BBa_K113005
BBa_K113005 Version 1 (Component)
pLac->tetR->pT7->tetR operator ->GFP

BBa_K105023
BBa_K105023 Version 1 (Component)
lac I - operator

BBa_K098984
BBa_K098984 Version 1 (Component)
lac inducible mtrB (high expression of QPI)

BBa_K098983
BBa_K098983 Version 1 (Component)
lac inducible mtrB (low expression of QPI)

Tm-betaLac
BBa_K157018 Version 1 (Component)
Tm-betaLactamaseBla1

Tm-betaLac
BBa_K157019 Version 1 (Component)
Tm-betaLactamase(Bla2)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K098986
BBa_K098986 Version 1 (Component)
lac QPI driven by high promoter

BBa_K098985
BBa_K098985 Version 1 (Component)
lac QPI driven by low promoter

BBa_K098981
BBa_K098981 Version 1 (Component)
tet QPI driven by high promoter

BBa_K098980
BBa_K098980 Version 1 (Component)
tet QPI driven by low promoter

BBa_K113007
BBa_K113007 Version 1 (Component)
pBad/araC->T7 polymerase

BBa_K113008
BBa_K113008 Version 1 (Component)
T7 polymerase

BBa_K113019
BBa_K113019 Version 1 (Component)
pBad/araC->T7 polymerase->GFP

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_S04138
BBa_S04138 Version 1 (Component)
cI QPI with GFP reporter system

BBa_K098988
BBa_K098988 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and high promoter

BBa_K098987
BBa_K098987 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and low promoter

BBa_K098982
BBa_K098982 Version 1 (Component)
lac inducible system with low promoter and GFP reporter

BBa_K113021
BBa_K113021 Version 1 (Component)
Total construct: T7 polymerase + GFP/RFP + repressors + overlapping T7 promoter

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SP-AntiNIP
BBa_K157024 Version 1 (Component)
SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla1)

SP-AntiNIP
BBa_K157025 Version 1 (Component)
SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla2)

SP-Lipocal
BBa_K157032 Version 1 (Component)
SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla1)

SP-Lipocal
BBa_K157033 Version 1 (Component)
SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla2)

rTT+pR
BBa_K093003 Version 1 (Component)
Convergent Promoter System: Forward Module: rTT+lambda pR

MioC
BBa_K101017 Version 1 (Component)
MioC Promoter (DNAa-Repressed Promoter)

B-H-R plas
BBa_K125020 Version 1 (Component)
lac promoter/rbs/rep/oriV/lac promoter/rbs/aadA/oriT

BBa_K156001
BBa_K156001 Version 1 (Component)
BioBrick Placeholder 1 (Prefix - ApoI (NotI) AvrII - Suffix)

BBa_K123002
BBa_K123002 Version 1 (Component)
LacIQ ERE TetR

BBa_K082004
BBa_K082004 Version 1 (Component)
LacI- wild type

BBa_K082005
BBa_K082005 Version 1 (Component)
LacI-Mutant

BBa_K082012
BBa_K082012 Version 1 (Component)
B0034-LacI

BBa_K082034
BBa_K082034 Version 1 (Component)
lacI regulated GFP generator

BBa_K156002
BBa_K156002 Version 1 (Component)
BioBrick placeholder 2 (Prefix - ApoI (NotI) NheI - Suffix)

BBa_K156003
BBa_K156003 Version 1 (Component)
BioBrick placeholder 3 (Prefix - ApoI (NotI) NsiI - Suffix)

BBa_K156004
BBa_K156004 Version 1 (Component)
BioBrick placeholder 4 (Prefix - ApoI (NotI) SbfI - Suffix)

BBa_K156005
BBa_K156005 Version 1 (Component)
BioBrick placeholder 5 (Prefix - MfeI (NotI) AvrII - Suffix)

BBa_K156006
BBa_K156006 Version 1 (Component)
BioBrick placeholder 6 (Prefix - MfeI (NotI) NheI - Suffix)

BBa_K086000
BBa_K086000 Version 1 (Component)
unmodified Lutz-Bujard LacO promoter - YFP

BBa_K086017
BBa_K086017 Version 1 (Component)
unmodified Lutz-Bujard LacO promoter

BBa_K156007
BBa_K156007 Version 1 (Component)
BioBrick placeholder 7 (Prefix - MfeI (NotI) Nsil - Suffix)

BBa_K156008
BBa_K156008 Version 1 (Component)
BioBrick placeholder 8 (Prefix - MfeI (NotI) SbfI - Suffix)

BBa_K086018
BBa_K086018 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24

BBa_K086019
BBa_K086019 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24

BBa_K086020
BBa_K086020 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24

BBa_K082028
BBa_K082028 Version 1 (Component)
R0040-B0034-LacI