ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_K861500
BBa_K861500 Version 1 (Component)
FadL, Long-chain fatty acids transporter from <i>E.coli str. K12</i>

BBa_K861031
BBa_K861031 Version 1 (Component)
FadJ, gene for fatty acid degradation from <i>E.coli str. K12</i>

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_K861050
BBa_K861050 Version 1 (Component)
FadR, fatty acid sensor from <i>E.coli str. K12</i>

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

BBa_K861090
BBa_K861090 Version 1 (Component)
YhjH Gene From <i>E.coli str. K12</i>

BBa_K861041
BBa_K861041 Version 1 (Component)
FadI, gene for fatty acid degradation from <i>E.coli str. K12</i>

BBa_K861042
BBa_K861042 Version 1 (Component)
FadA, gene for fatty acid degradation from <i>Salmonella enterica</i> LT2

BBa_K861030
BBa_K861030 Version 1 (Component)
FadB, gene for fatty acid degradation from E.coli K12

BBa_I712074
BBa_I712074 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

phlF
BBa_K1725040 Version 1 (Component)
<i>phlF</i> encoding PhlF repressor from Pseudomonas protegens Pf-5

p_qrr4
BBa_K131017 Version 1 (Component)
p_qrr4 from <I>Vibrio harveyi</I>

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

BSMT1
BBa_J45004 Version 1 (Component)
SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

BBa_K733001
BBa_K733001 Version 1 (Component)
<i>Ptms</i> - a constitutive promoter in <i>E. coli</i> and <i>B. subtilis</i>.

BBa_K861021
BBa_K861021 Version 1 (Component)
FadE gene for fatty acid degradation from <i>Salmonella enterica</i> LT2

BBa_K733010
BBa_K733010 Version 1 (Component)
<i>Ptms</i>+RBS+<i>ydcD</i>.

BBa_K861032
BBa_K861032 Version 1 (Component)
S-FadB gene for fatty acid degradation from <i>Salmonella enterica</i> LT2

FadA
BBa_K861400 Version 1 (Component)
FadA protein coding sequence for fatty acid degradation

PfadR
BBa_K861060 Version 1 (Component)
PfadR, synthetic promoter with tandem FadR binding site

BBa_K531006
BBa_K531006 Version 1 (Component)
Optimized esp gene for <i>C. crescentus</i>

BBa_K733013
BBa_K733013 Version 1 (Component)
<i>Pveg</i>+R.B.S.

BBa_K733006
BBa_K733006 Version 1 (Component)
<i>ydjM</i>+<i>Bmp2</i>

BBa_K1638003
BBa_K1638003 Version 1 (Component)
T25 domain of <i>cyaA</i> from <i>Bordetella pertussis</i> (IPTG inducible)

BBa_K1315001
BBa_K1315001 Version 1 (Component)
The <i>pqsA</i> promoter from <i>Pseudomonas</i>

BBa_K1315008
BBa_K1315008 Version 1 (Component)
The <i>cblD</i> promoter from <i>Burkholderia</i>

BBa_K733012
BBa_K733012 Version 1 (Component)
<i>xylR</i>+<i>PxylA</i>+RBS+<i>ydcE</i>+<i>Ptms</i>+RBS+<i>ydcD</i>: Cell Growth Inhibition Device