BBa_J70664BBa_J70664 Version 1 (Component)Test assembly for Grant's parts
BBa_K2128100BBa_K2128100 Version 1 (Component)Strong promoter/RBS for HDLEA1
BBa_J07033BBa_J07033 Version 1 (Component)Composite part for FecA' (promoter::rbs::FecA')
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_J70302BBa_J70302 Version 1 (Component)Test plasmid for Mesoplasma florum
BBa_K174006BBa_K174006 Version 1 (Component)Sac single crossover site for Bacillus subtilis
BBa_K1437006BBa_K1437006 Version 1 (Component)mouse AhR detective protein 83-805 with terminator codon optimized for yeast
BBa_J72078BBa_J72078 Version 1 (Component){double terminator}, b0015 in BglBrick format
BBa_K187021BBa_K187021 Version 1 (Component)Kanamycin resistance gene in pBA, BioBytes plasmid
BBa_J70304BBa_J70304 Version 1 (Component)constitutive cat cassette for Mesoplasma florum
BBa_K541525BBa_K541525 Version 1 (Component)LALF for Bacillus subtilis with LipA signal peptide
BBa_R2111BBa_R2111 Version 1 (Component)Promoter with operator site for C2003
BBa_R2112BBa_R2112 Version 1 (Component)Promoter with operator site for C2003
BBa_R2113BBa_R2113 Version 1 (Component)Promoter with operator site for C2003
BBa_R2108BBa_R2108 Version 1 (Component)Promoter with operator site for C2003
BBa_R2110BBa_R2110 Version 1 (Component)Promoter with operator site for C2003
BBa_I732021BBa_I732021 Version 1 (Component)Template for Building Primer Family Member
BBa_J05221BBa_J05221 Version 1 (Component)Tripple Binding Site for R3-ATF6
BBa_I760001BBa_I760001 Version 1 (Component)ech protein generator for vanillin biosynthesis
BBa_K1781001BBa_K1781001 Version 1 (Component)HER2 (Human Epidermal Growth Factor 2) cytoplasmic domain
BBa_K390003BBa_K390003 Version 1 (Component)pilA secretion tag for Synechocystis sp. PCC 6803
BBa_K2184034BBa_K2184034 Version 1 (Component)SNP for non-taster NaCl &Sour- TAS1R1 rs34160967
BBa_K390004BBa_K390004 Version 1 (Component)art+ secretion tag for Synechocystis sp. PCC 6803
BBa_K1985010BBa_K1985010 Version 1 (Component)Sequence coding for amyloid Sup35 residues 1-61
BBa_K2092005BBa_K2092005 Version 1 (Component)alcR, ethanol-activated transcription factor from A. nidulans (incl. RBS)
Active ComBBa_K2054006 Version 1 (Component)All Oligos for simplified Tetrahedral Nanostructure
cI434 optBBa_K566011 Version 1 (Component)cI434 repressor from phage 434 optimized for E. coli cI (+LVA)
BBa_M1013BBa_M1013 Version 1 (Component)Complete Construct for Type I Phasin Secretion
BBa_M33350BBa_M33350 Version 1 (Component)RBS+CDS for TodS protein
BBa_M33354BBa_M33354 Version 1 (Component)RBS+CDS for TodT protein
BBa_K861174BBa_K861174 Version 1 (Component)A control device for BBa_K861169
BBa_K566041BBa_K566041 Version 1 (Component)Second State for Biphasic switch
BBa_I20254BBa_I20254 Version 1 (Component)Protein Generator for T7 gp5.3
BBa_K272001BBa_K272001 Version 1 (Component)Constitutive Expression Cassette for RFP
E coil ferBBa_K1189037 Version 1 (Component)His tag + Human ferritin di-subunit with E coil under LacI promoter
BBa_K1015012BBa_K1015012 Version 1 (Component)mhpA homology sequence(for RED recombination system)
ccdB_IndBBa_K1152007 Version 1 (Component)Helper construct for NRP-Indigoidine-tagging
LysisBBa_K836009 Version 1 (Component)Lysis device for R. opacus
Ori+KanBBa_K320004 Version 1 (Component)Origin for Asaia and resistance to Kanamycin
BBa_I733005BBa_I733005 Version 1 (Component)Produce GFP in presence of AHL
BBa_S03736BBa_S03736 Version 1 (Component)pLac-lox-RBS-Tet (in pSB1A2)
BBa_M45177BBa_M45177 Version 1 (Component)Pu gene for GTP cyclohydrolase 1
BBa_K1366001BBa_K1366001 Version 1 (Component)Genetic construct for lpp gen deletion
BBa_J61017BBa_J61017 Version 1 (Component)Biobrick converter for NotI-part-EcoRI
BBa_J61015BBa_J61015 Version 1 (Component)Biobrick converter for NotI-part-BamHI
BBa_J42000BBa_J42000 Version 1 (Component)Coding Region for Gentomycin Resistance GmR
BBa_J23004BBa_J23004 Version 1 (Component)Plasmid for destroying biobrick restriction sites
BBa_K1828002BBa_K1828002 Version 1 (Component)sgRNA for VicK under nlmAB promoter
ONBY RSBBa_K1416000 Version 1 (Component)The tRNA synthetase/tRNA needed for incorporating o-(2-nitrobenzyl)-L-tyrosine (ONBY) at a UAG codon
InsertionBBa_K1903021 Version 1 (Component)Insertion 2 for dCas9 version A