ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

TAL
BBa_K1033000 Version 1 (Component)
Tyrosine ammonia-lyase (TAL) with RBS

BBa_K2066019
BBa_K2066019 Version 1 (Component)
UNS 3 Sequence, from Torella et al., 2013

TAL
BBa_K1129000 Version 1 (Component)
Tyrosine Ammonia Lyase (TAL) from Rhodobacter sphaeroides

BBa_I712074
BBa_I712074 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

aspA
BBa_C0083 Version 1 (Component)
aspartate ammonia-lyase

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

sam8
BBa_I742142 Version 1 (Component)
sam8 (tyrosine-ammonia lyase) coding sequence

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

BBa_K122005
BBa_K122005 Version 1 (Component)
Tyrosine Ammonia Lyase

BBa_J95021
BBa_J95021 Version 1 (Component)
RBS for Rhodobacter sphaeroides

rbs+sam8
BBa_I742148 Version 1 (Component)
sam8 (tyrosine ammonia lyase) with native rbs

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

BBa_J95023
BBa_J95023 Version 1 (Component)
promoter designed for Rhodobacter sphaeroides

BBa_K1129003
BBa_K1129003 Version 1 (Component)
Phenylalanine Ammonia Lyase (Enc-P) from Streptomyces maritimus

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

c2 P22
BBa_C0053 Version 1 (Component)
c2 repressor from Salmonella phage P22 (+LVA)

Plac-sam8
BBa_I742144 Version 1 (Component)
Plac+lacZ+sam8 (tyrosine ammonia lyase)

BBa_I15008
BBa_I15008 Version 1 (Component)
heme oxygenase (ho1) from Synechocystis

BBa_K801091
BBa_K801091 Version 1 (Component)
phenylalanine ammonia lyase (PAL) coding region

BBa_I15009
BBa_I15009 Version 1 (Component)
phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

sam8sam5
BBa_I742143 Version 1 (Component)
sam8+sam5 (tyrosine ammonia lyase + coumarate hydroxylase)

Y taRNA
BBa_K606034 Version 1 (Component)
Tyrosine amber supressor tRNA

BBa_J04031
BBa_J04031 Version 1 (Component)
GFP w/LVA tag

tADH1
BBa_J63002 Version 1 (Component)
ADH1 terminator from S. cerevisiae

BBa_K1751000
BBa_K1751000 Version 1 (Component)
RgPAL1：phenylalanine ammonia lyase (PAL) coding region

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

lasR
BBa_C0179 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(no LVA)

BBa_I712019
BBa_I712019 Version 1 (Component)
Firefly luciferase - luciferase from Photinus pyralis