Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
BBa_B0034BBa_B0034 Version 1 (Component)RBS (Elowitz 1999) -- defines RBS efficiency
BBa_B0012BBa_B0012 Version 1 (Component)TE from coliphageT7
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
BBa_B0010BBa_B0010 Version 1 (Component)T1 from E. coli rrnB
BBa_B0015BBa_B0015 Version 1 (Component)double terminator (B0010-B0012)
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
p(tetR)BBa_R0040 Version 1 (Component)TetR repressible promoter
GFPBBa_E0040 Version 1 (Component)green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
BBa_B0030BBa_B0030 Version 1 (Component)RBS.1 (strong) -- modified from R. Weiss
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
BBa_B0031BBa_B0031 Version 1 (Component)RBS.2 (weak) -- derivative of BBa_0030
LacIBBa_R0010 Version 1 (Component)promoter (lacI regulated)
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
lacIBBa_C0012 Version 1 (Component)lacI repressor from E. coli (+LVA)
lacI+pLBBa_R0011 Version 1 (Component)Promoter (lacI regulated, lambda pL hybrid)
BBa_B0032BBa_B0032 Version 1 (Component)RBS.3 (medium) -- derivative of BBa_0030
BBa_B0033BBa_B0033 Version 1 (Component)RBS.4 (weaker) -- derivative of BBa_0030
mRFP1BBa_E1010 Version 1 (Component)**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
BBa_J23100BBa_J23100 Version 1 (Component)constitutive promoter family member
tetRBBa_C0040 Version 1 (Component)tetracycline repressor from transposon Tn10 (+LVA)
BBa_K1150042BBa_K1150042 Version 1 (Component)uniCAS RNaimer to Bla Target 4
BBa_K1150040BBa_K1150040 Version 1 (Component)uniCAS RNAimer to Bla Target 2
BBa_K1150038BBa_K1150038 Version 1 (Component)uniCAS RNAimer to target VEGF3
BBa_K1150037BBa_K1150037 Version 1 (Component)uniCAS RNaimer to target VEGF2
scarBBa_J176041 Version 1 (Component)Silver scar
lux pRBBa_R0062 Version 1 (Component)Promoter (luxR & HSL regulated -- lux pR)
BBa_K1150039BBa_K1150039 Version 1 (Component)uniCAS RNAimer to target VEGF4
BBa_K1150041BBa_K1150041 Version 1 (Component)uniCAS RNaimer to Bla Target 3
BBa_K1150043BBa_K1150043 Version 1 (Component)uniCAS RNAimer multiple targets to VEGF 2 and VEGF 3
BBa_J23119BBa_J23119 Version 1 (Component)constitutive promoter family member
BBa_K1150036BBa_K1150036 Version 1 (Component)uniCAS RNAimer to target VEGF1
BBa_K1150045BBa_K1150045 Version 1 (Component)uniCAS RNaimer multiple targets to VEGF 4 and bla target 2
cI lamBBa_R0051 Version 1 (Component)promoter (lambda cI regulated)
luxrBBa_C0062 Version 1 (Component)luxR repressor/activator, (no LVA?)
BBa_B1006BBa_B1006 Version 1 (Component)Terminator (artificial, large, %T~>90)
BBa_K1150046BBa_K1150046 Version 1 (Component)uniCAS RNaimer multiple targets to Bla target 2, 3, 4 and VEGF 4
BBa_K1150044BBa_K1150044 Version 1 (Component)uniCAS RNaimer multiple targets to Bla target 3 and 4
BBa_J23101BBa_J23101 Version 1 (Component)constitutive promoter family member
P(Lac) IQBBa_I14032 Version 1 (Component)promoter P(Lac) IQ
BBa_K2066018BBa_K2066018 Version 1 (Component)UNS 2 Sequence, from Torella et al., 2013