BBa_K1963005
BBa_K1963005 Version 1 (Component)
A translational fusion between E. coli OsmY and E. coli Hfq

P43-spoVG
BBa_K143051 Version 1 (Component)
Promoter 43 and RBS spoVG for B. subtilis

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K197002
BBa_K197002 Version 1 (Component)
{OprF AtD}

BBa_K197007
BBa_K197007 Version 1 (Component)
{Hia AtD}

BBa_K197006
BBa_K197006 Version 1 (Component)
{Pcryo_1225 AtD}

BBa_K747102
BBa_K747102 Version 1 (Component)
pTAL-AID

BBa_K197001
BBa_K197001 Version 1 (Component)
{Azo1653 AtD}

BBa_K197005
BBa_K197005 Version 1 (Component)
{Hag AtD}

BBa_K541024
BBa_K541024 Version 1 (Component)
Reflectin 1A (SacB) and LALF (SacB) protein for B.Subtilis

BBa_K541027
BBa_K541027 Version 1 (Component)
Reflectin 1A (LipA) and LALF (LipA) protein for B.subtilis

BBa_K541025
BBa_K541025 Version 1 (Component)
Reflectin 1A (LipA) and LALF (SacB) protein for B.subtilis

BBa_M1000
BBa_M1000 Version 1 (Component)
Left mosaic end for Tn5 transpososome

BBa_K145105
BBa_K145105 Version 1 (Component)
cI under T7 and P_R dual promotor

BBa_I15007
BBa_I15007 Version 1 (Component)
ho1 and pcyA (PCB biosynthetic genes) ORFs from synechocystis

BBa_K541546
BBa_K541546 Version 1 (Component)
IPTG inducible PlacI, RBS,TAT signal peptide and reflectin

BBa_K1601008
BBa_K1601008 Version 1 (Component)
Linker between Laccase T. Thermophilus and CytC S. oneidensis

BBa_K1601011
BBa_K1601011 Version 1 (Component)
Linker between Laccase T. Thermophilus and CytC P. denitrificans

BBa_K1601012
BBa_K1601012 Version 1 (Component)
Linker between Laccase E. coli and CytC S. oneidensis

BBa_K1601009
BBa_K1601009 Version 1 (Component)
Linker between Laccase E. coli and CytC P. denitrificans

BBa_K1510930
BBa_K1510930 Version 1 (Component)
Constitutive promoter and RBS region for Streptococcus mutans transcription.

BBa_K1601013
BBa_K1601013 Version 1 (Component)
Linker between Laccase T. Thermophilus and CytC Synechocystis sp

BBa_K1601010
BBa_K1601010 Version 1 (Component)
Linker between Laccase E. coli and CytC Synechocystis sp

BBa_K1232001
BBa_K1232001 Version 1 (Component)
OmpR induced POmpC promoter and sfGFP = POmpC-RBS-sfGFP

BBa_K1894001
BBa_K1894001 Version 1 (Component)
shuttle plasmid which can replicate in cyanobacteria and E.coli

BBa_M31116
BBa_M31116 Version 1 (Component)
M13 Gene VIII terminator and promoter of Gene X

BBa_K1989044
BBa_K1989044 Version 1 (Component)
Triplespytag with mRFP, FlAsH-tag and signal peptide LipA

BBa_J64989
BBa_J64989 Version 1 (Component)
Lambda CI inbetween -35 and -10 of sigma 70

BBa_K1989042
BBa_K1989042 Version 1 (Component)
Triplespytag with mRFP, FlAsH-tag and signal peptide SacB

BBa_K1332011
BBa_K1332011 Version 1 (Component)
Histidine tag (8 AA) and RFP semi-permanent generator

BBa_K1989040
BBa_K1989040 Version 1 (Component)
Triplespytag with mRFP, FlAsH-tag and signal peptide ImdA

BBa_K1989043
BBa_K1989043 Version 1 (Component)
Triplespytag with mRFP, FlAsH-tag and signal peptide YjfA

AraC_TEV-F
BBa_K627010 Version 1 (Component)
Fusion of AraC induction system and TEV protease 3

BBa_K112801
BBa_K112801 Version 1 (Component)
{mRFP2}{mRFP2} Red Fluorescent Protein with start and Stop

FlipFlop
BBa_K907005 Version 1 (Component)
Dual phase protein generator(mRFP default). mRFP and GFP

BBa_K907004
BBa_K907004 Version 1 (Component)
Dual phase protein generator(GFP default). mRFP and GFP

BBa_K1989041
BBa_K1989041 Version 1 (Component)
Triplespytag with mRFP, FlAsH-tag and signal peptide NprE

BBa_M31532
BBa_M31532 Version 1 (Component)
This is gene X of M13K07 (Promoter, RBS, and ORF)

BBa_M31533
BBa_M31533 Version 1 (Component)
This is gene V of M13K07 (Promoter, RBS, and ORF)

BBa_M31531
BBa_M31531 Version 1 (Component)
This is gene II of M13K07 (Promoter, RBS, and ORF)

BBa_K1919003
BBa_K1919003 Version 1 (Component)
T7 promotor (under the control of lacI) and downstream CecropinXJ

PgsiB-spoV
BBa_K143061 Version 1 (Component)
Promoter gsiB and RBS spoVG for B. subtilis

Pctc-spoVG
BBa_K143059 Version 1 (Component)
Promoter ctc and RBS spoVG for B. subtilis

PgsiB-gsiB
BBa_K143060 Version 1 (Component)
Promoter gsiB and RBS gsiB for B. subtilis

Pctc-gsiB
BBa_K143058 Version 1 (Component)
Promoter ctc and RBS gsiB for B. subtilis

Pxyl-gsiB
BBa_K143056 Version 1 (Component)
Promoter xyl and RBS gsiB for B. subtilis

P43-gsiB
BBa_K143050 Version 1 (Component)
Promoter 43 and RBS gsiB for B. subtilis

BBa_I741020
BBa_I741020 Version 1 (Component)
promoter to xylF without CRP and several binding sites for xylR

BBa_I741021
BBa_I741021 Version 1 (Component)
promoter to xylA without CRP and several binding sites for xylR

BBa_K1919100
BBa_K1919100 Version 1 (Component)
The device consist of temperature promotor and hokD coding gene.