BBa_M1719
BBa_M1719 Version 1 (Component)
Hepcidin and His-tag

BBa_J70614
BBa_J70614 Version 1 (Component)
RFC12 His tagged GFP

BBa_K864050
BBa_K864050 Version 1 (Component)
pSC101 low copy origin of replication

BBa_M12100
BBa_M12100 Version 1 (Component)
PLambda : B0034 : RFP in BioScaffold Form, a (0,5;7,2] BioScaffold part

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K106693
BBa_K106693 Version 1 (Component)
AarI A!D acceptor vector (pRS315, Cyc1P, Adh1t)

BBa_K1976055
BBa_K1976055 Version 1 (Component)
T7 generator of the trypsin fragment of the Colicin E2 DNase

pSB2K4
pSB2K4 Version 1 (Component)
pSB2K3-derived BioBrick plasmid free of many restriction sites

BBa_K957004
BBa_K957004 Version 1 (Component)
nah Operon for degradation of naphthalene to salicylate

BBa_K215261
BBa_K215261 Version 1 (Component)
Lpp + 5tmr OmpA + GS Linker + TEV site + WT Streptavidin + 6His

BBa_K1509001
BBa_K1509001 Version 1 (Component)
A bi-directional promoter affected by SmtB protein

BBa_K1675007
BBa_K1675007 Version 1 (Component)
a device to express the glutaminase1 constitutively

BBa_K1937008
BBa_K1937008 Version 1 (Component)
AF-A antifungal operon in pSBBs0K-mini

BBa_M31519
BBa_M31519 Version 1 (Component)
Modified promoter sequence of g3.

BBa_K2010006
BBa_K2010006 Version 1 (Component)
PETase Composite (T7, His-Tag)

BBa_K1481001
BBa_K1481001 Version 1 (Component)
his-mRFP under rhamnose promoter

BBa_K1991010
BBa_K1991010 Version 1 (Component)
Pcons-RBS-LO-GFP-His

BBa_K1022111
BBa_K1022111 Version 1 (Component)
PT7: RBS: Signiferin: His 6

BBa_K1312004
BBa_K1312004 Version 1 (Component)
pSB1C3 plus 6x His tag

BBa_K549022
BBa_K549022 Version 1 (Component)
pabA with fused His-tag

BBa_K1022130
BBa_K1022130 Version 1 (Component)
RBS: His-SUMO-Staphycine-TetR:TT

BBa_K2022003
BBa_K2022003 Version 1 (Component)
TolR, Periplasmic Domain, His-tag

BBa_K945004
BBa_K945004 Version 1 (Component)
Pichia pastoris optimized His-GFP

BBa_K652001
BBa_K652001 Version 1 (Component)
T7-RBS-RiAFP-His-Term

BBa_K1362203
BBa_K1362203 Version 1 (Component)
Sortase A circularization construct (with Smt3 and His6; without RBS)

BBa_K1351002
BBa_K1351002 Version 1 (Component)
Chondroitin 4 sulfotransferase-derived peptide binding to NADH Oxidase of S. pneumoniae

BBa_K518003
BBa_K518003 Version 1 (Component)
LexA - a repressor for LexA-regulated SOS promoters -

BBa_K648002
BBa_K648002 Version 1 (Component)
cI repressor under control of constitutive

BBa_K1963006
BBa_K1963006 Version 1 (Component)
A translational fusion between E. coli OsmY and Serratia Hfq

BBa_K549038
BBa_K549038 Version 1 (Component)
Promotor prcnA of rcnA with RcnR-binding site and 50nt rcnA

BBa_K1676116
BBa_K1676116 Version 1 (Component)
Mutant 12 of Lactate Dehydrogenase

BBa_K1957004
BBa_K1957004 Version 1 (Component)
HydA subunit of FeFe Hydrogenase

BBa_J72157
BBa_J72157 Version 1 (Component)
upp 3' UTR of MG1655

W+Immuno
BBa_K320012 Version 1 (Component)
Weak constitutive expression of Immunotoxin

BBa_K739003
BBa_K739003 Version 1 (Component)
promoter of raxRH + RBS + mRFP

BBa_J100307
BBa_J100307 Version 1 (Component)
Variant of repClone Red (J100205)

BBa_K1957005
BBa_K1957005 Version 1 (Component)
HyaA subunit of NiFe Hydrogenase

BBa_K1957001
BBa_K1957001 Version 1 (Component)
HydC subunit of FeFe Hydrogenase

BBa_K353008
BBa_K353008 Version 1 (Component)
sfGFP under control of pEMBR

BBa_J212000
BBa_J212000 Version 1 (Component)
strong promoter of Trichoderma reesei

BBa_J72156
BBa_J72156 Version 1 (Component)
upp 5' UTR of MG1655

W+p25
BBa_K320013 Version 1 (Component)
Weak constitutive expression of P25

BBa_J100018
BBa_J100018 Version 1 (Component)
First Half of AspC gene

BBa_J100025
BBa_J100025 Version 1 (Component)
First half of CAT gene

BBa_K189046
BBa_K189046 Version 1 (Component)
C-repressor of Bacteriophage Mu

TE3
BBa_K257022 Version 1 (Component)
TE3_fusion, domain of the colicin

BBa_K1957006
BBa_K1957006 Version 1 (Component)
HyaC subunit of NiFe Hydrogenase

BBa_G10001
BBa_G10001 Version 1 (Component)
Lux operon downstream of Plac

BBa_J100019
BBa_J100019 Version 1 (Component)
First half of ilvE gene

BBa_K1959000
BBa_K1959000 Version 1 (Component)
Modification of Phytoene synthase (PSY)