IBMc369
IBMc369 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-77)-IDG-ER-LBD-S-M86(78-154)-S-ECF20(102-193)-T

IBMc371
IBMc371 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-85)-ELG-ER-LBD-S-M86(86-154)-S-ECF20(102-193)-T

IBMc381
IBMc381 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-159)-GAG-M86N-cat-Plux2-B32-M86C-S-mCherry*(160-236)-H6-T

IBMc408
IBMc408 Version 1 (Component)
pSEVA321-PBAD(SapI-)-B30-SrpR(1-109)-QGG-M86N-T

IBMc101
IBMc101 Version 1 (Component)
pSB3T5(empty)

IBMc374
IBMc374 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-119)-WDG-ER-LBD-S-M86(120-154)-S-ECF20(102-193)-T

IBMc065
IBMc065 Version 1 (Component)
pSB3T5(BsaI-)-PBAD-B30-mCherry*(1-159)-GAG-M86N-cat-Plux2-B32-M86C-S-mCherry*(160-236)-T

IBMc372
IBMc372 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-91)-HIG-ER-LBD-S-M86(92-154)-S-ECF20(102-193)-T

IBMc368
IBMc368 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-76)-TIG-ER-LBD-S-M86(77-154)-S-ECF20(102-193)-T

IBMc380
IBMc380 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-39)-LD-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(40-154)-S-mCherry*(193-236)-H6-T

BBa_K1367003
BBa_K1367003 Version 1 (Component)
This plasmid containing siRNA for LAR gene in Camelia sinensis, used to knockdown gene LAR.

BBa_K187000
BBa_K187000 Version 1 (Component)
pAB Universal Plasmid for BioBytes Gene Assembly System

BBa_K394000
BBa_K394000 Version 1 (Component)
Resistance Gene for G418 in Yeast

BBa_K1300009
BBa_K1300009 Version 1 (Component)
araC gene + pBAD + cI repressor

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_M31904
BBa_M31904 Version 1 (Component)
Gene 10 RBS and Promoter and ORF

BBa_K1189029
BBa_K1189029 Version 1 (Component)
TALE-A with a his tag linked to a K coil under the control of a LacI promoter

BBa_K2110006
BBa_K2110006 Version 1 (Component)
TPA regulatory gene expression system

BBa_K584008
BBa_K584008 Version 1 (Component)
Lambda cI and LuxR regulated hybrid promotor + RBS + MelA + RBS + AFP + term

BBa_K552001
BBa_K552001 Version 1 (Component)
CrtE Gene-Codon Optimized for Yeast

BBa_K1695030
BBa_K1695030 Version 1 (Component)
RBS + Bacteriophage λ codon-optimized S mutant gene

BBa_K316023
BBa_K316023 Version 1 (Component)
Chloraphenicol resistance gene with 5amyE

BBa_K195301
BBa_K195301 Version 1 (Component)
neu gene operon and terminator.

BBa_M45177
BBa_M45177 Version 1 (Component)
Pu gene for GTP cyclohydrolase 1

BBa_K1695027
BBa_K1695027 Version 1 (Component)
RBS + Bacteriophage λ S mutant gene

BBa_K1939003
BBa_K1939003 Version 1 (Component)
Mutant RBS with mCherry reporter gene

BBa_K1468007
BBa_K1468007 Version 1 (Component)
pT7 + gene encoding blue chromoprotein (BBa_K864401)

BBa_K1939000
BBa_K1939000 Version 1 (Component)
Mutant RBS with mCherry reporter gene

BBa_K1972006
BBa_K1972006 Version 1 (Component)
dszC (a monooxygenase gene ), E.coli-optimized

BBa_K1972004
BBa_K1972004 Version 1 (Component)
dszA (a monooxygenase gene ), E.coli-optimized

BBa_K1939004
BBa_K1939004 Version 1 (Component)
Mutant RBS with mCherry reporter gene

BBa_K1446003
BBa_K1446003 Version 1 (Component)
dcmR gene under ATC inducible control

BBa_M45077
BBa_M45077 Version 1 (Component)
Heavy Metal Binding Protein (hmbp) gene

BBa_J100184
BBa_J100184 Version 1 (Component)
Heat Induced Promoter on rpoD gene

BBa_K1939002
BBa_K1939002 Version 1 (Component)
Mutant RBS with mCherry reporter gene

BBa_J100199
BBa_J100199 Version 1 (Component)
Fitness Gene Tester + LVA degradation tag

BBa_K1939001
BBa_K1939001 Version 1 (Component)
Mutant RBS with mCherry reporter gene

BBa_K190074
BBa_K190074 Version 1 (Component)
Gas Vesicle polycistonic gene without repeat

BBa_K733003
BBa_K733003 Version 1 (Component)
RBS+<i>ydcD</i>: antitoxin gene

EpsE
BBa_K143032 Version 1 (Component)
EpsE Molecular Clutch Gene of B. subtilis

BBa_M31535
BBa_M31535 Version 1 (Component)
This is gene IX of M13K07 (RBS, and ORF)

BBa_M31534
BBa_M31534 Version 1 (Component)
This is gene VII of M13K07 (RBS, and ORF)

BBa_K195620
BBa_K195620 Version 1 (Component)
pRE with repressor gene TetR

BBa_M1209
BBa_M1209 Version 1 (Component)
Antiholin portion of kill gene

BBa_K2165001
BBa_K2165001 Version 1 (Component)
Violacein D gene codon-optimized for S. Cerevisiae

BBa_K2165000
BBa_K2165000 Version 1 (Component)
Violacein C gene codon-optimized for S. Cerevisiae

BBa_K861034
BBa_K861034 Version 1 (Component)
FadJ gene with a RBS and a terminator

P14,2
BBa_K1773006 Version 1 (Component)
pLac with RBS attached to cI gene.

ZEC
BBa_K1018001 Version 1 (Component)
she ble gene conferring Zeocin resistance

BBa_K195607
BBa_K195607 Version 1 (Component)
pPenI with activator gene CII