BBa_K1362061
BBa_K1362061 Version 1 (Component)
N-terminal chitin binding domain with triglycine linker and His-tag as RFC[105] insert

xis
BBa_K112204 Version 1 (Component)
{a~xis!} The bacteriophage lambda xis gene ready to have rbs attached and stop codon; assembly stand

BBa_K325109
BBa_K325109 Version 1 (Component)
EPIC Firefly Luciferase and LRE (under pBAD)<BR><i>P. Pyralis<BR>(E. coli optimised)</i>

BBa_K2082237
BBa_K2082237 Version 1 (Component)
RFP and Tetracycline resistence(tetA) under the control of a modified lacZ promoter; Fusion protein

BBa_K1113501
BBa_K1113501 Version 1 (Component)
GFP with a targeting sequence for delivery into the Carboxysome and a ssrA degradation tag

BBa_K079049
BBa_K079049 Version 1 (Component)
GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator

Altered Sequence Activities
AlteredSequenceActivities_collection Version 1 (Collection)
A set of activities to point to ways in which sequences have been altered between source and upload

BBa_K079020
BBa_K079020 Version 1 (Component)
GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein

BBa_K531010
BBa_K531010 Version 1 (Component)
Constitutive promoter and RBS BBa_K081005 + <i>Caulobacter</i> optimized <i>dspB</i> + <i>rsaA</i> C

BBa_M11207
BBa_M11207 Version 1 (Component)
Device containing HXT7/mNarK promoters and GFP/RFP to monitor glucose/oxygen levels in S. cerevisiae

BBa_M11208
BBa_M11208 Version 1 (Component)
Device containing HXT1/mNarK promoters and GFP/RFP to monitor glucose/oxygen levels in S. cerevisiae

BBa_M1530
BBa_M1530 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag with secretion tag and GFP

BBa_M1515
BBa_M1515 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP

BBa_M1529
BBa_M1529 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP

BBa_M1541
BBa_M1541 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP

BBa_K258012
BBa_K258012 Version 1 (Component)
AI2-dependent KGF synthesis with RFP reporter and AHL production for Quaroum Sensin Death Mechanism

BBa_M1531
BBa_M1531 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP

BBa_K2173000
BBa_K2173000 Version 1 (Component)
aiiA with degradation tag expressed under Plux-lambda hybrid promoter and a double terminator + mRFP

BBa_M1542
BBa_M1542 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP

BBa_M1535
BBa_M1535 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP

BBa_M1536
BBa_M1536 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag with secretion tag and GFP

BBa_K2116040
BBa_K2116040 Version 1 (Component)
AND gate 105

BBa_K2116039
BBa_K2116039 Version 1 (Component)
AND gate 104

BBa_I733007
BBa_I733007 Version 1 (Component)
Weight cells either turn blue or die in response to both inputs and HSL level

BBa_I3420
BBa_I3420 Version 1 (Component)
Test: Switch functionality and bistability (I3410.E0422.I3411.E0432.I3100.I3400.I3101.I3400.I3102.I3

BBa_K175035
BBa_K175035 Version 1 (Component)
Constitutive expression of GFP with medium RBS lock and inducible production of key for the lock

BBa_K175034
BBa_K175034 Version 1 (Component)
Constitutive expression of GFP with weak RBS lock and inducible production of key for the lock

BBa_M41008
BBa_M41008 Version 1 (Component)
AND Gate Promoter

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.

BBa_K887004
BBa_K887004 Version 1 (Component)
Plac+alsS+ilvC+ilvD(each preceded by own zinc-finger and RBS)+Ptet+B0032+kivD+B0015

BBa_M45111
BBa_M45111 Version 1 (Component)
RBS and Phasin

BBa_I13035
BBa_I13035 Version 1 (Component)
3OC₆HSL Receiver Device with Inducible Control of LuxR and a YFP Output device

BBa_J119316
BBa_J119316 Version 1 (Component)
Scaffold 2.0 for J-GGA (With the promoter between Junction A & B and the RBS in the junction B & C)

BBa_J70655
BBa_J70655 Version 1 (Component)
RFP optimized for expression in E. coli and M. florum

BBa_K861012
BBa_K861012 Version 1 (Component)
FadD and FadL with a RBS and a terminator

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_M45136
BBa_M45136 Version 1 (Component)
ChrR30, Chromate and Uranium Reductase

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_J92001
BBa_J92001 Version 1 (Component)
Lead Remover and Reporter Device

BBa_K1484005
BBa_K1484005 Version 1 (Component)
AmilCP chromoprotein in BBa and MoClo standard

BBa_K1113001
BBa_K1113001 Version 1 (Component)
pSB4K5 with a promoter and RBS

BBa_K1463771
BBa_K1463771 Version 1 (Component)
MotA and MotB under J23112 promoter

BBa_K546547
BBa_K546547 Version 1 (Component)
Constitutive (tetR repressible) LacI and RFP expression

RBS-TetR-T
BBa_K079007 Version 1 (Component)
TetR protein with terminator and RBS

BBa_M31909
BBa_M31909 Version 1 (Component)
Part of Gene 3 and the BamHI Restriction Site

BBa_K2097001
BBa_K2097001 Version 1 (Component)
Blue chromoprotein with strong promoter and RBS

BBa_K1685002
BBa_K1685002 Version 1 (Component)
aeBlue with LVA tag and double terminator