CGv2B
BBa_I732925 Version 1 (Component)
Combined Gates (version 2)

CGv2A
BBa_I732924 Version 1 (Component)
Combined Gates (version 1)

CGv2C
BBa_I732926 Version 1 (Component)
Combined Gates (version 3)

BBa_K180004
BBa_K180004 Version 1 (Component)
Game of Life - Primary plasmid

BBa_M36760
BBa_M36760 Version 1 (Component)
Channelrhodopsin-2 light-gated Ca2+ channel

BBa_J119141
BBa_J119141 Version 1 (Component)
Device for Testing New Promoters via BsgI Golden Gate Assembly

BBa_K1994017
BBa_K1994017 Version 1 (Component)
sgRNA with 5' golden gate adapter and PP7 protein binding site

BBa_K1994015
BBa_K1994015 Version 1 (Component)
sgRNA with 5' golden gate adapter and COM protein binding site

BBa_K1341011
BBa_K1341011 Version 1 (Component)
OR LOGIC GATE IN Graph Theory (GFP OUTPUT DEVICE)

BBa_J580003
BBa_J580003 Version 1 (Component)
Cate and Arnau testing the registry

BBa_J119313
BBa_J119313 Version 1 (Component)
pClone Blue - Device for Testing New Promoters via Golden Gate Assembly

BBa_J119137
BBa_J119137 Version 1 (Component)
pClone Red - Device for Testing New Promoters via Golden Gate Assembly

BBa_K1994016
BBa_K1994016 Version 1 (Component)
sgRNA with 5' golden gate adapter and MS2 coat protein binding site

BBa_M39023
BBa_M39023 Version 1 (Component)
AND gate structure for regulation of E.coli in CF mucus

BBa_K364120
BBa_K364120 Version 1 (Component)
Zinc Finger Protein GAT

BBa_J119367
BBa_J119367 Version 1 (Component)
tClone: Device for Testing Transciptional Terminators and Riboswitches via Golden Gate Assembly

BBa_K1649002
BBa_K1649002 Version 1 (Component)
Red light sensor with TetR not gate and GFP reporter

BBa_K812051
BBa_K812051 Version 1 (Component)
Golden brick version of pSB1K3 plasmid for Golden Gate and Golden Brick cloning

BBa_J119142
BBa_J119142 Version 1 (Component)
Part for inserting modified D-Dogs for Golden Gate Assembly using BsgI

BBa_J119361
BBa_J119361 Version 1 (Component)
tClone Red: Device for Testing Transciptional Terminators and Riboswitches via Golden Gate Assembly

pSB1C3 GB
BBa_K812050 Version 1 (Component)
Golden brick version of pSB1C3 plasmid for Golden Gate and Golden Brick cloning

BBa_K1363602
BBa_K1363602 Version 1 (Component)
key of yes of RNA logic gates

BBa_K090001
BBa_K090001 Version 1 (Component)
Constitutively expresses glutamate gated K+ channel

BBa_J119374
BBa_J119374 Version 1 (Component)
tClone TetA: Device for Testing Transciptional Terminators and Riboswitches via Golden Gate Assembly

BBa_K090002
BBa_K090002 Version 1 (Component)
Constitutively expresses glutamate gated K+ channel

BBa_K235025
BBa_K235025 Version 1 (Component)
[K235022][K235000] (NAND gate, pAra and pLac input signal control, mCherry output signal)

BBa_K1363610
BBa_K1363610 Version 1 (Component)
key-1-of-or of RNA logic gates

BBa_K2116028
BBa_K2116028 Version 1 (Component)
bxb1 integrase under the control of nitric oxide and AHL responsive AND gate

BBa_K363008
BBa_K363008 Version 1 (Component)
Cch1, voltage gated calcium channel from S. cerevisiae

BBa_K2116068
BBa_K2116068 Version 1 (Component)
AND gate regulated by norR and esaR (internal esabox, overlapping with sigma 54 binding site, before

BBa_K1132037
BBa_K1132037 Version 1 (Component)
AND-inverted RFP gate (BBa_K1132034) with T7 polymerase under the control of a strong promoter

BBa_K1132038
BBa_K1132038 Version 1 (Component)
XOR-inverted RFP gate (BBa_K1132035) with T7 polymerase under the control of a strong promoter

BBa_K077557
BBa_K077557 Version 1 (Component)
Part 1 of the Conways game of life system

BBa_K180007
BBa_K180007 Version 1 (Component)
Game of Life - Secondary plasmid [tac pR, LuxI generator]

BBa_K1903003
BBa_K1903003 Version 1 (Component)
Golden Gate Adapter

BBa_K363009
BBa_K363009 Version 1 (Component)
Mid1, votage and stretch gated calcium channel from S. cerevisiae

BBa_M41008
BBa_M41008 Version 1 (Component)
AND Gate Promoter

BBa_K2116004
BBa_K2116004 Version 1 (Component)
AND gate regulated by norR and esaR (one esabox)

LacIm
BBa_K092800 Version 1 (Component)
Coding sequence for LacI modified with a different rate of translation than LacI, RBS

BBa_K180006
BBa_K180006 Version 1 (Component)
Game of Life - Primary plasmid (part 2) [lux pR, GFP and LacI generator]

BBa_K228013
BBa_K228013 Version 1 (Component)
(pSal PO) OR Gate - GFP

BBa_J54141
BBa_J54141 Version 1 (Component)
AND GATE"AHL and oleate"

BBa_K2116006
BBa_K2116006 Version 1 (Component)
AND gate regulated by norR and esaR (three esaboxes)

BBa_K1363604
BBa_K1363604 Version 1 (Component)
key of no of RNA logic gates

BBa_J119300
BBa_J119300 Version 1 (Component)
Part for inserting modified D-Dogs for Golden Gate Assembly using BsgI

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.