BBa_K1916014
BBa_K1916014 Version 1 (Component)
CBCR4 + intein-CBD tag

BBa_K1916000
BBa_K1916000 Version 1 (Component)
NpF2164g5 + intein-CBD tag

BBa_K1916003
BBa_K1916003 Version 1 (Component)
Cha6605_4344g2 + intein-CBD tag

BBa_K1916006
BBa_K1916006 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1435000
BBa_K1435000 Version 1 (Component)
4-HT inducible intein

BBa_K1916002
BBa_K1916002 Version 1 (Component)
Cyan7427_1390g3 + intein-CBD tag

BBa_K1916021
BBa_K1916021 Version 1 (Component)
CBCR9 + intein-CBD tag

BBa_K1916004
BBa_K1916004 Version 1 (Component)
Ga0100955_11928g3 + intein-CBD tag

BBa_K1916016
BBa_K1916016 Version 1 (Component)
CBCR6 + intein-CBD tag

BBa_K1916012
BBa_K1916012 Version 1 (Component)
CBCR3 + intein-CBD tag

BBa_K1319016
BBa_K1319016 Version 1 (Component)
TEV protease cleavage site

BBa_J70628
BBa_J70628 Version 1 (Component)
RFC12 Sce VMA Intein Internal Domain

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_I712668
BBa_I712668 Version 1 (Component)
CMV - HIV protease

BBa_K1362160
BBa_K1362160 Version 1 (Component)
RBS + Gp41-1 N-intein RFC[105] assembly construct (with His6)

RGK
BBa_K1362434 Version 1 (Component)
RGK (extein)

BBa_K1679022
BBa_K1679022 Version 1 (Component)
KIG, kcoil+intein+gfp

BBa_K1679027
BBa_K1679027 Version 1 (Component)
VMA, a thermosensitive intein

tphC_322aa
BBa_K808001 Version 1 (Component)
tphC: terephtalate periplasmatic binding proteine of the tripartite transporter family

3xSWAG(N)
BBa_K1362900 Version 1 (Component)
N-terminal tripleSWAGtag (N-intein)

BBa_K1679037
BBa_K1679037 Version 1 (Component)
VMA Dead, an inactivated intein

BBa_K1362901
BBa_K1362901 Version 1 (Component)
C-terminal tripleSWAGtag (C-intein)

AraC_14_3C
BBa_K627011 Version 1 (Component)
Fusion part of pBAD arabinose-inducible induction system and the HRV 14_3C protease

AraC_TEV-F
BBa_K627009 Version 1 (Component)
Arabinose inducible TEV protease

BBa_K1256006
BBa_K1256006 Version 1 (Component)
pSB1C3-Plac-SS-Protease

BBa_K1680011
BBa_K1680011 Version 1 (Component)
HCV protease with linkers

BBa_K1319008
BBa_K1319008 Version 1 (Component)
IPTG-induced and T7-driven expression of TEV protease

BBa_K1491027
BBa_K1491027 Version 1 (Component)
T7 RNA Polymerase with Dead ER Intein

SspDnaB(N*
BBa_K1362142 Version 1 (Component)
RBS + SspDnaB N-intein RFC[105] non-splicing control construct (with His6)

BBa_K380010
BBa_K380010 Version 1 (Component)
Immunoglobulin G protease (IdeS)

BBa_K1328003
BBa_K1328003 Version 1 (Component)
Insulin (modified protease recognition sites)

BBa_K316017
BBa_K316017 Version 1 (Component)
LacI operator controlled TEV protease

BBa_K533002
BBa_K533002 Version 1 (Component)
OmpA-HIV protease site-SH3

BBa_K185002
BBa_K185002 Version 1 (Component)
Lon Protease+double terminater

BBa_K1051704
BBa_K1051704 Version 1 (Component)
Mer2 Intron

BBa_K743013
BBa_K743013 Version 1 (Component)
Arabinose --> HIV protease secretion

BBa_K1680012
BBa_K1680012 Version 1 (Component)
Cleavage site for HCV protease

BBa_K2172007
BBa_K2172007 Version 1 (Component)
GST-Thrombin Protease-TEV-GFP

BBa_K2017000
BBa_K2017000 Version 1 (Component)
C-split Cas9 + DnaE C-intein

BBa_K2017001
BBa_K2017001 Version 1 (Component)
N-split Cas9 + DnaE N-intein

BBa_K185003
BBa_K185003 Version 1 (Component)
RelE toxin+Lon protease

BBa_K1362460
BBa_K1362460 Version 1 (Component)
RGK (extein)

BBa_K1638036
BBa_K1638036 Version 1 (Component)
Sce VMA Intein with promoter

BBa_K185050
BBa_K185050 Version 1 (Component)
Mutated Lon protease + double terminator

BBa_K2172008
BBa_K2172008 Version 1 (Component)
GST-Thrombin Protease-SmCPS1-TEV-GFP

BBa_K1159106
BBa_K1159106 Version 1 (Component)
PhyB(2-908 AA)_36AALinker_C-TEV-Protease (First part for a light-switchable TEV-Protease) in RFC[25]

BBa_K511701
BBa_K511701 Version 1 (Component)
B-Arrestin-2-TEV-Protease MammoBlock

Bar1
BBa_K1829000 Version 1 (Component)
Bar1: Protease that cleaves alpha factor

BBa_K627016
BBa_K627016 Version 1 (Component)
TEV protease from Tobacco Etch Virus

BBa_K1362399
BBa_K1362399 Version 1 (Component)
mutated (c-terminal Ser ->Ala) NpuDnaE C-Intein cloning piece