tetRBBa_C0040 Version 1 (Component)tetracycline repressor from transposon Tn10 (+LVA)
lacIBBa_C0012 Version 1 (Component)lacI repressor from E. coli (+LVA)
lacI+pLBBa_R0011 Version 1 (Component)Promoter (lacI regulated, lambda pL hybrid)
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
mRFP1BBa_E1010 Version 1 (Component)**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
BBa_J23100BBa_J23100 Version 1 (Component)constitutive promoter family member
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
scarBBa_J176041 Version 1 (Component)Silver scar
lux pRBBa_R0062 Version 1 (Component)Promoter (luxR & HSL regulated -- lux pR)
cI lamBBa_C0051 Version 1 (Component)cI repressor from E. coli phage lambda (+LVA)
BBa_J23119BBa_J23119 Version 1 (Component)constitutive promoter family member
BBa_J04450BBa_J04450 Version 1 (Component)RFP Coding Device
pBad/araCBBa_I0500 Version 1 (Component)Inducible pBad/araC promoter
eyfpBBa_E0030 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
cI lamBBa_R0051 Version 1 (Component)promoter (lambda cI regulated)
luxrBBa_C0062 Version 1 (Component)luxR repressor/activator, (no LVA?)
BBa_B0031BBa_B0031 Version 1 (Component)RBS.2 (weak) -- derivative of BBa_0030
BBa_J23101BBa_J23101 Version 1 (Component)constitutive promoter family member
P(Lac) IQBBa_I14032 Version 1 (Component)promoter P(Lac) IQ
pSB1C3pSB1C3 Version 1 (Component)High copy BioBrick assembly plasmid
luxIBBa_C0061 Version 1 (Component)autoinducer synthetase for AHL
BBa_J23106BBa_J23106 Version 1 (Component)constitutive promoter family member
Amp CasBBa_I13800 Version 1 (Component)Ampicillin Resistance Cassette
BBa_J61101BBa_J61101 Version 1 (Component)Ribosome Binding Site Family Member
BBa_I13453BBa_I13453 Version 1 (Component)Pbad promoter
BBa_B0011BBa_B0011 Version 1 (Component)LuxICDABEG (+/-)
pT7BBa_I719005 Version 1 (Component)T7 Promoter
mChBBa_J176005 Version 1 (Component)mCh
BBa_B1001BBa_B1001 Version 1 (Component)Terminator (artifical, small, %T~=90)
GFP reportBBa_E0240 Version 1 (Component)GFP generator
YFPBBa_E0032 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
BBa_J04500BBa_J04500 Version 1 (Component)IPTG inducible promoter with RBS
BBa_B0014BBa_B0014 Version 1 (Component)double terminator (B0012-B0011)
ecfpBBa_E0020 Version 1 (Component)engineered cyan fluorescent protein derived from A. victoria GFP
SEGA021SEGA021 Version 1 (Component)natural linear DNA.
SEGA022SEGA022 Version 1 (Component)natural linear DNA.
BBa_B0033BBa_B0033 Version 1 (Component)RBS.4 (weaker) -- derivative of BBa_0030
Scar 25BBa_B0105 Version 1 (Component)RFC 25 Scar Sequence
BBa_J23110BBa_J23110 Version 1 (Component)constitutive promoter family member
BBa_J61120BBa_J61120 Version 1 (Component)Ribosome Binding Site Family Member
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
BBa_I13507BBa_I13507 Version 1 (Component)Screening plasmid intermediate
BBa_B1006BBa_B1006 Version 1 (Component)Terminator (artificial, large, %T~>90)
BBa_J56012BBa_J56012 Version 1 (Component)Invertible sequence of dna includes Ptrc promoter
GFP generaBBa_E0840 Version 1 (Component)GFP generator
CcpABO_11480 Version 1 (Component) cI+luxRBBa_R0065 Version 1 (Component)Promoter (lambda cI and luxR regulated -- hybrid)