IBMc334
IBMc334 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEY-e-NpuNcage-FKBP-T-cat-PBAD(SapI-)-RiboJ-BCD1-FRB-NpuCcage-CFNS-mCherry*(193-236)-H6-Ter

IBMc335
IBMc335 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEY-e-NpuNcage-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-NpuCcage-CFNS-mCherry*(193-236)-H6-Ter

BBa_K316027
BBa_K316027 Version 1 (Component)
B.subtilis transformation vector with LacI

BBa_K1758312
BBa_K1758312 Version 1 (Component)
Chromium responsive promoter with UTR+RBS and sfGFP

BBa_K1113001
BBa_K1113001 Version 1 (Component)
pSB4K5 with a promoter and RBS

BBa_K1419002
BBa_K1419002 Version 1 (Component)
RNA-OUT component for an Arabinose inducible lysis casette

BBa_K2088005
BBa_K2088005 Version 1 (Component)
pbaC with double Terminator

BBa_K1172306
BBa_K1172306 Version 1 (Component)
Riboflavin synthesis gene cluster from s. oneidensis under control of a strong Anderson promoter

BBa_K1172305
BBa_K1172305 Version 1 (Component)
Riboflavin synthesis gene cluster from s. oneidensis under control of a medium Anderson promoter

BBa_K1351025
BBa_K1351025 Version 1 (Component)
P_<i>comAB</i>, a CSP inducible promoter derived from <i>Streptococcus pneumoniae</i>

BBa_K1351024
BBa_K1351024 Version 1 (Component)
P_<i>comC</i>, a CSP inducible promoter derived from <i>Streptococcus pneumoniae</i>

BBa_K730001
BBa_K730001 Version 1 (Component)
The mxaF gene is approximately 1.8 kb in size and encodes a 66-kDa polypeptide.

BBa_K1687001
BBa_K1687001 Version 1 (Component)
An enhanced lacI protein generator, inhibits Plac perfectly

BBa_K557003
BBa_K557003 Version 1 (Component)
luxpR+cI: for modulating the ratio of Toggle switch

BBa_K118018
BBa_K118018 Version 1 (Component)
rbs+glgC16 (glgC with G336D substitution)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_R4030
BBa_R4030 Version 1 (Component)
PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)

BBa_K887004
BBa_K887004 Version 1 (Component)
Plac+alsS+ilvC+ilvD(each preceded by own zinc-finger and RBS)+Ptet+B0032+kivD+B0015

IodoY RS
BBa_K1416001 Version 1 (Component)
The tRNA synthetase/tRNA needed for incorporating 3-iodo-L-tyrosine (IodoY) at a UAG codon

BBa_K199011
BBa_K199011 Version 1 (Component)
RFP with CCCUC Addition

BBa_K199034
BBa_K199034 Version 1 (Component)
RFP with CGGUC Addition

rbs + sam5
BBa_K238007 Version 1 (Component)
Sam5 gene with RBS

BBa_M1105
BBa_M1105 Version 1 (Component)
Pyrene degradation enzyme RBS

BBa_M36925
BBa_M36925 Version 1 (Component)
Bicistronic RBS (medium) apFAB682

BBa_K783050
BBa_K783050 Version 1 (Component)
This is a MoClo converted version of BBa_B0033

BBa_K1897039
BBa_K1897039 Version 1 (Component)
Scar site generated by 3A Assembly

Nsense
BBa_M36356 Version 1 (Component)
A sensor that activates in the presence of nitrate in anaerobic conditions in E. coli.

BBa_K342003
BBa_K342003 Version 1 (Component)
OmpR234 protein, with higher effect on Curli promoter

BBa_K812032
BBa_K812032 Version 1 (Component)
mCFP with kozak sequence for eukaryotic expression

BBa_K541596
BBa_K541596 Version 1 (Component)
Reflectin gene with J04500 promoter

BBa_K1351039
BBa_K1351039 Version 1 (Component)
P_<i>xylA</i>, a xylose inducible promoter for <i>Bacillus subtilis</i>

BBa_K1819008
BBa_K1819008 Version 1 (Component)
Promoter test circuit to analyze the kill switch efficiency

BBa_K1321111
BBa_K1321111 Version 1 (Component)
Phytochelatin (PC) EC20 fused with CBDclos with LacI promoter

BBa_K1159106
BBa_K1159106 Version 1 (Component)
PhyB(2-908 AA)_36AALinker_C-TEV-Protease (First part for a light-switchable TEV-Protease) in RFC[25]

BBa_K1321138
BBa_K1321138 Version 1 (Component)
Metallothionein SmtA with T7 promoter

BBa_K909006
BBa_K909006 Version 1 (Component)
Full-length lacZ with RBS and Terminator

BBa_K843003
BBa_K843003 Version 1 (Component)
Lambda cI regulated by PLacI

PhyB-908-(
BBa_K365011 Version 1 (Component)
Phytochrome B (aa 1-908) linked to a trimer of ClpX (aa 61-425)

PB642(L-C)
BBa_K365010 Version 1 (Component)
Phytochrome B (aa 1-642) linked to a trimer of ClpX (aa 61-425)

BBa_K1051351
BBa_K1051351 Version 1 (Component)
K1051301 (clb2 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator)

BBa_K352011
BBa_K352011 Version 1 (Component)
pCooM Promoter coupled with RBS+RFP+Double terminator

BBa_R2109
BBa_R2109 Version 1 (Component)
Promoter with operator site for C2003

BBa_I759011
BBa_I759011 Version 1 (Component)
pTet-Q with L3

BBa_K1493802
BBa_K1493802 Version 1 (Component)
Promoter with Tet operator site

HO1
BBa_K646000 Version 1 (Component)
Hemeoxygenase with RBS

BBa_K1361006
BBa_K1361006 Version 1 (Component)
Curli Fiber generator where CsgBtrunc, a dissociative nucleator, under the control of Pbad promoter

BBa_K1051352
BBa_K1051352 Version 1 (Component)
K1051302 (clb5 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator)

BBa_K1051353
BBa_K1051353 Version 1 (Component)
K1051303 (clb6 promoter) + K1051000 (non stop codon RFP) + K1051008 (Stop codon + CAA terminator)

BBa_K1051363
BBa_K1051363 Version 1 (Component)
K1051303 (clb6 promoter) + K1051002 (non stop codon EYFP) + K1051008 (Stop codon + CAA terminator)