BBa_M12064
BBa_M12064 Version 1 (Component)
human MRC2

rbs+LIMS1
BBa_I742111 Version 1 (Component)
Limonene synthase gene with ribosome binding site

BBa_I742112
BBa_I742112 Version 1 (Component)
enoyl CoA hydratase (ech) gene

dCas 9
BBa_K1051800 Version 1 (Component)
protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.

BBa_J17001
BBa_J17001 Version 1 (Component)
G-alpha-olf

BBa_K1891010
BBa_K1891010 Version 1 (Component)
YCE+alpha-tubulin

BBa_K782060
BBa_K782060 Version 1 (Component)
Interferon alpha-2a

402
BBa_K398402 Version 1 (Component)
Prefoldin-alpha generator

BBa_K1891009
BBa_K1891009 Version 1 (Component)
YNE+alpha-tubulin

BBa_J17003
BBa_J17003 Version 1 (Component)
G-alpha-olf

BBa_K1891007
BBa_K1891007 Version 1 (Component)
alpha-tubulin+YNE

BBa_K1891008
BBa_K1891008 Version 1 (Component)
alpha-tubulin+YCE

BBa_K539742
BBa_K539742 Version 1 (Component)
TetR repressible promoter (BBa_R0040)+ kivd (alpha-ketoisovalerate decarboxylase)+Ter (BBa_B0015)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K2185006
BBa_K2185006 Version 1 (Component)
S.ferax alpha amylase coding sequence

rbs+COMT
BBa_I742109 Version 1 (Component)
COMT gene with ribosome binding site

Intein
BBa_K2075025 Version 1 (Component)
Intein N- and C-Term + Linker

BBa_M1269
BBa_M1269 Version 1 (Component)
Human Kappa Opioid Receptor

BBa_M1456
BBa_M1456 Version 1 (Component)
Mu Opioid receptor (human)

BBa_K1871001
BBa_K1871001 Version 1 (Component)
Alpha amylase with native secretion

BBa_K1590002
BBa_K1590002 Version 1 (Component)
Human Haptoglobin

BBa_K1104100
BBa_K1104100 Version 1 (Component)
mngB - alpha-mannosidase

BBa_K368002
BBa_K368002 Version 1 (Component)
Human Bak + SV40 Polyadenylation site

BBa_K1360001
BBa_K1360001 Version 1 (Component)
alpha-L-arabinofuranosidase ArfB

Fusion
BBa_I12201 Version 1 (Component)
Alpha-NTD - Gal11p fusion protein

BBa_K1441008
BBa_K1441008 Version 1 (Component)
PGAPz alpha A + RFP

BBa_K1590006
BBa_K1590006 Version 1 (Component)
Human Lanosterol Synthase

BBa_K1014001
BBa_K1014001 Version 1 (Component)
HrpR Gene

BBa_K110004
BBa_K110004 Version 1 (Component)
Alpha-Cell Promoter Ste3

BBa_K2185001
BBa_K2185001 Version 1 (Component)
Penicillium alpha amylase coding sequence

Cph8 gene
BBa_K566026 Version 1 (Component)
Cph8 gene for red-photoreceptor (constitutive expression)

BBa_K1792003
BBa_K1792003 Version 1 (Component)
alpha-Dextranase, secreted - Basic part

BBa_K850000
BBa_K850000 Version 1 (Component)
AGM1 gene from candida albicans

BBa_K094180
BBa_K094180 Version 1 (Component)
lacZ alpha fragment

BBa_K1792002
BBa_K1792002 Version 1 (Component)
alpha-Dextranase, secreted

CYP11A1
BBa_K1598008 Version 1 (Component)
J23101 promoter + RBS + Human FDXR + RBS + Human FDX1 + RBS + Human CYP11A1 + rrnb double terminator

BBa_K609002
BBa_K609002 Version 1 (Component)
human collagen type Ⅲ

BBa_C7053
BBa_C7053 Version 1 (Component)
T7 Gene 5.3

BBa_K364001
BBa_K364001 Version 1 (Component)
Human VDR DBD

BBa_M36037
BBa_M36037 Version 1 (Component)
Alpha Factor Mating Pheromone Producer

BBa_K258010
BBa_K258010 Version 1 (Component)
Human Epidermal growth factor (hEGF)

BBa_K1800001
BBa_K1800001 Version 1 (Component)
Alpha-Factor Secretion Signal

mFalpha
BBa_K1829006 Version 1 (Component)
Mating Factor Alpha (mFalpha)

rib-synth
BBa_K1172303 Version 1 (Component)
Riboflavin synthesis gene cluster from shewanella oneidensis

BBa_K2005002
BBa_K2005002 Version 1 (Component)
LacZ-alpha, UV-maximized

BBa_K1891005
BBa_K1891005 Version 1 (Component)
alpha-tubulin+N-Luciferase

BBa_K1441002
BBa_K1441002 Version 1 (Component)
PGAPz alpha A + Linker

BBa_K1891006
BBa_K1891006 Version 1 (Component)
C-Luciferase+alpha-tubulin