BBa_K094170
BBa_K094170 Version 1 (Component)
lacZ alpha

BBa_K1332001
BBa_K1332001 Version 1 (Component)
Histidine tag (8 AA) and RFP

BBa_K1460002
BBa_K1460002 Version 1 (Component)
RBS + GST (glutathione-S-transferase)-CRS5 (metallothionein) + Ter

IBMc318
IBMc318 Version 1 (Component)
pSB1A3-BbsI-G-ER-LBD-S-SapI

BBa_K654058
BBa_K654058 Version 1 (Component)
Thioesterase (TesA from E. coli) with 8-His Tag

BBa_J17001
BBa_J17001 Version 1 (Component)
G-alpha-olf

BBa_K1891010
BBa_K1891010 Version 1 (Component)
YCE+alpha-tubulin

BBa_K782060
BBa_K782060 Version 1 (Component)
Interferon alpha-2a

402
BBa_K398402 Version 1 (Component)
Prefoldin-alpha generator

BBa_K1891009
BBa_K1891009 Version 1 (Component)
YNE+alpha-tubulin

BBa_J17003
BBa_J17003 Version 1 (Component)
G-alpha-olf

BBa_K1891007
BBa_K1891007 Version 1 (Component)
alpha-tubulin+YNE

BBa_K1891008
BBa_K1891008 Version 1 (Component)
alpha-tubulin+YCE

BBa_K539742
BBa_K539742 Version 1 (Component)
TetR repressible promoter (BBa_R0040)+ kivd (alpha-ketoisovalerate decarboxylase)+Ter (BBa_B0015)

BBa_K861351
BBa_K861351 Version 1 (Component)
PfadR with S-FadA, intermediate part for BBa_K861048

BBa_J24716
BBa_J24716 Version 1 (Component)
36. pLacI=W RBS=AraC=S RBS=TetR=S RBS=mCherry(+LVA)=terminator

BBa_J64100
BBa_J64100 Version 1 (Component)
Medium copy ColE1 BioBrick vector

BBa_K1344008
BBa_K1344008 Version 1 (Component)
TolC-HlyB-HlyD Canal for Hemolysin-tag Secretion

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K2185006
BBa_K2185006 Version 1 (Component)
S.ferax alpha amylase coding sequence

λOtag
BBa_K365008 Version 1 (Component)
Lambda tag

BBa_K2018003
BBa_K2018003 Version 1 (Component)
Thuricin S

Spo0A
BBa_J01004 Version 1 (Component)
Spo0A Transcription Factor

IGPD
BBa_K1154002 Version 1 (Component)
Saccharomyces cerevisiae imidazoleglycerol-phosphate dehydratase (IGPD)

BBa_K2143001
BBa_K2143001 Version 1 (Component)
NahR_L154F Transcription Factor

BBa_K362004
BBa_K362004 Version 1 (Component)
sodA promoter is controlled by some transcription factors including SoxS.

BBa_K1453407
BBa_K1453407 Version 1 (Component)
Enzyme USB(AarI)-TAL USB-His Tag

BBa_K1453406
BBa_K1453406 Version 1 (Component)
Enzyme USB(BsmAI)-TAL USB-His Tag

BBa_K1728022
BBa_K1728022 Version 1 (Component)
LUCY tag

BBa_J331010
BBa_J331010 Version 1 (Component)
Linking Sequence for Metallothionein (CRS5) and Glutathione S-Transferase (GST)

HA tag
BBa_T2011 Version 1 (Component)
hemagglutinin (HA) tag special internal domain (YPYDVPDYA)

Strep-tag
BBa_T2014 Version 1 (Component)
Strep-tag II special internal domain (WSHPQFEK)

V5 tag
BBa_T2020 Version 1 (Component)
V5 epitope tag special internal domain (GKPIPNPLLGLDST)

HA tag
BBa_T2010 Version 1 (Component)
hemagglutinin (HA) tag head domain (YPYDVPDYA)

V5 tag
BBa_T2019 Version 1 (Component)
V5 epitope tag head domain (GKPIPNPLLGLDST)

Strep-tag
BBa_T2013 Version 1 (Component)
Strep-tag II head domain (WSHPQFEK)

BBa_I739001
BBa_I739001 Version 1 (Component)
Constitutive expression cassette for TetR +LVA (J23100.B0034.C0040.B0015)

BBa_J70029
BBa_J70029 Version 1 (Component)
RBS + mCherry gene, optimized for expression in Me. florum and E. coli

BBa_K2151002
BBa_K2151002 Version 1 (Component)
p32, strong constitutive <i>S. thermophilus</i> promoter

BBa_K2151001
BBa_K2151001 Version 1 (Component)
p25, moderate constitutive <i>S. thermophilus</i> promoter

BBa_K1871001
BBa_K1871001 Version 1 (Component)
Alpha amylase with native secretion

BBa_J120015
BBa_J120015 Version 1 (Component)
Coding sequence for PAX3, a regulatory transcription factor in H. sapiens

BBa_I741019
BBa_I741019 Version 1 (Component)
Right facing promoter (for xylA) controlled by xylR and CRP-cAMP

HA tag
BBa_T2009 Version 1 (Component)
hemagglutinin (HA) tag tail domain (YPYDVPDYA)

Strep-tag
BBa_T2012 Version 1 (Component)
Strep-tag tail domain (WRHPQFGG)

V5 tag
BBa_T2018 Version 1 (Component)
V5 epitope tag tail domain (GKPIPNPLLGLDST)

BBa_J73999
BBa_J73999 Version 1 (Component)
plasmid vector for Rhodobacter sphaeroides

BBa_J73998
BBa_J73998 Version 1 (Component)
plasmid vector for Rhodobacter sphaeroides

BBa_K1104100
BBa_K1104100 Version 1 (Component)
mngB - alpha-mannosidase

Scar25 TAA
BBa_K1319107 Version 1 (Component)
RFC 25 Translation Stop Scar Sequence