Version 1 (Component)
pSB_Bs2E: Empty backbone for integration into Bacillus subtilis lacA locus

BBa_K547000
BBa_K547000 Version 1 (Component)
ready-to-inject backbone for T3SS, SlrP taged, with Bsa I cloning site

BBa_K1961002
BBa_K1961002 Version 1 (Component)
RecO protein-SOS mediator protein

BBa_K1179006
BBa_K1179006 Version 1 (Component)
A backbone for ligation of the Acyl-TyA domain to a protein of your choice

BBa_K1961009
BBa_K1961009 Version 1 (Component)
RecO protein generator

pSBBs4S
BBa_K823022 Version 1 (Component)
pSB_Bs4S: Empty backbone for integration into <i/>Bacillus subtilis thrC</i> locus

BBa_K1085014
BBa_K1085014 Version 1 (Component)
pSB1AC3-HySp: Integrational backbone into <i>B. subtilis amyE</i> locus with IPTG inducible promoter

BBa_K650006
BBa_K650006 Version 1 (Component)
D. rad RecO (DR_0819)

BBa_K368007
BBa_K368007 Version 1 (Component)
tet-on Promoter (prev TRE) + TEV recogn. site+ N-degron+SF3b155

pSBBs1C
BBa_K823023 Version 1 (Component)
pSB_Bs1C: Empty backbone for integration into <i>Bacillus subtilis</i> <i>amyE</i> locus

BBa_K368012
BBa_K368012 Version 1 (Component)
CMV-promoter+TEV-protease+p14*+TEV recogn. site+eGFP+SV40PA

BBa_K929300
BBa_K929300 Version 1 (Component)
Potsdam Standard Backbone

BBa_K1698003
BBa_K1698003 Version 1 (Component)
BaseHunter : Activated to become detector for 32bp target sequence on SRY Gene

BBa_K1698004
BBa_K1698004 Version 1 (Component)
BaseHunter : Activated to become detector for 24bp target sequence on Mycobacterium genomes

BBa_K1698002
BBa_K1698002 Version 1 (Component)
BaseHunter : Activated to become detector for 62bp target sequence on SRY gene

BBa_K1698001
BBa_K1698001 Version 1 (Component)
BaseHunter : Human Papiloma Virus (HPV) Detector Part - Can be activated to become detector for 55bp

BBa_K283026
BBa_K283026 Version 1 (Component)
rbs-GFP(LVA)-terminators (Backbone: pSB1A2)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.