BBa_M36027
BBa_M36027 Version 1 (Component)
phiX174 gene C - mutated rbs and start codon of D

FecR 1-85
BBa_K257020 Version 1 (Component)
FecR 1-85 - amplification of the ferric citrate induction response

BBa_K143003
BBa_K143003 Version 1 (Component)
5??? Integration Sequence for the PyrD locus of B. subtilis

PhlF
BBa_K1401004 Version 1 (Component)
MoClo Level 0 part of PhlF repressible gene. TetR homolog

BBa_K1216009
BBa_K1216009 Version 1 (Component)
Variant of the wild-type pLuxR promoter with lower sensitivity

BBa_K349004
BBa_K349004 Version 1 (Component)
RFP BbsI BA (for Construction of AB Type Bytes-v2)

BBa_K525010
BBa_K525010 Version 1 (Component)
Fusion Protein of S-Layer SbpA and LigA with His-Tag

BBa_K525009
BBa_K525009 Version 1 (Component)
Fusion Protein of S-Layer SgsE and LigA with His-Tag

BBa_M31540
BBa_M31540 Version 1 (Component)
Four Proteins involved in the production of the M13K07 phage coat.

BBa_K182001
BBa_K182001 Version 1 (Component)
Intermediate Part consisting of LacI ( RBS+, Term +) and a Tet operator.

iGEM 2019 Part Library
iGEM_2019_Parts Version 1 (Collection)
The iGEM Spring 2019 distribution of parts

iGEM 2018 Part Library
iGEM_2018_Parts Version 1 (Collection)
The iGEM Spring 2018 distribution of parts

BBa_K346034
BBa_K346034 Version 1 (Component)
T7p(BBa_I719005)+RBS(B0034)+Lpp-OmpA+MBP(surface display of mercury binding peptide)

BBa_K1675008
BBa_K1675008 Version 1 (Component)
A device used in the synthesis of lactic acid

BBa_K112222
BBa_K112222 Version 1 (Component)
{< ihfB >} The ihf beta gene without start or stop codons, BBb format

eYFP_GBD g
BBa_K1602040 Version 1 (Component)
Inducible generator of eYFP with C-terminal GBD ligand

BBa_K1707016
BBa_K1707016 Version 1 (Component)
TetR-ssRA repressor controlling the expression of CFP-ssRA

BBa_K1702005
BBa_K1702005 Version 1 (Component)
Trans-zeatin biosynthesis under control of the lac promoter

BBa_K1060014
BBa_K1060014 Version 1 (Component)
Strong constitutive expression of EBF synthase from Artemisia annua

BBa_K415003
BBa_K415003 Version 1 (Component)
P_const(low/med) : RBS : LuxR : Term : pLux/CI-OR

BBa_K557003
BBa_K557003 Version 1 (Component)
luxpR+cI: for modulating the ratio of Toggle switch

BBa_M1676
BBa_M1676 Version 1 (Component)
a construct to detect the presence/absence of glucose

Xylr senso
BBa_I723140 Version 1 (Component)
Xylene sensor for creation of reporter constructs (secondary version)

eYFP_PDZ g
BBa_K1602042 Version 1 (Component)
Inducible generator of eYFP with C-terminal PDZ ligand

BBa_K1104701
BBa_K1104701 Version 1 (Component)
The control group of positive feedback in suicide group

BBa_K1707029
BBa_K1707029 Version 1 (Component)
thermosensitive cI repressor controlling the expression of CFP-ssRA

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

BBa_K525002
BBa_K525002 Version 1 (Component)
Fusion protein of SbpA, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac

BBa_K525001
BBa_K525001 Version 1 (Component)
Fusion protein of SgsE, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac

GH371
BBa_V1011 Version 1 (Component)
E. coli strain GH371; 5-fluorouracil resistant upp mutant of GeneHogs

BBa_K1172910
BBa_K1172910 Version 1 (Component)
Part 2 of the Biosafety-System L with Barnase (dplac Barnase)

BBa_K222002
BBa_K222002 Version 1 (Component)
project of a Mid1 gene disrupted from 489 to 2021 nt

BBa_K883503
BBa_K883503 Version 1 (Component)
TuYV coat protein with part of the readthrough with His-tag

BBa_K1641006
BBa_K1641006 Version 1 (Component)
Fusion protein of Vcre::EGFP::ssra-tag, with RBS at beginning

BBa_J70000
BBa_J70000 Version 1 (Component)
sbpa (a protein that forms crystals in the presence of calcium)

BBa_K1641007
BBa_K1641007 Version 1 (Component)
Fusion protein of Scre::EGFP::ssra-tag, with RBS at beginning

BBa_K182100
BBa_K182100 Version 1 (Component)
Intermediate Part consisting of Lambda cI with Reporter Gene and double Terminators

BBa_K182002
BBa_K182002 Version 1 (Component)
Intermediate Part consisting of a cI regulated promoter and TetR (RBS+, Term-).

BBa_K180007
BBa_K180007 Version 1 (Component)
Game of Life - Secondary plasmid [tac pR, LuxI generator]

CBDclos(J6
BBa_K863114 Version 1 (Component)
CBD of C. cellulovorans cellulose binding protein gene with const. Promoter (J23100+J61101) and GFP

BBa_T9012
BBa_T9012 Version 1 (Component)
A mutant version of T9002 following a recombination event

BBa_I764000
BBa_I764000 Version 1 (Component)
production of tetR under the arabinose regulated promoter (AraC)

SAR
BBa_K2015000 Version 1 (Component)
Self-assembling region (SAR) for the circularization of proteins

BBa_J70556
BBa_J70556 Version 1 (Component)
BsaXI test circuit: J70555.J70554 or J70554.J70550.J70554

BBa_K078006
BBa_K078006 Version 1 (Component)
One part of the initial dioxygenase in dioxin degradation pathway

rbs+ftsK
BBa_I742164 Version 1 (Component)
ftsK (cell division protein) of E. coli with native rbs

BBa_J17000
BBa_J17000 Version 1 (Component)
G-alpha-olf

epsE 5 IS
BBa_K143005 Version 1 (Component)
5??? Integration Sequence for the epsE locus of B. subtilis

epsE 3 IS
BBa_K143006 Version 1 (Component)
3??? Integration Sequence for the epsE locus of B. subtilis

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.