BBa_K1641007
BBa_K1641007 Version 1 (Component)
Fusion protein of Scre::EGFP::ssra-tag, with RBS at beginning

BBa_K1965017
BBa_K1965017 Version 1 (Component)
CaM(E104Q):Ctev

BBa_J31016
BBa_J31016 Version 1 (Component)
part produces the RNA construct crRNA-RBS-GFPLVA-tt that can only be translated in the presence of t

BBa_K1641009
BBa_K1641009 Version 1 (Component)
Fusion protein of Vika-EGFP-ssra, with RBS at beginning

BBa_K648101
BBa_K648101 Version 1 (Component)
RecA (mutated from RecA1 at amino acid 160 G-->A

BBa_K188006
BBa_K188006 Version 1 (Component)
Expression of ccdB for self-destruction of bacteria. Since promoter lux/cIIp22, this sequence can be

BBa_K594015
BBa_K594015 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces lasI and EYFP

(L-C)3
BBa_K365014 Version 1 (Component)
ClpX trimer with built-in linker at N-ter end

BBa_M45689
BBa_M45689 Version 1 (Component)
Constitutive promoter with a retinoic acid response element at the end

BBa_K987000
BBa_K987000 Version 1 (Component)
This part is a coding part that produces Vip3Ca3, a protein that can deals with different forms of p

BBa_K806003
BBa_K806003 Version 1 (Component)
SeqA regulation of chromosome replication by preventing re-initiation at newly replicated origins

BBa_K1088053
BBa_K1088053 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_J24822
BBa_J24822 Version 1 (Component)
Same as J24819 but with the error at the luc-terminator junction fixed

BBa_K855006
BBa_K855006 Version 1 (Component)
pvdQ gene with a silent mutation at 1494 bp to remove the internal PstI site

BBa_K855005
BBa_K855005 Version 1 (Component)
pvdQ gene with a silent mutation at 1491 bp to remove the internal PstI site

BBa_J119408
BBa_J119408 Version 1 (Component)
Pupp promoter mutant - Substitution of C and G to A at 28 and 30

BBa_K202004
BBa_K202004 Version 1 (Component)
Hybrid promoter having multiple operator sites. Promoter has tetO2 with mutation at position 3

BBa_K594011
BBa_K594011 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces 3-O-C12-HSL and ECFP reporter.

BBa_K1088059
BBa_K1088059 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_K1088052
BBa_K1088052 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_K594014
BBa_K594014 Version 1 (Component)
A device that can accepts the 3--OH-C14:1-HSL and then produces 3-O-C6-HSL and GFP reporter.

BBa_K1178000
BBa_K1178000 Version 1 (Component)
tRNA and synthetase for 3,4-dihydroxy-L-phenylalanine (L-DOPA) incorporation at UAG codon

IodoY RS
BBa_K1416001 Version 1 (Component)
The tRNA synthetase/tRNA needed for incorporating 3-iodo-L-tyrosine (IodoY) at a UAG codon

BBa_K1361005
BBa_K1361005 Version 1 (Component)
CsgE, CsgF, CsgG, the outer membrane secrete device for curli fiber, at relatively low constitutive

BBa_K1361007
BBa_K1361007 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative

BBa_K1412088
BBa_K1412088 Version 1 (Component)
A combination of theophylline aptamer and taRNA that can response theophylline to regulate circuit

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.