BBa_K187090
BBa_K187090 Version 1 (Component)
terminator forward strand B, Biobytes assembly method

BBa_K187074
BBa_K187074 Version 1 (Component)
anchor forward strand B, Biobytes assembly method

BBa_J64713
BBa_J64713 Version 1 (Component)
LasR/LasI Inducible (RHLR/RHLI repressible) positive feedback production of 3OC12 HSL

NarL
BBa_M33030 Version 1 (Component)
NarL: Regulates nitrate reduction (strong RBS)

BBa_M33033
BBa_M33033 Version 1 (Component)
NarL: regulates nitrate reduction (weak RBS)

BBa_J64714
BBa_J64714 Version 1 (Component)
RHLR/RHLI Inducible (LasR/LasI repressible) positive feedback production of C4 HSL

BBa_K1407003
BBa_K1407003 Version 1 (Component)
constitutive production of LasR +LasR/PAI1 Inducible promoter+RBS(B0030)+RFP

BBa_K1407006
BBa_K1407006 Version 1 (Component)
constitutive production of LasR + LasR/PAI1 Inducible promoter +RBS(B0034)+GFP

BBa_J24812
BBa_J24812 Version 1 (Component)
Heavy Metal Translocating P-type ATPase

BBa_M45077
BBa_M45077 Version 1 (Component)
Heavy Metal Binding Protein (hmbp) gene

BBa_K187423
BBa_K187423 Version 1 (Component)
Anchor forward strand A for Biobytes assembly method

BBa_K1478002
BBa_K1478002 Version 1 (Component)
Top metal binding protein. Binds zinc, arsenic, copper and cadmium.

BBa_K1587009
BBa_K1587009 Version 1 (Component)
P_ompC and lacI box regulated cI and formate production

BBa_K1995025
BBa_K1995025 Version 1 (Component)
this part can bind heavy metal ions

BBa_I8035
BBa_I8035 Version 1 (Component)
Booperator aiia test 6: Effect of tetR on production of LuxR

BBa_I8033
BBa_I8033 Version 1 (Component)
Booperator aiia test 4: effect of tetR on production of RhlR

BBa_I731014
BBa_I731014 Version 1 (Component)
The luxR based receiver, F2620 (formerly I13270), controls the production of mCherry

BBa_I8032
BBa_I8032 Version 1 (Component)
Booperator aiia test 3: effect of tetR on production of RhlR

BBa_I8034
BBa_I8034 Version 1 (Component)
Booperator aiia test 5: effect of tetR on production of LuxR

BBa_K322922
BBa_K322922 Version 1 (Component)
cat + sacB for BRIDGE: A biobrick compatible markerless recombineering method

BBa_K1407008
BBa_K1407008 Version 1 (Component)
constitutive production of LasR+LasR/PAI1 Inducible promoter +RBS(B0034)+GFP+Terminator

BBa_K1172302
BBa_K1172302 Version 1 (Component)
ribC: 6,7-dimethyl-8-ribityllumazine synthase alpha subunit RibC

BBa_K2141002
BBa_K2141002 Version 1 (Component)
ECFP-meth scFv-EYFP

BBa_K346003
BBa_K346003 Version 1 (Component)
RBS(B0032)+MBP(mercury metal binding peptide engineered from MerR)

BBa_K400000
BBa_K400000 Version 1 (Component)
composite heavy metal-detector(Zn, Cd, As)

BBa_K346032
BBa_K346032 Version 1 (Component)
T7promoter(I719005)_RBS(B0034)_MBP(mercury metal binding peptide)

BBa_K129004
BBa_K129004 Version 1 (Component)
LamB_HP-metal binding domain in LamB-153 residue

BBa_K129005
BBa_K129005 Version 1 (Component)
LamB_CP-metal binding domain in LamB-153 residue

BBa_K220001
BBa_K220001 Version 1 (Component)
Glycine Sarcosine Methyl Transferase Gene

BBa_K2176001
BBa_K2176001 Version 1 (Component)
Cassette for the constitutive production of GAL4-KaiCp and LexA-SasA, linked by a self-cleaving P2A

BBa_K394004
BBa_K394004 Version 1 (Component)
An GAL1 Inducible Sex-Lethal Construct with a His tag

BBa_K2141003
BBa_K2141003 Version 1 (Component)
yfp-bicistronic anti-meth antibody-cfp

BBa_K258012
BBa_K258012 Version 1 (Component)
AI2-dependent KGF synthesis with RFP reporter and AHL production for Quaroum Sensin Death Mechanism

BBa_K346031
BBa_K346031 Version 1 (Component)
T7p(BBa_I719005)+RBS(B0034)+MBP(lead metal binding peptide)

BBa_M45134
BBa_M45134 Version 1 (Component)
Uranium reduction from VI valent state to IV valent state

BBa_K175035
BBa_K175035 Version 1 (Component)
Constitutive expression of GFP with medium RBS lock and inducible production of key for the lock

BBa_K175034
BBa_K175034 Version 1 (Component)
Constitutive expression of GFP with weak RBS lock and inducible production of key for the lock

BBa_K750115
BBa_K750115 Version 1 (Component)
pBADcIT-PtetcIT-PcIGLT: arabinose or atc->cI protein->reduction of gfp(lva)

MtrC
BBa_K1748003 Version 1 (Component)
Extracelllular metal reducing respiratory system periplasmic decaheme cytochrome C component MtrC

OmcA
BBa_K1748002 Version 1 (Component)
Extracelllular metal reducing respiratory system periplasmic decaheme Cytochrome C component OmcA

MtrA
BBa_K1748000 Version 1 (Component)
Extracelllular metal reducing respiratory system periplasmic decaheme cytochrome C component MtrA

BBa_K176247
BBa_K176247 Version 1 (Component)
High input score automatic selector without lethal device (with reporter): PoPS->mRFP->K176240->PoPS

BBa_K1884009
BBa_K1884009 Version 1 (Component)
PSAD Promoter+GalAD-CRY2+UAS+FNR(fumarate and nitrate reduction)+PSAD Terminator

MtrB
BBa_K1748001 Version 1 (Component)
Extracelllular metal reducing respiratory system integral outer-membrane β-barrel protein MtrB

BBa_K1655001
BBa_K1655001 Version 1 (Component)
This GFP can be fused into any protein's aminoterminal end with the BioBrick enzyme assembly method.

BBa_K176249
BBa_K176249 Version 1 (Component)
Low input score automatic selector w/o lethal device(with reporter): PoPS->mRFP->CI-LVA+pCI->K176240

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.