Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

p(tetR)
BBa_R0040 Version 1 (Component)
TetR repressible promoter

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

LacI
BBa_R0010 Version 1 (Component)
promoter (lacI regulated)

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

lacI+pL
BBa_R0011 Version 1 (Component)
Promoter (lacI regulated, lambda pL hybrid)

lux pR
BBa_R0062 Version 1 (Component)
Promoter (luxR & HSL regulated -- lux pR)

cI lam
BBa_R0051 Version 1 (Component)
promoter (lambda cI regulated)

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette

P(Lac) IQ
BBa_I14032 Version 1 (Component)
promoter P(Lac) IQ

LasR+PAI
BBa_R0079 Version 1 (Component)
Promoter (LasR & PAI regulated)

tetR
BBa_Q04400 Version 1 (Component)
QPI (B0034.C0040.B0015.R0040)

cI+luxR
BBa_R0065 Version 1 (Component)
Promoter (lambda cI and luxR regulated -- hybrid)

BBa_K864400
BBa_K864400 Version 1 (Component)
Ptac, trp & lac regulated promoter

lux pL
BBa_R0063 Version 1 (Component)
Promoter (luxR & HSL regulated -- lux pL)<br>

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

AraC
BBa_R0080 Version 1 (Component)
Promoter (AraC regulated)

cII p22
BBa_R0053 Version 1 (Component)
Promoter (p22 cII regulated)

RhlR+C4
BBa_R0071 Version 1 (Component)
Promoter (RhlR & C4-HSL regulated)

lasR
BBa_C0179 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(no LVA)

BBa_I13306
BBa_I13306 Version 1 (Component)
R0040.I13279 Tet-Regulated Las Amplifier

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

BBa_I13308
BBa_I13308 Version 1 (Component)
R0040.I13277 Tet-Regulated Cin Amplifier

cI 434
BBa_R0052 Version 1 (Component)
Promoter (434 cI regulated)

BBa_P0440
BBa_P0440 Version 1 (Component)
PoPS -> TetR [S0151]

BBa_I13305
BBa_I13305 Version 1 (Component)
R0011.I13279 Lac-Regulated Las Amplifier

BBa_I13304
BBa_I13304 Version 1 (Component)
R0040.I13271 Tet-Regulated Rhl Amplifier

BBa_A340620
BBa_A340620 Version 1 (Component)
Quadpart Amplifier (B0034.C0062.B0015.R0062)

Las CIO
BBa_I14016 Version 1 (Component)
P(Las) CIO

BBa_I13307
BBa_I13307 Version 1 (Component)
R0011.I13277 Lac-Regulated Cin Amplifier

BBa_I13303
BBa_I13303 Version 1 (Component)
R0011.I13271 Lac-Regulated Rhl Amplifier

cI lam
BBa_R1051 Version 1 (Component)
Promoter, Standard (lambda cI regulated)<br>