RADBBa_K651001 Version 1 (Component)RecA promoter with RFP coding device
BBa_K581001BBa_K581001 Version 1 (Component)ptsG1-GFP (ptsG1 5' UTR fused with gfp)
BBa_K581004BBa_K581004 Version 1 (Component)ptsG2-GFP (ptsG2 5'UTR fused with gfp)
BBa_K581006BBa_K581006 Version 1 (Component)Pc+ptsG(wt)-GFP (ptsG(wt) 5' UTR fused with gfp)
BBa_K633000BBa_K633000 Version 1 (Component)CelD Mutated Cellulase
037RPMGFPTBBa_K733007 Version 1 (Component)<i>Pveg</i> + spoVG RBS + <i>lytC</i> + linker + RPMrel + consensus RBS + GFP + double terminator
BBa_J100097BBa_J100097 Version 1 (Component)Anhydrotetracycline inducible promoter with BsaI sticky ends
BBa_K764005BBa_K764005 Version 1 (Component)WbbC with point mutation
BBa_K748006BBa_K748006 Version 1 (Component)S.aureus killing device. Truncated lysostaphin coding sequence with IPTG inducible promoter.
BBa_K748007BBa_K748007 Version 1 (Component)Biofilm formation device. yddV gene with the IPTG inducible promoter.
BBa_K764012BBa_K764012 Version 1 (Component)WbbC with point mutation + BBa_B0014
CMV AID pABBa_K929000 Version 1 (Component)AID with CMV promoter and hGH-polyadenylation signal sequence
BBa_K929001BBa_K929001 Version 1 (Component)AID without NES, with NLS and Kozak sequence
BBa_K733002BBa_K733002 Version 1 (Component)<i>xylR</i>+<i>PxylA</i>: A xylose inducible promoter with its transcriptional regulator.
BBa_K821001BBa_K821001 Version 1 (Component)RFP Reporter with o-RBS
BBa_K883201BBa_K883201 Version 1 (Component)Hepatitits B core antigen with IPTG induced promotor
BBa_J72198BBa_J72198 Version 1 (Component)Payload Delivery Device with Barnase Payload
BBa_J72208BBa_J72208 Version 1 (Component)Kanamycin resistant BAC with R6K origin
BBa_J72210BBa_J72210 Version 1 (Component)Kanamycin resistant BAC with R6K origin, pGlpT 5' of cloning site
BBa_J72211BBa_J72211 Version 1 (Component)ampicillin resistant p15A vector with dapD
BBa_K737000BBa_K737000 Version 1 (Component)We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig
BBa_K737001BBa_K737001 Version 1 (Component)We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig
BBa_K812001BBa_K812001 Version 1 (Component)Biobricked pSC2+ plasmid with pElastase promoter for frog use
GFP-AIDBBa_K812010 Version 1 (Component)GFP fused with AID-tag (ubuquitinase E3 OsTirI recoginition domain)
BBa_K817017BBa_K817017 Version 1 (Component)pSB2K3 with parS site
BBa_K929002BBa_K929002 Version 1 (Component)modified AID with CMV and hGH-polyA
BBa_K929003BBa_K929003 Version 1 (Component)modified AID with CMV, hGH-polyA and eGFP
BBa_K737011BBa_K737011 Version 1 (Component)gvpC-GFP fusion protein with medium constitutive promoter
BBa_K737021BBa_K737021 Version 1 (Component)gvpC-20 coding gene with GFP mut 3.1 fusion gene
BBa_K737012BBa_K737012 Version 1 (Component)gvpC-GFP fusion protein UH with medium constitutive promoter
BBa_K737022BBa_K737022 Version 1 (Component)This part is gvpC-20 coding gene with GFP mut 3.1 fusion gene
BBa_K863005BBa_K863005 Version 1 (Component)ecol laccase from E. coli with T7 promoter, RBS and His-tag
BBa_K863000BBa_K863000 Version 1 (Component)bpul (laccase from Bacillus pumilus) with T7 promoter, RBS and HIS tag
BBa_K863010BBa_K863010 Version 1 (Component)tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag
BBa_K861071BBa_K861071 Version 1 (Component)AdrA gene with a RBS and a terminator
BBa_K737066BBa_K737066 Version 1 (Component)E0040 without AUG
BBa_K861091BBa_K861091 Version 1 (Component)YhjH gene with a RBS and a Terminator
BBa_K863012BBa_K863012 Version 1 (Component)tthl laccase from T. thermophilus with constitutive promoter J23100, RBS and His-tag
sboA-AmilCBBa_K818400 Version 1 (Component)AmilCP with sboA regulator
BBa_K818500BBa_K818500 Version 1 (Component)Lycopene with sboA regulator
sboA-AmilGBBa_K818600 Version 1 (Component)AmilGFP with sboA regulator
BBa_K863020BBa_K863020 Version 1 (Component)bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag
BM-CFPBBa_K774004 Version 1 (Component)Bacterial-Mammalian promoter with eCFP reporter: BBaK216005 + CArG promoter sequence + BBa_E0420
BM-RFPBBa_K774005 Version 1 (Component)Bacterial-Mammalian promoter with RFP reporter: BBaK216005 + BHN + CArG promoter sequence E9-ns2 + B
MB-CFPBBa_K774006 Version 1 (Component)Mammalian-Bacterial promoter with eCFP reporter: CArG promoter sequence + BBaK216005 + BBa_E0420
MB-RFPBBa_K774007 Version 1 (Component)Mammalian-Bacterial promoter with RFP reporter: CArG promoter sequence + BBaK216005 + BBa_K08101
BBa_K883151BBa_K883151 Version 1 (Component)CCMV coat protein gene 25AA N-terminal replaced with HIStag under IPTG inducable promotor
CBDcex(T7)BBa_K863102 Version 1 (Component)Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.