BBa_K1362300
BBa_K1362300 Version 1 (Component)
NpuDnaE N-Intein RFC[105] assembly construct (with His6)

BBa_K1972014
BBa_K1972014 Version 1 (Component)
dszBBAC with tac promoter and dszD with lac promotor induced by IPTG

CspB | RFP
BBa_K525234 Version 1 (Component)
Fusion Protein of mRFP, S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7, RBS

BBa_K525264
BBa_K525264 Version 1 (Component)
Fusion Protein of Luciferase, S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7

BBa_K323080
BBa_K323080 Version 1 (Component)
CMV ATG cYFP link Zif268 link His stop - part of mammalian split/FRET system

BBa_K323029
BBa_K323029 Version 1 (Component)
CMV ATG cCFP link ZNF HIVC His stop - part of mammalian split/FRET system

BBa_K525261
BBa_K525261 Version 1 (Component)
Fusion Protein of Luciferase and cspB from Corynebacterium halotolerans with TAT-Sequence and lipid

cspB
BBa_K525221 Version 1 (Component)
S-layer cspB from Corynebacterium halotolerans with TAT-Sequence and lipid anchor, PT7 and RBS

cspB
BBa_K525121 Version 1 (Component)
S-layer cspB from Corynebacterium glutamicum with TAT-Sequence and lipid anchor, PT7 and RBS

BBa_K1685002
BBa_K1685002 Version 1 (Component)
aeBlue with LVA tag and double terminator

BBa_K1932007
BBa_K1932007 Version 1 (Component)
This device is constructed for the expression of TAT-apoptin fused with tmp1.

BBa_K525161
BBa_K525161 Version 1 (Component)
Fusion Protein of Luciferase and cspB from Corynebacterium glutamicum with TAT-Sequence and lipid an

BBa_J70593
BBa_J70593 Version 1 (Component)
RFC12 ATG Head Domain

BBa_K1441013
BBa_K1441013 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag INSERT

BBa_K1051801
BBa_K1051801 Version 1 (Component)
Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib

BBa_K542011
BBa_K542011 Version 1 (Component)
Catechol 2,3-dioxygenase with C-term Arg-tag (xylE-Arg)

BBa_M36120
BBa_M36120 Version 1 (Component)
5' Bicistronic UTR (strong) contains ATG start Codon.

BBa_K1974011
BBa_K1974011 Version 1 (Component)
T7 Promoter+RBS+Hv1a+linker+6X His-Tag

BBa_K1974013
BBa_K1974013 Version 1 (Component)
T7 Promoter+RBS+OAIP+linker+6X His-Tag

BBa_K1657002
BBa_K1657002 Version 1 (Component)
It is called TAB. It have the resistance to glyphosate and the 2, 4-Dichlorophenoxyacetic acid

BBa_K1657004
BBa_K1657004 Version 1 (Component)
It is called TAB. It have the resistance to glyphosate and the 2, 4-Dichlorophenoxyacetic acid

Co-op
BBa_K233002 Version 1 (Component)
Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway

BBa_K233001
BBa_K233001 Version 1 (Component)
Snowdrift 1 , using TorA. A module to secrete B-galactosidase using the TAT-export Pathway

Hax3-2xNG
BBa_K2075023 Version 1 (Component)
TAL-effector Hax3-2xNG

BBa_K1321362
BBa_K1321362 Version 1 (Component)
sfGFP fused to CBDcex driven by LacI

BBa_K1974022
BBa_K1974022 Version 1 (Component)
T7Promoter+RBS+Sf1a+linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1974021
BBa_K1974021 Version 1 (Component)
T7Promoter+RBS+Hv1a+linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1974023
BBa_K1974023 Version 1 (Component)
T7Promoter+RBS+OAIP+linker+snowdrop-lectin+linker+6X His-Tag

BBa_I716015
BBa_I716015 Version 1 (Component)
RFP without start ATG

BBa_K1036003
BBa_K1036003 Version 1 (Component)
lux pL controlled luxR with lux pR controlled gfp (LVA-tag)

BBa_K1441012
BBa_K1441012 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

BBa_K1615108
BBa_K1615108 Version 1 (Component)
mRFP fused to CBDclos driven by LacI promoter

BBa_K323075
BBa_K323075 Version 1 (Component)
ATG cYFP link HIVC

BBa_K1974033
BBa_K1974033 Version 1 (Component)
T7 Promoter+RBS+Hv1a+GS linker+snowdrop-lectin+linker+6X His-Tag

BBa_K299800
BBa_K299800 Version 1 (Component)
standard biobrick scar (if next part starts with ATG)

BBa_K2144011
BBa_K2144011 Version 1 (Component)
Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter

BBa_K1106013
BBa_K1106013 Version 1 (Component)
pag activator from PSP3 phage generator

BBa_K726009
BBa_K726009 Version 1 (Component)
T7 driven lac operated inducer for the rhl quorum-sensing system

BBa_K2172009
BBa_K2172009 Version 1 (Component)
Tac Promoter-RBS-GST-Thrombin Protease-GFP-Terminator

BBa_M36556
BBa_M36556 Version 1 (Component)
5' Bicistronic UTR (medium), does not include ATG start

BBa_K2123112
BBa_K2123112 Version 1 (Component)
Tac promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)

BBa_K1067000
BBa_K1067000 Version 1 (Component)
Periplasmic directed GFP SF with signal peptide TAT and RFP as background color

BBa_K2123115
BBa_K2123115 Version 1 (Component)
Universal promoter (Tac + JK26) for both growth phase with downstream mer operator + K081014

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.