Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
BBa_B0012BBa_B0012 Version 1 (Component)TE from coliphageT7
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
BBa_B0010BBa_B0010 Version 1 (Component)T1 from E. coli rrnB
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
GFPBBa_E0040 Version 1 (Component)green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
BBa_B0030BBa_B0030 Version 1 (Component)RBS.1 (strong) -- modified from R. Weiss
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
tetRBBa_C0040 Version 1 (Component)tetracycline repressor from transposon Tn10 (+LVA)
lacIBBa_C0012 Version 1 (Component)lacI repressor from E. coli (+LVA)
mRFP1BBa_E1010 Version 1 (Component)**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
cI lamBBa_C0051 Version 1 (Component)cI repressor from E. coli phage lambda (+LVA)
eyfpBBa_E0030 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
BBa_K1162004BBa_K1162004 Version 1 (Component)Cg-Defh1 antimicrobial peptide from the Pacific oyster (<i>Crassostrea gigas</i>)
YFPBBa_E0032 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
ecfpBBa_E0020 Version 1 (Component)engineered cyan fluorescent protein derived from A. victoria GFP
BBa_I712074BBa_I712074 Version 1 (Component)T7 promoter (strong promoter from T7 bacteriophage)
ECFPBBa_E0022 Version 1 (Component)enhanced cyan fluorescent protein derived from A. victoria GFP
BBa_K2066018BBa_K2066018 Version 1 (Component)UNS 2 Sequence, from Torella et al., 2013
BBa_B0016BBa_B0016 Version 1 (Component)Terminator (T7 RNAP specific, T_Phi)
phlFBBa_K1725040 Version 1 (Component)<i>phlF</i> encoding PhlF repressor from Pseudomonas protegens Pf-5
p_qrr4BBa_K131017 Version 1 (Component)p_qrr4 from <I>Vibrio harveyi</I>
aiiABBa_C0060 Version 1 (Component)autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone
BBa_K1315001BBa_K1315001 Version 1 (Component)The <i>pqsA</i> promoter from <i>Pseudomonas</i>
BBa_K1315008BBa_K1315008 Version 1 (Component)The <i>cblD</i> promoter from <i>Burkholderia</i>
BBa_K1159303BBa_K1159303 Version 1 (Component)Signal Peptide of SERK Receptor from <i>Physcomitrella patens</i> in RFC[25]
BBa_K1315005BBa_K1315005 Version 1 (Component)The <i>clp</i> gene from <i>Xanthomonas campestris</i>
BBa_K1315003BBa_K1315003 Version 1 (Component)The <i>rpfG</i> gene from <i>Xanthomonas campestris</i>
cI 434BBa_C0052 Version 1 (Component)cI repressor from phage 434 (+LVA)
BBa_K1315006BBa_K1315006 Version 1 (Component)The <i>manA</i> promoter from <i>Xanthomonas campestris</i>
CD4 signalBBa_I712009 Version 1 (Component)CD4 signal peptide; localizes to plasma membrane
BBa_K1162006BBa_K1162006 Version 1 (Component)LL-37 antimicrobial peptide from Humans (<i>Homo sapiens</i>)
BBa_K1315007BBa_K1315007 Version 1 (Component)The BCAM0228 Response Regulator from <i>Burkholderia</i>
BBa_K733010BBa_K733010 Version 1 (Component)<i>Ptms</i>+RBS+<i>ydcD</i>.
BBa_K531006BBa_K531006 Version 1 (Component)Optimized esp gene for <i>C. crescentus</i>
BBa_K733013BBa_K733013 Version 1 (Component)<i>Pveg</i>+R.B.S.
BBa_K733006BBa_K733006 Version 1 (Component)<i>ydjM</i>+<i>Bmp2</i>
BBa_K733001BBa_K733001 Version 1 (Component)<i>Ptms</i> - a constitutive promoter in <i>E. coli</i> and <i>B. subtilis</i>.
BBa_K1638003BBa_K1638003 Version 1 (Component)T25 domain of <i>cyaA</i> from <i>Bordetella pertussis</i> (IPTG inducible)