BBa_K784018
BBa_K784018 Version 1 (Component)
Phage Lambda fragment 4 - tail fiber proteins

BBa_K932001
BBa_K932001 Version 1 (Component)
EnvZ Protein

BBa_K784022
BBa_K784022 Version 1 (Component)
Phage Lambda fragment 8 - lytic proteins

BBa_K758000
BBa_K758000 Version 1 (Component)
UAS , this is the enhancer . And this enhancer is used to identify the protein localization .

BBa_K758002
BBa_K758002 Version 1 (Component)
GAL4, this protein binds the UAS

BBa_K763005
BBa_K763005 Version 1 (Component)
pADH2 + Gene encoding YAP1 protein

BBa_J72196
BBa_J72196 Version 1 (Component)
Payload Delivery Device

BBa_J72197
BBa_J72197 Version 1 (Component)
Payload Delivery Device

BBa_J72198
BBa_J72198 Version 1 (Component)
Payload Delivery Device with Barnase Payload

BBa_K758004
BBa_K758004 Version 1 (Component)
GAL4, this part coding protein binds and activates the UAS sequence

UAS-EGFP
BBa_K758006 Version 1 (Component)
UAS EGFP, this part expresses EGFP in the presence of GAL4 protein.

BBa_K812012
BBa_K812012 Version 1 (Component)
OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin

BBa_K812014
BBa_K812014 Version 1 (Component)
Auxin production device for expression in tadpole

BBa_K750000
BBa_K750000 Version 1 (Component)
pBADGLT:unstable gfp expression device activated by arabinose

BBa_K750001
BBa_K750001 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 1.0 strength)

BBa_K737016
BBa_K737016 Version 1 (Component)
This part conteins gvpA protein's coding sequence.

BBa_K737017
BBa_K737017 Version 1 (Component)
This part conteins gvpC-20psi protein

BBa_K737011
BBa_K737011 Version 1 (Component)
gvpC-GFP fusion protein with medium constitutive promoter

BBa_K737012
BBa_K737012 Version 1 (Component)
gvpC-GFP fusion protein UH with medium constitutive promoter

BBa_K737013
BBa_K737013 Version 1 (Component)
gvpC-GFP fusion protein

BBa_K737014
BBa_K737014 Version 1 (Component)
gvpC-GFP fusion protein UH

BBa_K750002
BBa_K750002 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.6 strength)

BBa_K750003
BBa_K750003 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.3 strength)

BBa_K750004
BBa_K750004 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.07 strength)

BBa_K750005
BBa_K750005 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.01 strength)

BBa_K750006
BBa_K750006 Version 1 (Component)
LuxR expression device activated by arabinose

CBDclos
BBa_K863111 Version 1 (Component)
Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)

BBa_K873000
BBa_K873000 Version 1 (Component)
heat shock 10kDa protein(hsp 10) groES coding resquence

BBa_K739004
BBa_K739004 Version 1 (Component)
Ax21 protein - signaling part

BBa_K737038
BBa_K737038 Version 1 (Component)
galK::GFP generator ligated to sRNA device controlled by IPTG

BBa_K737039
BBa_K737039 Version 1 (Component)
galK::GFP generator ligated to sRNA device controlled by aTc

BBa_K737040
BBa_K737040 Version 1 (Component)
galK::GFP generator ligated to TetR generator and constitutive sRNA device

BBa_K737041
BBa_K737041 Version 1 (Component)
galK::GFP generator ligated to LacI generator and constitutive sRNA device

pLac ColE2
BBa_K914001 Version 1 (Component)
pLac Colicin E2 Immunity protein

BBa_K914002
BBa_K914002 Version 1 (Component)
repressilator RBS Colicin E2 Immunity protein

BBa_K883160
BBa_K883160 Version 1 (Component)
CCMV_Negative coat protein

BBa_K883151
BBa_K883151 Version 1 (Component)
CCMV coat protein gene 25AA N-terminal replaced with HIStag under IPTG inducable promotor

gpS
BBa_K836006 Version 1 (Component)
Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)

BBa_K157010
BBa_K157010 Version 1 (Component)
glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

linker
BBa_K157013 Version 1 (Component)
15 aa flexible glycine-serine protein domain linker; Freiburg standard

BBa_K156009
BBa_K156009 Version 1 (Component)
OFP (orange fluorescent protein)

SBFP2
BBa_K156010 Version 1 (Component)
SBFP2 (strongly enhanced blue fluorescent protein)

BBa_I0466
BBa_I0466 Version 1 (Component)
RhlR Protein Generator

BBa_K156030
BBa_K156030 Version 1 (Component)
Genetic attenuator measurement device (template)

BBa_K116001
BBa_K116001 Version 1 (Component)
nhaA promoter, that can be regulated by pH and nhaR protein.

BBa_J04430
BBa_J04430 Version 1 (Component)
GFP coding device switched on by IPTG