Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

lacI+pL
BBa_R0011 Version 1 (Component)
Promoter (lacI regulated, lambda pL hybrid)

cI lam
BBa_R0051 Version 1 (Component)
promoter (lambda cI regulated)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

luxI
BBa_C0061 Version 1 (Component)
autoinducer synthetase for AHL

BBa_J04450
BBa_J04450 Version 1 (Component)
RFP Coding Device

BBa_K648029
BBa_K648029 Version 1 (Component)
OR, operating region for Lambda switch

Prm +
BBa_I12007 Version 1 (Component)
Modified lambda Prm promoter (OR-3 obliterated)

cI+luxR
BBa_R0065 Version 1 (Component)
Promoter (lambda cI and luxR regulated -- hybrid)

GFP report
BBa_E0240 Version 1 (Component)
GFP generator

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

SEGA021
SEGA021 Version 1 (Component)
natural linear DNA.

mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

SEGA022
SEGA022 Version 1 (Component)
natural linear DNA.

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

GFP genera
BBa_E0840 Version 1 (Component)
GFP generator

OriTR
BBa_J01003 Version 1 (Component)
OriT-R (Origin of transfer for the R-plasmid nic region)

cI lam
BBa_R1051 Version 1 (Component)
Promoter, Standard (lambda cI regulated)<br>

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

BBa_P0451
BBa_P0451 Version 1 (Component)
PoPS -> cI (lambda) [S0153]

luxI
BBa_C0161 Version 1 (Component)
autoinducer synthetase for AHL (no LVA)

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

Prm +
BBa_I12006 Version 1 (Component)
Modified lamdba Prm promoter (repressed by 434 cI)

ccdB
BBa_K145151 Version 1 (Component)
ccdB coding region

BBa_E0430
BBa_E0430 Version 1 (Component)
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)

CII
BBa_K116602 Version 1 (Component)
CII coding region from &#955; phage

cI
BBa_I9200 Version 1 (Component)
cI lambda

cI857
BBa_J06503 Version 1 (Component)
Lambda cI857 (Lambda II) (RBS- LVA+)

BBa_P0151
BBa_P0151 Version 1 (Component)
PoPS -> cI (lambda) [S0165]

OL
BBa_K566000 Version 1 (Component)
OL region from Lambda

rhII
BBa_C0070 Version 1 (Component)
autoinducer synthetase for N-butyryl-HSL (BHL) and HHL

BBa_K1486000
BBa_K1486000 Version 1 (Component)
CpxR coding region

BBa_J23008
BBa_J23008 Version 1 (Component)
[key3c] riboregulator for lock3 variants

BBa_I13401
BBa_I13401 Version 1 (Component)
GFP reporter for RHS of library test constructs

lacI
BBa_Q04121 Version 1 (Component)
QPI (B0034.C0012.B0015.R0011)

BBa_K098997
BBa_K098997 Version 1 (Component)
cIts coding region

scar
BBa_G0000 Version 1 (Component)
SpeI/XbaI scar for RBS-CDS junctions

pLambda+RF
BBa_I763007 Version 1 (Component)
promoter lambda (cI regulated) with RFP reporter

BBa_J09855
BBa_J09855 Version 1 (Component)
Constitutive luxR device with pLuxR

BBa_K358006
BBa_K358006 Version 1 (Component)
lambda lysis cassette with terminator

BBa_I714031
BBa_I714031 Version 1 (Component)
OriT-R (Origin of Transfer for the R751-plasmid nic region)

lox
BBa_J61046 Version 1 (Component)
[Lox] site for recombination

BBa_K880005
BBa_K880005 Version 1 (Component)
Strong promoter, strong RBS combination for high expression levels of proteins

BBa_K1897030
BBa_K1897030 Version 1 (Component)
Lambda t0 terminator