APP1
GenBank_CP047908_Region_1244167_1244926 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)

CnU6
CnU6_terminator Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans JEC21

URA5
GenBank_CM000046_Region_302227_303005 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans B-3501A

CnU6
GenBank_AE017348_Region_871991_872263 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans JEC21

ACT1
GenBank_CP003820_Region_1242235_1243067 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)

GAL7
GenBank_CP003831_Region_178967_179986 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubii H99

HYG
Hygromicin_Resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A strain H99O

URA5
GenBank_CM000046_Region_301512_302226 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans B-3501A

TEF1
GenBank_CP003831_Region_375181_376053 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubi H99

UGE2
GenBank_CP003831_Region_176453_177430 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubii H99

HOG1
GenBank_CP003830_Region_176667_176966 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubi H99

NEO (G418/Neomicin Resistance)
Neomicin_Resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A strain H99O

TEF1
GenBank_CP003831_Region_377755_378976 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubi H99

mCherry
mCherry Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

GPD
GenBank_AE017346_Region_940695_942557 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans JEC21

TRP1
GenBank_CP003828_Region_1023919_1024166 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubi H99

NAT (Nourseothricin Resistance/NrsR)
Nourseothricin_resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

GAL7
GenBank_AE017353_Region_190704_1911746 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans JEC21

URA5
GenBank_CM000046_Region_303006_303540 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans B-3501A

Cas9
Cas9 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubi H99

GAL1
GenBank_CP003831_Region_176110_177085 Version 1 (Unknown)
Organism: Cryptococcus neoformans var. grubii H99

GFP
GFP Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S

amdS
amdS Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A strain H99O

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

BBa_B0015
BBa_B0015 Version 1 (Component)
double terminator (B0010-B0012)

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

p(tetR)
BBa_R0040 Version 1 (Component)
TetR repressible promoter

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

LacI
BBa_R0010 Version 1 (Component)
promoter (lacI regulated)

BBa_I712074
BBa_I712074 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

pBAD
BBa_K206000 Version 1 (Component)
pBAD strong

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

BBa_M36010
BBa_M36010 Version 1 (Component)
Transcription Terminator (Strong)

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

lacI+pL
BBa_R0011 Version 1 (Component)
Promoter (lacI regulated, lambda pL hybrid)

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

T7 RBS
BBa_K1362090 Version 1 (Component)
strong T7 RBS