BBa_K1465304
BBa_K1465304 Version 1 (Component)
<i>adhA</i> gene from <i>Lactococcus lactis</i> under <i>T7</i> control

BBa_K1465305
BBa_K1465305 Version 1 (Component)
<i>adhA</i> gene from <i>Lactococcus lactis</i> under <i>Ptac</i> control

BBa_K2116028
BBa_K2116028 Version 1 (Component)
bxb1 integrase under the control of nitric oxide and AHL responsive AND gate

BBa_K1051801
BBa_K1051801 Version 1 (Component)
Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1758322
BBa_K1758322 Version 1 (Component)
Copper activator under control constitutive promoter and strong RBS and Copper responsive promoter w

BBa_K1172306
BBa_K1172306 Version 1 (Component)
Riboflavin synthesis gene cluster from s. oneidensis under control of a strong Anderson promoter

BBa_K1172305
BBa_K1172305 Version 1 (Component)
Riboflavin synthesis gene cluster from s. oneidensis under control of a medium Anderson promoter

BBa_K1758202
BBa_K1758202 Version 1 (Component)
His-tagged lac repressor fused to superfolder GFP under the control of the T7 promoter

BBa_K2151008
BBa_K2151008 Version 1 (Component)
p32-I13500: GFP under control of strong constitutive promoter and strong ribosome binding site

BBa_K2151007
BBa_K2151007 Version 1 (Component)
p25-I13500: GFP under control of medium constitutive promoter and strong ribosome binding site

BBa_K2151006
BBa_K2151006 Version 1 (Component)
pHLBA-I13500: GFP under control of strong constitutive promoter and strong ribosome binding site

BBa_K1758353
BBa_K1758353 Version 1 (Component)
Nickel repressor under control of constitutive promoter and strong RBS and Nickel responsive promote

BBa_K658010
BBa_K658010 Version 1 (Component)
a bacteria population-control device with lux pR-5 driven by lacl+pL

BBa_K658009
BBa_K658009 Version 1 (Component)
a bacteria population-control device with lux pR-3 driven by lacl+pL

BBa_K079026
BBa_K079026 Version 1 (Component)
GFP reporter protein and LacI gene sequence under the control of J23118 constitutive promoter

BBa_K079024
BBa_K079024 Version 1 (Component)
LacI repressor under the control of the J23118 costitutive promoter and Lac 1 operator

BBa_K1361006
BBa_K1361006 Version 1 (Component)
Curli Fiber generator where CsgBtrunc, a dissociative nucleator, under the control of Pbad promoter

BBa_K1172304
BBa_K1172304 Version 1 (Component)
Riboflavin synthesis gene cluster from s. oneidensis under control of T7 promoter and strong RBS

BBa_K1132037
BBa_K1132037 Version 1 (Component)
AND-inverted RFP gate (BBa_K1132034) with T7 polymerase under the control of a strong promoter

BBa_K1132038
BBa_K1132038 Version 1 (Component)
XOR-inverted RFP gate (BBa_K1132035) with T7 polymerase under the control of a strong promoter

BBa_K2082231
BBa_K2082231 Version 1 (Component)
RFP under the control of an optimized lacZ promoter combined with the protein SH2:cI434

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

BBa_K831014
BBa_K831014 Version 1 (Component)
Inducible istR (inhibitor of SOS-induced toxicity by RNA) under the control of lac promoter

BBa_K1361002
BBa_K1361002 Version 1 (Component)
Curli Fiber generator where CsgBtrunc, a dissociative nucleator, under the control of Pbad promoter

BBa_K079021
BBa_K079021 Version 1 (Component)
LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La

BBa_K1039022
BBa_K1039022 Version 1 (Component)
PhiC31 integrase and PhiC31 RDF under lock and key control with lacI Expression Cassette

BBa_K1039021
BBa_K1039021 Version 1 (Component)
Bxb1 integrase and Recombination Directionality Factor (RDF) Under ???Lock and Key??? Control with L

BBa_K658011
BBa_K658011 Version 1 (Component)
a bacteria population-control device with lux pR-3/5 driven by lacl+pL

BBa_I731014
BBa_I731014 Version 1 (Component)
The luxR based receiver, F2620 (formerly I13270), controls the production of mCherry

BBa_K1361004
BBa_K1361004 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag

BBa_K2082237
BBa_K2082237 Version 1 (Component)
RFP and Tetracycline resistence(tetA) under the control of a modified lacZ promoter; Fusion protein

BBa_K079049
BBa_K079049 Version 1 (Component)
GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator

BBa_K079020
BBa_K079020 Version 1 (Component)
GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein

BBa_K1616003
BBa_K1616003 Version 1 (Component)
VVD link to YC155 (YFP Cter split)

BBa_K1616004
BBa_K1616004 Version 1 (Component)
VVD linked to YN155 (YFP Nter split)

BBa_K1189029
BBa_K1189029 Version 1 (Component)
TALE-A with a his tag linked to a K coil under the control of a LacI promoter

BBa_K1361003
BBa_K1361003 Version 1 (Component)
Curli Fiber generator under the control of T7 promoter with a relatively strong expression of CsgA,C

BBa_K1361001
BBa_K1361001 Version 1 (Component)
Curli Fiber generator under the control of T7 promoter with a relatively weak expression of CsgA,C

BBa_K812233
BBa_K812233 Version 1 (Component)
sfGFP in pCS2+ with elastase promoter

BBa_I13035
BBa_I13035 Version 1 (Component)
3OC₆HSL Receiver Device with Inducible Control of LuxR and a YFP Output device

BBa_K1361007
BBa_K1361007 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative

BBa_K2082252
BBa_K2082252 Version 1 (Component)
RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI

BBa_K812333
BBa_K812333 Version 1 (Component)
pCS2+ with HSP70 promoter and sfGFP reporter

BBa_K1321362
BBa_K1321362 Version 1 (Component)
sfGFP fused to CBDcex driven by LacI

BBa_K1541025
BBa_K1541025 Version 1 (Component)
sfGFP under promoter P(Lux) with riboregulator RR12y

BBa_K812133
BBa_K812133 Version 1 (Component)
sfGFP with kozak sequence for expression in Xenopus

BBa_K563010
BBa_K563010 Version 1 (Component)
Tor2 gene from the genome of the S. cerevisiae, central protein in TOR(target of rapamycin) pathway

BBa_K077041
BBa_K077041 Version 1 (Component)
AiiA and cII under control of plac promotor