BBa_K1051801
BBa_K1051801 Version 1 (Component)
Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib

LuxOU
BBa_K131016 Version 1 (Component)
LuxOU from Vibrio harveyi

ZEC
BBa_K1018001 Version 1 (Component)
she ble gene conferring Zeocin resistance

BBa_J85950
BBa_J85950 Version 1 (Component)
LacI inverter regulated by TRE

BBa_J147001
BBa_J147001 Version 1 (Component)
RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter

Nsense
BBa_M36356 Version 1 (Component)
A sensor that activates in the presence of nitrate in anaerobic conditions in E. coli.

BBa_K322237
BBa_K322237 Version 1 (Component)
Firefly luciferase from Photinus pyralis

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K362007
BBa_K362007 Version 1 (Component)
This composite part has the promoter ,ahpC-promoter controlled by OxyR protein, and GFP - T-T

LuxPQ
BBa_K131015 Version 1 (Component)
LuxPQ from Vibrio harveyi

BBa_J147000
BBa_J147000 Version 1 (Component)
RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter

BBa_J147002
BBa_J147002 Version 1 (Component)
RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter

BBa_K1189020
BBa_K1189020 Version 1 (Component)
Light chain human ferritin with his-tag and E-coil under the lacI inducible promoter

BBa_K582000
BBa_K582000 Version 1 (Component)
500bp of Spacer/Repeat Region of the CRISPR genome present in Strep. thermophilus LMD9 strain

BBa_K855003
BBa_K855003 Version 1 (Component)
coding sequence for pvdQ with histag added to the C-terminal followed by stop codon

BBa_K1189019
BBa_K1189019 Version 1 (Component)
Heavy chain human ferritin with his-tag and E-coil under the lacI inducible promoter

BBa_K1361005
BBa_K1361005 Version 1 (Component)
CsgE, CsgF, CsgG, the outer membrane secrete device for curli fiber, at relatively low constitutive

BBa_K1983012
BBa_K1983012 Version 1 (Component)
tRNA-Phe (pheU) under DH10B promoter and terminator

BBa_K1983011
BBa_K1983011 Version 1 (Component)
tRNA-Phe (pheU) under constitutive promoter and terminator

BBa_K079026
BBa_K079026 Version 1 (Component)
GFP reporter protein and LacI gene sequence under the control of J23118 constitutive promoter

BBa_J32028
BBa_J32028 Version 1 (Component)
HE Code

BBa_K1371009
BBa_K1371009 Version 1 (Component)
L9-TE

BBa_K1361006
BBa_K1361006 Version 1 (Component)
Curli Fiber generator where CsgBtrunc, a dissociative nucleator, under the control of Pbad promoter

BBa_K1607011
BBa_K1607011 Version 1 (Component)
The SCFV of anti-p185her2/neu antibody chA21 linked with EGFP by a (Gly4Ser)3 linker

BBa_K1132037
BBa_K1132037 Version 1 (Component)
AND-inverted RFP gate (BBa_K1132034) with T7 polymerase under the control of a strong promoter

BBa_K1132038
BBa_K1132038 Version 1 (Component)
XOR-inverted RFP gate (BBa_K1132035) with T7 polymerase under the control of a strong promoter

BBa_K1363003
BBa_K1363003 Version 1 (Component)
OriT-K592016 the blue sensor which can be transfered into other bacterias by S17-1

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

BBa_K831014
BBa_K831014 Version 1 (Component)
Inducible istR (inhibitor of SOS-induced toxicity by RNA) under the control of lac promoter

BBa_K1361002
BBa_K1361002 Version 1 (Component)
Curli Fiber generator where CsgBtrunc, a dissociative nucleator, under the control of Pbad promoter

BBa_K1861020
BBa_K1861020 Version 1 (Component)
Fusion protein of the TALE constant domain and a SpyTag in an expression cassette

BBa_K329003
BBa_K329003 Version 1 (Component)
Strong RBS (B0034) - Tetracycline resistance protein TetA(C) (J31007)

BBa_K1613017
BBa_K1613017 Version 1 (Component)
K1613017 is a new part which can detect lead (Pb) ions in the liquid.

BBa_K1647019
BBa_K1647019 Version 1 (Component)
Cap-AAP2, a improved antimicrobial peptide which can kill the S.mutans selectively and have changeab

BBa_K1618021
BBa_K1618021 Version 1 (Component)
Plac-THL-CRO-BHBD-BCD-EFTb-EFTa

BBa_K112217
BBa_K112217 Version 1 (Component)
{a~ihfA!} The ihf alpha gene ready to have rbs attached with stop codon, BBb format

BBa_K112211
BBa_K112211 Version 1 (Component)
{a~int!} The integrase gene with rbs ready to be attached with stop codon, BBb format

BBa_K1655001
BBa_K1655001 Version 1 (Component)
This GFP can be fused into any protein's aminoterminal end with the BioBrick enzyme assembly method.

BBa_K880001
BBa_K880001 Version 1 (Component)
Asymmetrically digestible reporter to assay the activity of DNA recombinases FimE K137007 and HbiF K

AckA
BBa_K737002 Version 1 (Component)
This coding is the acetate kinase.

BBa_K2176001
BBa_K2176001 Version 1 (Component)
Cassette for the constitutive production of GAL4-KaiCp and LexA-SasA, linked by a self-cleaving P2A

BBa_K1216014
BBa_K1216014 Version 1 (Component)
BBa_K1216014 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.

BBa_K1216012
BBa_K1216012 Version 1 (Component)
BBa_K1216012 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.

BBa_K1216013
BBa_K1216013 Version 1 (Component)
BBa_K1216013 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.

BBa_K1216011
BBa_K1216011 Version 1 (Component)
BBa_K1216007 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.

BBa_K1216010
BBa_K1216010 Version 1 (Component)
BBa_K1216010 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL. (