BBa_K587008
BBa_K587008 Version 1 (Component)
Crxst -> Lock sequence for low concentration mechanism

BBa_K648108
BBa_K648108 Version 1 (Component)
RecA without restriction sites or recombinant activity

BBa_K357002
BBa_K357002 Version 1 (Component)
muconolactone Delta-isomerase or muconolactone D-isomerase

BBa_J70405
BBa_J70405 Version 1 (Component)
R0010.J70399 (Ribosome Replacer or Tag Adder)

BBa_M30002
BBa_M30002 Version 1 (Component)
-- No description --

BBa_M30004
BBa_M30004 Version 1 (Component)
-- No description --

BBa_K116500
BBa_K116500 Version 1 (Component)
OmpF promoter that is activated or repressesed by OmpR according to osmolarity.

BBa_M30005
BBa_M30005 Version 1 (Component)
-- No description --

BBa_M30008
BBa_M30008 Version 1 (Component)
-- No description --

BBa_M30007
BBa_M30007 Version 1 (Component)
-- No description --

BBa_M30010
BBa_M30010 Version 1 (Component)
-- No description --

BBa_I735000
BBa_I735000 Version 1 (Component)
ech gene coding sequence for feruoyl CoA hydratese

BBa_K292009
BBa_K292009 Version 1 (Component)
Stem cell factor (SCF) or c-kit-ligand

BBa_K1363610
BBa_K1363610 Version 1 (Component)
key-1-of-or of RNA logic gates

BBa_K415069
BBa_K415069 Version 1 (Component)
pLux-Lac:GFP:Term:Term:PluxR/cI-OR :mCherry:Term:PtetR:LuxR:Term:Fixed-pLux/cI:LuxI

cln2
BBa_K105014 Version 1 (Component)
cln2 PEST destabilization domain for rapid protein turnover

BBa_K415007
BBa_K415007 Version 1 (Component)
pLux/cI-OR : RBS-mCherry : Term : pLux/cI-OR : RBS-luxI : Term : p(tetR) : RBS-luxR : Term

BBa_J24818
BBa_J24818 Version 1 (Component)
dLaRAP firefly luciferase reporter device for promoters

BBa_J70404
BBa_J70404 Version 1 (Component)
R0010.E0021.J70394.E0031 or J70398.E0031

BBa_J70403
BBa_J70403 Version 1 (Component)
R0010.E0031.J70394.E0021 or J70397.E0021

BBa_M11409
BBa_M11409 Version 1 (Component)
Promoter of pglnBP2 gene in Synechocystis sp. PCC 6803.

BBa_K1778002
BBa_K1778002 Version 1 (Component)
TRE-CYC1TATA is a recombinant promoter, which is constructed in order to make the Tet-on system func

BBa_M50016
BBa_M50016 Version 1 (Component)
Promoter, RBS and GolS Activator for Gold Detection

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K338062
BBa_K338062 Version 1 (Component)
GFP Reporter Device, Heat-Shock or IPTG Induced

BBa_K327017
BBa_K327017 Version 1 (Component)
lux OR delta cI activated "off" part

BBa_K812132
BBa_K812132 Version 1 (Component)
mCFP with kozak sequence for expression in Xenopus

BBa_K541000
BBa_K541000 Version 1 (Component)
Limulus anti-LPS factor (LALF) for Bacillus subtilis

BBa_K812133
BBa_K812133 Version 1 (Component)
sfGFP with kozak sequence for expression in Xenopus

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_K077025
BBa_K077025 Version 1 (Component)
HSL/LuxR or LasR promotor test system

BBa_J70368
BBa_J70368 Version 1 (Component)
I13502 or K093005 (B0034+E1010) rfp without PsrI sites

Holin, T4
BBa_K112003 Version 1 (Component)
T4 holin, without start or stop codon, berkeley standard

BBa_J329004
BBa_J329004 Version 1 (Component)
Quorum Sensing Sender Part for Use in BBa_J329003

BBa_K415004
BBa_K415004 Version 1 (Component)
P_const(high) : RBS : LuxR : Term : pLux/CI-OR

BBa_K1132038
BBa_K1132038 Version 1 (Component)
XOR-inverted RFP gate (BBa_K1132035) with T7 polymerase under the control of a strong promoter

BBa_M31748
BBa_M31748 Version 1 (Component)
Gene VII w/o Genes IX RBS or VIII Promoter

BBa_J70552
BBa_J70552 Version 1 (Component)
J70551.J70549 (or J70548.J70550.J70549) composite part

BBa_K750108
BBa_K750108 Version 1 (Component)
PcIGLT-pBADGLT:GFP(lva) expression system controlled by cI protein or arabinose

BBa_M36556
BBa_M36556 Version 1 (Component)
5' Bicistronic UTR (medium), does not include ATG start

BBa_K2123301
BBa_K2123301 Version 1 (Component)
Novel Synthetic Phytochelatin codon optimized for E. coli expression

KanR-BA-Bf
BBa_K349012 Version 1 (Component)
KanR-BA-BfuAI (for construction of BA BioBytes 2.0)

BBa_M31002
BBa_M31002 Version 1 (Component)
Refactored Gene 2 without Gene 10's Promoter or RBS

BBa_K1363611
BBa_K1363611 Version 1 (Component)
key-1 of gate-of-or of RNA logic gates

BBa_K360115
BBa_K360115 Version 1 (Component)
Mutated red luciferase based on BBa_I712019

BBa_K415003
BBa_K415003 Version 1 (Component)
P_const(low/med) : RBS : LuxR : Term : pLux/CI-OR

BBa_K112222
BBa_K112222 Version 1 (Component)
{< ihfB >} The ihf beta gene without start or stop codons, BBb format

BBa_M11407
BBa_M11407 Version 1 (Component)
Type 1 promoter of hspA gene in Synechocystis sp. PCC 6803