BBa_K1641009
BBa_K1641009 Version 1 (Component)
Fusion protein of Vika-EGFP-ssra, with RBS at beginning

BBa_K648101
BBa_K648101 Version 1 (Component)
RecA (mutated from RecA1 at amino acid 160 G-->A

BBa_I733007
BBa_I733007 Version 1 (Component)
Weight cells either turn blue or die in response to both inputs and HSL level

BBa_I3420
BBa_I3420 Version 1 (Component)
Test: Switch functionality and bistability (I3410.E0422.I3411.E0432.I3100.I3400.I3101.I3400.I3102.I3

BBa_K175035
BBa_K175035 Version 1 (Component)
Constitutive expression of GFP with medium RBS lock and inducible production of key for the lock

BBa_K175034
BBa_K175034 Version 1 (Component)
Constitutive expression of GFP with weak RBS lock and inducible production of key for the lock

BBa_M41008
BBa_M41008 Version 1 (Component)
AND Gate Promoter

(L-C)3
BBa_K365014 Version 1 (Component)
ClpX trimer with built-in linker at N-ter end

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.

BBa_K887004
BBa_K887004 Version 1 (Component)
Plac+alsS+ilvC+ilvD(each preceded by own zinc-finger and RBS)+Ptet+B0032+kivD+B0015

BBa_M45689
BBa_M45689 Version 1 (Component)
Constitutive promoter with a retinoic acid response element at the end

BBa_K1332005
BBa_K1332005 Version 1 (Component)
The 3' side of the intron(+exon fragment) from td gene of T4 phage

BBa_M45111
BBa_M45111 Version 1 (Component)
RBS and Phasin

BBa_I13035
BBa_I13035 Version 1 (Component)
3OC₆HSL Receiver Device with Inducible Control of LuxR and a YFP Output device

BBa_I716015
BBa_I716015 Version 1 (Component)
RFP without start ATG

BBa_K289009
BBa_K289009 Version 1 (Component)
pGADT7 is the AD Vector included with MATCHMAKER Two-Hybrid System 3.

BBa_J119316
BBa_J119316 Version 1 (Component)
Scaffold 2.0 for J-GGA (With the promoter between Junction A & B and the RBS in the junction B & C)

BBa_J70655
BBa_J70655 Version 1 (Component)
RFP optimized for expression in E. coli and M. florum

BBa_K806003
BBa_K806003 Version 1 (Component)
SeqA regulation of chromosome replication by preventing re-initiation at newly replicated origins

BBa_K861012
BBa_K861012 Version 1 (Component)
FadD and FadL with a RBS and a terminator

BBa_K323075
BBa_K323075 Version 1 (Component)
ATG cYFP link HIVC

BBa_K1088053
BBa_K1088053 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_M45136
BBa_M45136 Version 1 (Component)
ChrR30, Chromate and Uranium Reductase

BBa_K855006
BBa_K855006 Version 1 (Component)
pvdQ gene with a silent mutation at 1494 bp to remove the internal PstI site

BBa_K855005
BBa_K855005 Version 1 (Component)
pvdQ gene with a silent mutation at 1491 bp to remove the internal PstI site

BBa_K299800
BBa_K299800 Version 1 (Component)
standard biobrick scar (if next part starts with ATG)

BBa_K1332004
BBa_K1332004 Version 1 (Component)
The 5?? side of the intron(+exon fragment) from td gene of T4 phage

BBa_K202004
BBa_K202004 Version 1 (Component)
Hybrid promoter having multiple operator sites. Promoter has tetO2 with mutation at position 3

BBa_K1088059
BBa_K1088059 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_K1088052
BBa_K1088052 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

IodoY RS
BBa_K1416001 Version 1 (Component)
The tRNA synthetase/tRNA needed for incorporating 3-iodo-L-tyrosine (IodoY) at a UAG codon

BBa_K1361005
BBa_K1361005 Version 1 (Component)
CsgE, CsgF, CsgG, the outer membrane secrete device for curli fiber, at relatively low constitutive

BBa_J92001
BBa_J92001 Version 1 (Component)
Lead Remover and Reporter Device

BBa_J107021
BBa_J107021 Version 1 (Component)
aTc sensor (J23106 promoter) with GFP

BBa_K1484005
BBa_K1484005 Version 1 (Component)
AmilCP chromoprotein in BBa and MoClo standard

BBa_K1113001
BBa_K1113001 Version 1 (Component)
pSB4K5 with a promoter and RBS

BBa_K1463771
BBa_K1463771 Version 1 (Component)
MotA and MotB under J23112 promoter

BBa_K546547
BBa_K546547 Version 1 (Component)
Constitutive (tetR repressible) LacI and RFP expression

RBS-TetR-T
BBa_K079007 Version 1 (Component)
TetR protein with terminator and RBS

BBa_K1332003
BBa_K1332003 Version 1 (Component)
The 5?? side of the intron(+exon fragment) from td gene of T4 phage without stop codon

BBa_M31909
BBa_M31909 Version 1 (Component)
Part of Gene 3 and the BamHI Restriction Site

BBa_K2097001
BBa_K2097001 Version 1 (Component)
Blue chromoprotein with strong promoter and RBS

BBa_K1685002
BBa_K1685002 Version 1 (Component)
aeBlue with LVA tag and double terminator

BBa_J14462
BBa_J14462 Version 1 (Component)
Composite part comprised of J13002 and J04650

BBa_K1172506
BBa_K1172506 Version 1 (Component)
oprF with strong Promoter and strong RBS

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K079051
BBa_K079051 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator