ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Composite Sequence Pull
Composite Version 1 (Activity)
When multiple sources where combined to make a new part.

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

BBa_J61101
BBa_J61101 Version 1 (Component)
Ribosome Binding Site Family Member

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

SX scar
BBa_G0002 Version 1 (Component)
SpeI/XbaI mixed site

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

scar
BBa_G0000 Version 1 (Component)
SpeI/XbaI scar for RBS-CDS junctions

BBa_J61120
BBa_J61120 Version 1 (Component)
Ribosome Binding Site Family Member

Restriction_sites
Restriction_sites Version 1 (Component)
SacI - KpnI - SmaI - BamHI - XbaI - SalI - PstI - SphI - HindIII - NotI

scar
BBa_K300995 Version 1 (Component)
AvrII/XbaI mixed site (when XbaI comes from RFC10 Prefix) - rev

BBa_K300997
BBa_K300997 Version 1 (Component)
AvrII/XbaI mixed site (when XbaI comes from RFC10 Prefix)

RBS_cassette
RBS_cassette Version 1 (Component)
AvrII restriction site (cctagg), overlapped with RBS (aggagga)

BBa_K299107
BBa_K299107 Version 1 (Component)
T.xbaI.G (T followed by xbaI site and additional G to behave like biobrick prefix after xbaI digest)

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

AvrII/XbaI
BBa_B0104 Version 1 (Component)
AvrII/XbaI mixed site

scar
BBa_K300994 Version 1 (Component)
SpeI/AvrII mixed site (when SpeI comes from RFC10 Prefix) - rev

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

BBa_K300996
BBa_K300996 Version 1 (Component)
SpeI/AvrII mixed site (when SpeI comes from RFC10 Prefix)

scar
BBa_G0003 Version 1 (Component)
NheI/XbaI mixed site

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

BBa_J61100
BBa_J61100 Version 1 (Component)
Ribosome Binding Site Family Member

BBa_I712074
BBa_I712074 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

scar
BBa_G0001 Version 1 (Component)
XbaI/SpeI mixed site, reverse

BBa_J85600
BBa_J85600 Version 1 (Component)
attB1 recombination site

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

BBa_S00164
BBa_S00164 Version 1 (Component)
R0011 + B0100 + AvrII/XbaI scar

scar
BBa_G0006 Version 1 (Component)
XbaI/NheI mixed site

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

BBa_K299106
BBa_K299106 Version 1 (Component)
xbaI G (xbaI site and additional G to behave like biobrick prefix after xba digest)

AvrII
BBa_B0048 Version 1 (Component)
AvrII restriction enzyme site (XbaI, SpeI compatible overhang)