BBa_K2044008
BBa_K2044008 Version 1 (Component)
Based on our project, <4,6> is the direct pathway from Site No.4 to Site No.6 in the map we design.

BBa_K2044003
BBa_K2044003 Version 1 (Component)
Based on our project, <2,3> is the direct pathway from Site No.2 to Site No.3 in the map we design.

BBa_K2044002
BBa_K2044002 Version 1 (Component)
Based on our project, <2,1> is the direct pathway from Site No.2 to Site No.1 in the map we design.

BBa_K2044005
BBa_K2044005 Version 1 (Component)
Based on our project, <2,6> is the direct pathway from Site No.2 to Site No.6 in the map we design.

BBa_K2044012
BBa_K2044012 Version 1 (Component)
Based on our project, <6,8> is the direct pathway from Site No.6 to Site No.8 in the map we design.

BBa_K2044011
BBa_K2044011 Version 1 (Component)
Based on our project,<6,4> is the direct pathway from Site No.6 to Site No.4 in the map we design.

BBa_K1695049
BBa_K1695049 Version 1 (Component)
pL8-UV5 + Riboswitch Bacteriophage 21 Codon Optimized Lysis Cassette S R Rz

BBa_K1033204
BBa_K1033204 Version 1 (Component)
pSBLb4E15 E. coli and lactobacilli shuttle vector with erythromycin resistance

BBa_K563053
BBa_K563053 Version 1 (Component)
vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

LacI
BBa_R0010 Version 1 (Component)
promoter (lacI regulated)

BBa_K802003
BBa_K802003 Version 1 (Component)
Shuttle vector for <i> E. coli</i> and <i>B. subtilis</i>

BBa_K1616003
BBa_K1616003 Version 1 (Component)
VVD link to YC155 (YFP Cter split)

BBa_K1616004
BBa_K1616004 Version 1 (Component)
VVD linked to YN155 (YFP Nter split)

BBa_J04795
BBa_J04795 Version 1 (Component)
Riboswitch designed to turn "ON" a protein

BBa_K1321362
BBa_K1321362 Version 1 (Component)
sfGFP fused to CBDcex driven by LacI

BBa_K936012
BBa_K936012 Version 1 (Component)
Leader sequence that brings protein to periplasm

BBa_I733004
BBa_I733004 Version 1 (Component)
Produce LacZ alpha in response to AHL

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

BBa_J36852
BBa_J36852 Version 1 (Component)
Streptavidin, single-chain dimer (no start codon)

BBa_K1615108
BBa_K1615108 Version 1 (Component)
mRFP fused to CBDclos driven by LacI promoter

BBa_I758600
BBa_I758600 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_K1657006
BBa_K1657006 Version 1 (Component)
It is called GAB. It have the resistance to glyphosate and glufosinate

[OriTR]+[R
BBa_K1439002 Version 1 (Component)
This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.

BBa_K1113411
BBa_K1113411 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome

BBa_K1412088
BBa_K1412088 Version 1 (Component)
A combination of theophylline aptamer and taRNA that can response theophylline to regulate circuit

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

lacI+pL
BBa_R0011 Version 1 (Component)
Promoter (lacI regulated, lambda pL hybrid)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_J23100
BBa_J23100 Version 1 (Component)
constitutive promoter family member

scar
BBa_J176041 Version 1 (Component)
Silver scar

lux pR
BBa_R0062 Version 1 (Component)
Promoter (luxR & HSL regulated -- lux pR)

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

BBa_J04450
BBa_J04450 Version 1 (Component)
RFP Coding Device

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

cI lam
BBa_R0051 Version 1 (Component)
promoter (lambda cI regulated)

luxr
BBa_C0062 Version 1 (Component)
luxR repressor/activator, (no LVA?)

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

P(Lac) IQ
BBa_I14032 Version 1 (Component)
promoter P(Lac) IQ

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)