BBa_K1321004
BBa_K1321004 Version 1 (Component)
N-terminal rfc10_C-terminal rfc25 assembly

BBa_K359002
BBa_K359002 Version 1 (Component)
Agr quroum sensing sensor/generator, FepA pore, with P2 + reporter

BBa_K390062
BBa_K390062 Version 1 (Component)
GFP with art+ secretion tag for Synechocystis sp. PCC 6803

BBa_J33206
BBa_J33206 Version 1 (Component)
Bacillus subtilis ars promoter and arsR gene plus E. coli lacZ

BBa_K549016
BBa_K549016 Version 1 (Component)
E. coli ars promoter with arsR and ABS + GFP reporter gene

BBa_K549017
BBa_K549017 Version 1 (Component)
E. coli ars promoter with arsR and ABS + lacZ' reporter gene

DnaJ/GrpE
BBa_K205003 Version 1 (Component)
DnaJ and GrpE are chaperone proteins that assists with polypeptide folding

BBa_K549028
BBa_K549028 Version 1 (Component)
E. coli ars promoter with arsR and ABS + luxAB reporter gene

BBa_K748001
BBa_K748001 Version 1 (Component)
AgrC protein coding sequence. AgrC is main component of S.aureus agr quorum sensing system.

BBa_K844005
BBa_K844005 Version 1 (Component)
Spider Silk 1x 1E Subunit "U" with Met (ATG) start codon

BBa_K323005
BBa_K323005 Version 1 (Component)
CMV ATG PBSII link nYFP stop - part of mammalian split/FRET system

BBa_K323021
BBa_K323021 Version 1 (Component)
CMV ATG Gli1 link nCFP stop - part of mammalian split/FRET system

BBa_K1088016
BBa_K1088016 Version 1 (Component)
HRT2 prenyltransferase from Hevea Brasilianis (ara promoter with araC: arabinose inducible)

BBa_K1123006
BBa_K1123006 Version 1 (Component)
P(RG) Protein Production Construct

BBa_M10034
BBa_M10034 Version 1 (Component)
{˂Ag43_short!}, for Ag 43 autotransporter display system, only transporter

BBa_K874103
BBa_K874103 Version 1 (Component)
Arabinose inducible expression of PZF3838, M.ScaI Fusion protein (ARA -> PZF3838, M.ScaI FP)

BBa_K359005
BBa_K359005 Version 1 (Component)
Senses AIP and has RFP reporter (from Agr quorum sensing system)

ArsR Gen.
BBa_K935001 Version 1 (Component)
E. coli chromosomal ars promoter with arsR repressor gene and double terminator (B0010-B0012)

BBa_K323080
BBa_K323080 Version 1 (Component)
CMV ATG cYFP link Zif268 link His stop - part of mammalian split/FRET system

BBa_K323029
BBa_K323029 Version 1 (Component)
CMV ATG cCFP link ZNF HIVC His stop - part of mammalian split/FRET system

BBa_M45091
BBa_M45091 Version 1 (Component)
AGA1: Agglutinins, mating type specific cell surface Proteins, are synthesized by haploid cell of Sa

BBa_J70593
BBa_J70593 Version 1 (Component)
RFC12 ATG Head Domain

BBa_M31539
BBa_M31539 Version 1 (Component)
These are the genes in M13K07 responsible for forming the 'tip' of the phage.

BM-RFP
BBa_K774005 Version 1 (Component)
Bacterial-Mammalian promoter with RFP reporter: BBaK216005 + BHN + CArG promoter sequence E9-ns2 + B

BBa_K1051801
BBa_K1051801 Version 1 (Component)
Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib

BBa_K1362300
BBa_K1362300 Version 1 (Component)
NpuDnaE N-Intein RFC[105] assembly construct (with His6)

BBa_M36120
BBa_M36120 Version 1 (Component)
5' Bicistronic UTR (strong) contains ATG start Codon.

no ATG GFP
BBa_K579000 Version 1 (Component)
no ATG GFP

Plac/ara-1
BBa_K1145006 Version 1 (Component)
Drive the expression of LuxRI in pLuxRI2 and its derivatives.

BBa_J70498
BBa_J70498 Version 1 (Component)
Red {-1,4;0,5} PsrI part (for non protein/AG parts)

BBa_K1961000
BBa_K1961000 Version 1 (Component)
CYP2A13 is a member of the Cytochrome P450 (CYP) enzymes family, which are critical for the metaboli

BBa_M10035
BBa_M10035 Version 1 (Component)
{˂Ag43_long!} for Ag 43 autotransporter display system, including passenger domain

BBa_K329043
BBa_K329043 Version 1 (Component)
Right arm Tn916 - Mutant + Mutant Lambda

BBa_J58009
BBa_J58009 Version 1 (Component)
Fusion protein Trg-EnvZ

BBa_I716015
BBa_I716015 Version 1 (Component)
RFP without start ATG

BBa_K323075
BBa_K323075 Version 1 (Component)
ATG cYFP link HIVC

BBa_K329042
BBa_K329042 Version 1 (Component)
Right arm Tn916 - Wild Type + Mutant Lambda

BBa_I720010
BBa_I720010 Version 1 (Component)
Ara landing pad (pBBLP 8)

BBa_K299800
BBa_K299800 Version 1 (Component)
standard biobrick scar (if next part starts with ATG)

BBa_K1447004
BBa_K1447004 Version 1 (Component)
Epitope 1-5 from Ara h 1

BBa_K1447005
BBa_K1447005 Version 1 (Component)
Epitope 1-5 from Ara h 1

BBa_M36556
BBa_M36556 Version 1 (Component)
5' Bicistronic UTR (medium), does not include ATG start

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.