BBa_S01843
BBa_S01843 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01378
BBa_S01378 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01826
BBa_S01826 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01382
BBa_S01382 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01821
BBa_S01821 Version 1 (Component)
Intermediate part from assembly 240

BBa_K936022
BBa_K936022 Version 1 (Component)
Cutinase and polyhistidine tag

BBa_S02001
BBa_S02001 Version 1 (Component)
Intermediate part from assembly 240

LacY
BBa_K079014 Version 1 (Component)
LacY transporter protein from E. coli

BBa_K619894
BBa_K619894 Version 1 (Component)
mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K1447004
BBa_K1447004 Version 1 (Component)
Epitope 1-5 from Ara h 1

BBa_K1447005
BBa_K1447005 Version 1 (Component)
Epitope 1-5 from Ara h 1

BBa_K619895
BBa_K619895 Version 1 (Component)
mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K833002
BBa_K833002 Version 1 (Component)
mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K833006
BBa_K833006 Version 1 (Component)
Mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K833008
BBa_K833008 Version 1 (Component)
Mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K809321
BBa_K809321 Version 1 (Component)
GAL promoter + YFP_SPtim21 + ADH1 terminator

BBa_K809308
BBa_K809308 Version 1 (Component)
GAL promoter + RFP_SPzim17 + ADH1 terminator

BBa_K1323020
BBa_K1323020 Version 1 (Component)
oriV from the Staphylococcus aureus pSK41 plasmid clone 3

BBa_K1766008
BBa_K1766008 Version 1 (Component)
EnvZ_V5 osmoregulatory histidine kinase from <i>E.coli</i>.

BBa_K1766014
BBa_K1766014 Version 1 (Component)
EnvZ osmoregulatory histidine kinase from <i>E.coli.</i>

BBa_K1845001
BBa_K1845001 Version 1 (Component)
Miraculin (Yeast codon optimised + His-tag)

BBa_I724005
BBa_I724005 Version 1 (Component)
Elowitz repressilator with added degradation tag

BBa_K1497197
BBa_K1497197 Version 1 (Component)
B0034-CHI - Chalcone Isomerase from Petunia with strong RBS

BBa_K1465203
BBa_K1465203 Version 1 (Component)
Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from Synechococcus elongatus

BBa_K1968014
BBa_K1968014 Version 1 (Component)
Tcyc Phytobrick: cytochrome C gene transcriptional terminator from Saccharomyces cerevisiae

BBa_K1685002
BBa_K1685002 Version 1 (Component)
aeBlue with LVA tag and double terminator

BBa_K2172009
BBa_K2172009 Version 1 (Component)
Tac Promoter-RBS-GST-Thrombin Protease-GFP-Terminator

BBa_K542011
BBa_K542011 Version 1 (Component)
Catechol 2,3-dioxygenase with C-term Arg-tag (xylE-Arg)

BBa_K2123111
BBa_K2123111 Version 1 (Component)
Tac promoter with downstream mer operator + RFP (K081014)

BBa_K294205
BBa_K294205 Version 1 (Component)
This is a coding sequence of heat shock protein from E.coli

BBa_K2092004
BBa_K2092004 Version 1 (Component)
alcR (incl RBS), ethanol-activated transcription factor from A. nidulans

BBa_K1968009
BBa_K1968009 Version 1 (Component)
PglaA inducible promoter Phytobrick: glucoamylase gene promoter (PglaA) from Aspergillus niger

BBa_K1968013
BBa_K1968013 Version 1 (Component)
PgdaA constituve Phytobrick promoter: Glyceraldehyde-3-phosphate dehydrogenase from Aspergillus nige

BBa_K1974011
BBa_K1974011 Version 1 (Component)
T7 Promoter+RBS+Hv1a+linker+6X His-Tag

BBa_K1974013
BBa_K1974013 Version 1 (Component)
T7 Promoter+RBS+OAIP+linker+6X His-Tag

BBa_K809710
BBa_K809710 Version 1 (Component)
GAL promoter + kozak + ZIM17 + T7 RNAP + ADH1 terminator

BBa_K177035
BBa_K177035 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA) under control of RBS.3 (medium)

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

BBa_K1974022
BBa_K1974022 Version 1 (Component)
T7Promoter+RBS+Sf1a+linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1974021
BBa_K1974021 Version 1 (Component)
T7Promoter+RBS+Hv1a+linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1974023
BBa_K1974023 Version 1 (Component)
T7Promoter+RBS+OAIP+linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1036003
BBa_K1036003 Version 1 (Component)
lux pL controlled luxR with lux pR controlled gfp (LVA-tag)

BBa_K2123112
BBa_K2123112 Version 1 (Component)
Tac promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)

BBa_K1067000
BBa_K1067000 Version 1 (Component)
Periplasmic directed GFP SF with signal peptide TAT and RFP as background color

BBa_K1974033
BBa_K1974033 Version 1 (Component)
T7 Promoter+RBS+Hv1a+GS linker+snowdrop-lectin+linker+6X His-Tag

BBa_K2123115
BBa_K2123115 Version 1 (Component)
Universal promoter (Tac + JK26) for both growth phase with downstream mer operator + K081014

BBa_K2144011
BBa_K2144011 Version 1 (Component)
Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.