ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

pdc
BBa_K273000 Version 1 (Component)
Pyruvate decarboxylase (pdc) from <i>Z. Mobilis</i> ATCC 29191

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_K273004
BBa_K273004 Version 1 (Component)
RBS - Pyruvate decarboxylase (pdc) from <i>Z. Mobilis</i> ATCC 29191

adh2
BBa_K273001 Version 1 (Component)
Alcohol dehydrogenase (adh2 , adhII, adhB) from <i>Z. Mobilis</i> ATCC 29191

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

pdc
BBa_K173016 Version 1 (Component)
pyruvate decarboxylase from Z. mobilis (codon optimized for E. coli)

BBa_I712074
BBa_I712074 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

pT7
BBa_K525998 Version 1 (Component)
Promoter T7 and RBS

RFC105 Z
BBa_K1362421 Version 1 (Component)
C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

BBa_K733010
BBa_K733010 Version 1 (Component)
<i>Ptms</i>+RBS+<i>ydcD</i>.

phlF
BBa_K1725040 Version 1 (Component)
<i>phlF</i> encoding PhlF repressor from Pseudomonas protegens Pf-5

p_qrr4
BBa_K131017 Version 1 (Component)
p_qrr4 from <I>Vibrio harveyi</I>

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

InvA
BBa_K901000 Version 1 (Component)
Invertase A from <i>Zymonas mobilis</i>

BBa_K733012
BBa_K733012 Version 1 (Component)
<i>xylR</i>+<i>PxylA</i>+RBS+<i>ydcE</i>+<i>Ptms</i>+RBS+<i>ydcD</i>: Cell Growth Inhibition Device

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

T7 RBS
BBa_K1362090 Version 1 (Component)
strong T7 RBS

BBa_K273017
BBa_K273017 Version 1 (Component)
RBS - Alcohol dehydrogenase (adh2 , adhII, adhB) from Z. Mobilis ATCC 29191

BBa_K733014
BBa_K733014 Version 1 (Component)
<i>Pveg</i>+RBS+<i>ydjM</i>+<i>Bmp2</i>

RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

BBa_K733018
BBa_K733018 Version 1 (Component)
<i>xylR</i>+<i>PxylA</i>+RBS+GFP+Double Terminator

BBa_K531006
BBa_K531006 Version 1 (Component)
Optimized esp gene for <i>C. crescentus</i>

BBa_K733013
BBa_K733013 Version 1 (Component)
<i>Pveg</i>+R.B.S.

BBa_K733006
BBa_K733006 Version 1 (Component)
<i>ydjM</i>+<i>Bmp2</i>

BBa_K733001
BBa_K733001 Version 1 (Component)
<i>Ptms</i> - a constitutive promoter in <i>E. coli</i> and <i>B. subtilis</i>.

BBa_K1638003
BBa_K1638003 Version 1 (Component)
T25 domain of <i>cyaA</i> from <i>Bordetella pertussis</i> (IPTG inducible)