Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Collection)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

PfadR
BBa_K861060 Version 1 (Component)
PfadR, synthetic promoter with tandem FadR binding site

Pcar
BBa_K861171 Version 1 (Component)
Pcar, synthetic promoter repressed by CRP

BBa_K1699003
BBa_K1699003 Version 1 (Component)
gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm

BBa_K1699005
BBa_K1699005 Version 1 (Component)
gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm under U6 promoter

BBa_K1678004
BBa_K1678004 Version 1 (Component)
p48 synthetic promoter for L.plantarum

BBa_K1678002
BBa_K1678002 Version 1 (Component)
RibT promoted by p25 synthetic promoter

BBa_K1678003
BBa_K1678003 Version 1 (Component)
RibT promoted by p48 synthetic promoter

BBa_K2123204
BBa_K2123204 Version 1 (Component)
Bioaccumulator device: Strong promoter + OmpA fused to Synthetic Phytochelatin + B0015

BBa_K2136013
BBa_K2136013 Version 1 (Component)
hsp70 + rbsc2 synthetic constitutive promoter for Chlamydomonas

BBa_K2066126
BBa_K2066126 Version 1 (Component)
Synthetic Enhancer Project: NRII2302 Upstream Regulatory Region with Promoter

BBa_K2066544
BBa_K2066544 Version 1 (Component)
Synthetic Enhancer Project: glnAp2 promoter

BBa_K2066547
BBa_K2066547 Version 1 (Component)
Synthetic Enhancer Project: Spacer between Promoter and RBS

BBa_K1913022
BBa_K1913022 Version 1 (Component)
Synthetic plac-FixK2 hybrid promoter with RBS

BBa_K1913023
BBa_K1913023 Version 1 (Component)
Synthetic plac-FixK2 hybrid promoter with RBS

BBa_K1913024
BBa_K1913024 Version 1 (Component)
Synthetic ptet-FixK2 hybrid promoter with RBS

BBa_K1913029
BBa_K1913029 Version 1 (Component)
Synthetic plac-FixK2 hybrid promoter +RBS with mRFP

BBa_K1913030
BBa_K1913030 Version 1 (Component)
Synthetic plac-FixK2 hybrid promoter+RBS with mRFP

BBa_K1913031
BBa_K1913031 Version 1 (Component)
Synthetic ptet-FixK2 hybrid promoter+RBS with mRFP

BBa_K2066112
BBa_K2066112 Version 1 (Component)
Synthetic Enhancer Project: Ntr promoter driven NRII2302 (mut) on UNS Standard

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Collection)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

BBa_K515100
BBa_K515100 Version 1 (Component)
IAA biosynthetic genes under control of the Pveg2 promoter

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K152004
BBa_K152004 Version 1 (Component)
Promoterless Synthetic Operon of CrtE,B,I,Y

BBa_K152005
BBa_K152005 Version 1 (Component)
Promoterless Synthetic Operon of CrtE,B,I,Y and GFP

BBa_K223021
BBa_K223021 Version 1 (Component)
SoxS + Part:BBa_K152005 (Promoterless Synthetic Operon of CrtE,B,I,Y and GFP)

BBa_K203120
BBa_K203120 Version 1 (Component)
Synthetic promoter; HB17

BBa_K203122
BBa_K203122 Version 1 (Component)
Synthetic promoter; HB19

BBa_K203123
BBa_K203123 Version 1 (Component)
Synthetic promoter; HB1

BBa_K203127
BBa_K203127 Version 1 (Component)
Synthetic promoter; HB3

BBa_K203128
BBa_K203128 Version 1 (Component)
Synthetic promoter; HB20

BBa_K203129
BBa_K203129 Version 1 (Component)
Synthetic promoter HB25

BBa_K203130
BBa_K203130 Version 1 (Component)
Synthetic promoter; HB7

BBa_K203131
BBa_K203131 Version 1 (Component)
Synthetic promoter; HB24

BBa_K203132
BBa_K203132 Version 1 (Component)
Synthetic promoter; HB21

BBa_K203133
BBa_K203133 Version 1 (Component)
Synthetic promoter;HB23

BBa_K203134
BBa_K203134 Version 1 (Component)
Synthetic promoter; HB22

BBa_K203135
BBa_K203135 Version 1 (Component)
Synthetic promoter; HB11