Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

OmpR
BBa_R0082 Version 1 (Component)
Promoter (OmpR, positive)

BBa_K1906020
BBa_K1906020 Version 1 (Component)
ribothermometer modified A1 to work as positive cpntrol

OmpR
BBa_R0084 Version 1 (Component)
Promoter (OmpR, positive)

Prm +
BBa_I12007 Version 1 (Component)
Modified lambda Prm promoter (OR-3 obliterated)

OmpR
BBa_R0083 Version 1 (Component)
Promoter (OmpR, positive)

Prm +
BBa_I12006 Version 1 (Component)
Modified lamdba Prm promoter (repressed by 434 cI)

BBa_J44001
BBa_J44001 Version 1 (Component)
Reverse RBS (RBS_rev) -- corresponds to BBa_B0030

CD4 signal
BBa_I712009 Version 1 (Component)
CD4 signal peptide; localizes to plasma membrane

BBa_K1906010
BBa_K1906010 Version 1 (Component)
Ribothermometer A1

sfGFP_T65C
BBa_K1362170 Version 1 (Component)
Split sfGFP reconstitution positive control

BBa_K638001
BBa_K638001 Version 1 (Component)
Reflectin A1 from Loligo

BBa_M0100
BBa_M0100 Version 1 (Component)
tRNA_Arg5 to use as transcriptional reporter

BBa_K239006
BBa_K239006 Version 1 (Component)
Modified NarK promoter

BBa_K1906021
BBa_K1906021 Version 1 (Component)
ribothermometer

lac Or2-62
BBa_I12210 Version 1 (Component)
plac Or2-62 (positive)

BBa_I751500
BBa_I751500 Version 1 (Component)
pcI (for positive control of pcI-lux hybrid promoter)

BBa_K216005
BBa_K216005 Version 1 (Component)
PyeaR promoter, responsive to nitrate, nitrite and nitric oxide

BBa_K762002
BBa_K762002 Version 1 (Component)
TCC Positive Control

PelB
BBa_J32015 Version 1 (Component)
PelB leader sequence; directs protein to E. coli periplasmic membrane

BBa_K1906016
BBa_K1906016 Version 1 (Component)
Ribothermometer JB1_B5

BBa_K2066508
BBa_K2066508 Version 1 (Component)
Modified pLacO-1 Promoter (Lou et. al 2012)

BBa_I12036
BBa_I12036 Version 1 (Component)
Modified lamdba Prm promoter (cooperative repression by 434 cI)

tctA_503aa
BBa_K808002 Version 1 (Component)
tctA_503: large subunit A1 of the tripartite tricarboxylate transporter family

BBa_E3030
BBa_E3030 Version 1 (Component)
FRET acceptor YPet (optimized to work with CyPet (Cyan FP) as donor

BBa_K1331004
BBa_K1331004 Version 1 (Component)
Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6

BBa_K235028
BBa_K235028 Version 1 (Component)
[K235024][K235001] (NAND gate control test, positive control)

BBa_K1906015
BBa_K1906015 Version 1 (Component)
Ribothermometer JB1-B2

BBa_K1906013
BBa_K1906013 Version 1 (Component)
Ribothermometer JB2-C6

BBa_K1906017
BBa_K1906017 Version 1 (Component)
Ribothermometer JB2-B6

BBa_K1906014
BBa_K1906014 Version 1 (Component)
Ribothermometer JB1-A8

BBa_K1906018
BBa_K1906018 Version 1 (Component)
Ribothermometer JB1-G3

JB2-B1
BBa_K1906011 Version 1 (Component)
Ribothermometer JB2-B1

BBa_K1331001
BBa_K1331001 Version 1 (Component)
Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6

OmpA
BBa_K103006 Version 1 (Component)
OmpA outer membrane protein A fused to linker; displays proteins on cell surface

BBa_M36926
BBa_M36926 Version 1 (Component)
Beta-glucosidase A1

BBa_K235026
BBa_K235026 Version 1 (Component)
[K235022][K235001] (NAND gate control test, pLac positive control)

BBa_K235027
BBa_K235027 Version 1 (Component)
[K235024][K235000] (NAND gate control test, arabinose positive control)

BBa_K568007
BBa_K568007 Version 1 (Component)
GFP under constitutive promoter - positive control

BBa_Z0294
BBa_Z0294 Version 1 (Component)
A1,A2,A3,boxA

BBa_K607038
BBa_K607038 Version 1 (Component)
Prm modified promoter generating GFP

BBa_M31510
BBa_M31510 Version 1 (Component)
BBa_M13010 modified to remove g5 promoter

BBa_I715043
BBa_I715043 Version 1 (Component)
HPP-A1

BBa_K2082112
BBa_K2082112 Version 1 (Component)
Modified Pbad

BBa_I715049
BBa_I715049 Version 1 (Component)
HPP-A1 + Hin

BBa_K1461221
BBa_K1461221 Version 1 (Component)
ecf20 generator (positive feedback)

BBa_K1461222
BBa_K1461222 Version 1 (Component)
ecf11 generator (positive feedback)