BBa_K737014BBa_K737014 Version 1 (Component)gvpC-GFP fusion protein UH
CBDclosBBa_K863111 Version 1 (Component)Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)
BBa_K873000BBa_K873000 Version 1 (Component)heat shock 10kDa protein(hsp 10) groES coding resquence
BBa_K739004BBa_K739004 Version 1 (Component)Ax21 protein - signaling part
pLac ColE2BBa_K914001 Version 1 (Component)pLac Colicin E2 Immunity protein
BBa_K914002BBa_K914002 Version 1 (Component)repressilator RBS Colicin E2 Immunity protein
BBa_K883160BBa_K883160 Version 1 (Component)CCMV_Negative coat protein
BBa_K883151BBa_K883151 Version 1 (Component)CCMV coat protein gene 25AA N-terminal replaced with HIStag under IPTG inducable promotor
gpSBBa_K836006 Version 1 (Component)Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K157010BBa_K157010 Version 1 (Component)glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac
linkerBBa_K157013 Version 1 (Component)15 aa flexible glycine-serine protein domain linker; Freiburg standard
BBa_K156009BBa_K156009 Version 1 (Component)OFP (orange fluorescent protein)
SBFP2BBa_K156010 Version 1 (Component)SBFP2 (strongly enhanced blue fluorescent protein)
BBa_I0466BBa_I0466 Version 1 (Component)RhlR Protein Generator
BBa_K116001BBa_K116001 Version 1 (Component)nhaA promoter, that can be regulated by pH and nhaR protein.
BBa_K079051BBa_K079051 Version 1 (Component)LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator
BBa_K079052BBa_K079052 Version 1 (Component)LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato
BBa_K127013BBa_K127013 Version 1 (Component)gas vesicle protein A from cyanobacteria Anabaena
BBa_J36835BBa_J36835 Version 1 (Component)Lipoprotein signal peptide
BBa_J36836BBa_J36836 Version 1 (Component)Outer membrane protein A, aa 46-66
BBa_K116102BBa_K116102 Version 1 (Component)IPTG inducible membrane protein generator
BBa_K129003BBa_K129003 Version 1 (Component)LamB-maltose transporter membrane protein
BBa_J70030BBa_J70030 Version 1 (Component)A BioScaffold Part (Uses PpiI), Protein Tail Remover see Part Design Page
BBa_J70032BBa_J70032 Version 1 (Component)A Composite BioScaffold Part (PpiI and PsrI) for Protein Fusions (see Design Page)
BBa_J70033BBa_J70033 Version 1 (Component)A BioMortar Part for Creating GSGS Protein Fusions (see Design page)
BBa_J69516BBa_J69516 Version 1 (Component)lacI-regulated tetR invertor Protein Generator
BBa_J70060BBa_J70060 Version 1 (Component)BBa_J70010 (PpiI) protein fusion test part
BBa_J70070BBa_J70070 Version 1 (Component)BBa_J70012 (PsrI) protein fusion test part
BBa_J70080BBa_J70080 Version 1 (Component)BBa_J70030 (PpiI) protein fusion test part
BBa_J70090BBa_J70090 Version 1 (Component)BBa_J70032 (PpiI/PsrI) protein fusion test part
BBa_J70061BBa_J70061 Version 1 (Component)BBa_J70009 (PpiI) protein fusion test part
tetA(C)fBBa_J31007 Version 1 (Component)tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
BBa_J70121BBa_J70121 Version 1 (Component)R0040 regulated expression of a lacZa.GFP fusion protein
BBa_M11006BBa_M11006 Version 1 (Component)ArsC arsenate reductase protein
BBa_M11011BBa_M11011 Version 1 (Component)Chloramphenicol Resistance Protein
BBa_M11080BBa_M11080 Version 1 (Component)silE (coding sequence for Silver binding protein)
BBa_M11081BBa_M11081 Version 1 (Component)cutA (coding sequence for copper binding protein)
BBa_M11076BBa_M11076 Version 1 (Component)NrtA: nitrate/nitrite binding protein coding region
BBa_M11036BBa_M11036 Version 1 (Component)D-galactose-binding periplasmic protein (D-galactose/ D-glucose-binding protein)
BBa_M11012BBa_M11012 Version 1 (Component)CheA Chemotaxis reception protein
BBa_M11078BBa_M11078 Version 1 (Component)OmpC + Copper-Binding Protein
BBa_M11083BBa_M11083 Version 1 (Component)MerP, coding sequence for mercuric ion binding protein
ompC w/CadBBa_M11084 Version 1 (Component)E coli outer membrane protein C (ompC) with Cadmium Binding protien
BBa_M11052BBa_M11052 Version 1 (Component)Bacteriorhodopsin Related Protein
BBa_M11085BBa_M11085 Version 1 (Component)E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o