Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

SEGA021
SEGA021 Version 1 (Component)
natural linear DNA.

SEGA022
SEGA022 Version 1 (Component)
natural linear DNA.

OmpR
BBa_R0082 Version 1 (Component)
Promoter (OmpR, positive)

Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

BBa_J44001
BBa_J44001 Version 1 (Component)
Reverse RBS (RBS_rev) -- corresponds to BBa_B0030

pT7
BBa_K525998 Version 1 (Component)
Promoter T7 and RBS

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

linker
BBa_K133132 Version 1 (Component)
8 aa protein domain linker

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

BBa_K1489002
BBa_K1489002 Version 1 (Component)
Lpp-OmpA-Linker (pSB1C3)

BBa_K214001
BBa_K214001 Version 1 (Component)
Pconst-RBS-Lpp-OmpA-Linker-TEV-TEV-Linker-ANP

BBa_K1321340
BBa_K1321340 Version 1 (Component)
Double CBD (dCBD) with N-terminal linker

OmpR
BBa_R0084 Version 1 (Component)
Promoter (OmpR, positive)

BBa_K1462502
BBa_K1462502 Version 1 (Component)
PDZ domain + Linker

BBa_K1150013
BBa_K1150013 Version 1 (Component)
AGS Linker

BBa_K1462503
BBa_K1462503 Version 1 (Component)
SH3 domain + Linker

BBa_K1462501
BBa_K1462501 Version 1 (Component)
GBD domian + Linker

BBa_K1321339
BBa_K1321339 Version 1 (Component)
CBDcenA+Linker, RFC 25 standard

BBa_K1638016
BBa_K1638016 Version 1 (Component)
8 aa linker without BamHI restriction site and TEV recognition site

BBa_K323107
BBa_K323107 Version 1 (Component)
PBSII zinc finger + linker

BBa_K195401
BBa_K195401 Version 1 (Component)
Lpp-OmpA-linker -F10scFv

linker
BBa_K157009 Version 1 (Component)
Split fluorophore linker; Freiburg standard

BBa_K215261
BBa_K215261 Version 1 (Component)
Lpp + 5tmr OmpA + GS Linker + TEV site + WT Streptavidin + 6His

RBS-GFP-Te
BBa_I763020 Version 1 (Component)
RBS-GFP (+LVA Tag)-Terminators

const.
BBa_R1075 Version 1 (Component)
Constitutive Promoter II

BBa_K2017006
BBa_K2017006 Version 1 (Component)
RSIAT-TEV linker + Luciferase

BBa_K215251
BBa_K215251 Version 1 (Component)
Lpp + 5tmr OmpA + GS Linker + TEV site + NheI Site

BBa_K215250
BBa_K215250 Version 1 (Component)
Lpp + 1tmr OmpA + GS Linker + TEV site + NheI Site

T7 RBS
BBa_K1362090 Version 1 (Component)
strong T7 RBS

OmpR
BBa_R0083 Version 1 (Component)
Promoter (OmpR, positive)

BBa_K215260
BBa_K215260 Version 1 (Component)
Lpp + 1tmr OmpA + GS Linker + TEV site + WT Streptavidin + 6His

RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS