luxI
BBa_C0061 Version 1 (Component)
autoinducer synthetase for AHL

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_J23106
BBa_J23106 Version 1 (Component)
constitutive promoter family member

Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette

BBa_J61101
BBa_J61101 Version 1 (Component)
Ribosome Binding Site Family Member

BBa_I13453
BBa_I13453 Version 1 (Component)
Pbad promoter

BBa_B0011
BBa_B0011 Version 1 (Component)
LuxICDABEG (+/-)

pT7
BBa_I719005 Version 1 (Component)
T7 Promoter

mCh
BBa_J176005 Version 1 (Component)
mCh

BBa_B1001
BBa_B1001 Version 1 (Component)
Terminator (artifical, small, %T~=90)

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

BBa_B0014
BBa_B0014 Version 1 (Component)
double terminator (B0012-B0011)

BBa_J23119
BBa_J23119 Version 1 (Component)
constitutive promoter family member

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

pBad/araC
BBa_I0500 Version 1 (Component)
Inducible pBad/araC promoter

SEGA021
SEGA021 Version 1 (Component)
natural linear DNA.

SEGA022
SEGA022 Version 1 (Component)
natural linear DNA.

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

Scar 25
BBa_B0105 Version 1 (Component)
RFC 25 Scar Sequence

BBa_J23110
BBa_J23110 Version 1 (Component)
constitutive promoter family member

BBa_J61120
BBa_J61120 Version 1 (Component)
Ribosome Binding Site Family Member

BBa_I13507
BBa_I13507 Version 1 (Component)
Screening plasmid intermediate

BBa_J23101
BBa_J23101 Version 1 (Component)
constitutive promoter family member

BBa_J56012
BBa_J56012 Version 1 (Component)
Invertible sequence of dna includes Ptrc promoter

BBa_K1847014
BBa_K1847014 Version 1 (Component)
placUV5-lacO

GFP genera
BBa_E0840 Version 1 (Component)
GFP generator

cI+luxR
BBa_R0065 Version 1 (Component)
Promoter (lambda cI and luxR regulated -- hybrid)

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

BBa_I712074
BBa_I712074 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)

OmpR
BBa_R0082 Version 1 (Component)
Promoter (OmpR, positive)

LasR+PAI
BBa_R0079 Version 1 (Component)
Promoter (LasR & PAI regulated)

BBa_F2620
BBa_F2620 Version 1 (Component)
3OC₆HSL -> PoPS Receiver

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

pT7
BBa_K525998 Version 1 (Component)
Promoter T7 and RBS

hPCD
BBa_J176000 Version 1 (Component)
hPCD

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

BBa_I13504
BBa_I13504 Version 1 (Component)
Screening plasmid intermediate

BBa_I712004
BBa_I712004 Version 1 (Component)
CMV promoter

flyPCD
BBa_J176001 Version 1 (Component)
flyPCD

fshPCD
BBa_J176002 Version 1 (Component)
fshPCD

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

pBAD
BBa_K206000 Version 1 (Component)
pBAD strong

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

GFP report
BBa_E0240 Version 1 (Component)
GFP generator