BBa_K195108
BBa_K195108 Version 1 (Component)
RBS+Lpp-OmpA-linker+ CCR5 gene+terminator

BBa_K431003
BBa_K431003 Version 1 (Component)
Pichia pastoris phosphatidylinositol 3-kinase gene, complete cds

BBa_K497003
BBa_K497003 Version 1 (Component)
Deinococcus radiodurans bacteriophytochrome gene with Ribosome Binding Site

BBa_K1686045
BBa_K1686045 Version 1 (Component)
crdA gene with codon optimisation for E. coli

BBa_K1807004
BBa_K1807004 Version 1 (Component)
Polyphosphate kinase gene from Accumulibacter phosphatis SK-12

BBa_K1395002
BBa_K1395002 Version 1 (Component)
sqr gene (sulfide quinone reductase) under constitutive promoter

BBa_K1686047
BBa_K1686047 Version 1 (Component)
crdC gene with codon optimisation for E. coli

BBa_K1395001
BBa_K1395001 Version 1 (Component)
nrfA gene (Nitrite reductase enzyme) under constitutive promoter

BBa_K1807012
BBa_K1807012 Version 1 (Component)
Accumulibacter phosphatis UW-1 str. Polyphosphate Kinase Gene

BBa_K1956010
BBa_K1956010 Version 1 (Component)
gRNA for Kanamycin resistant gene under lambda pL hybrid promoter

BBa_J31016
BBa_J31016 Version 1 (Component)
part produces the RNA construct crRNA-RBS-GFPLVA-tt that can only be translated in the presence of t

BBa_K2088006
BBa_K2088006 Version 1 (Component)
It encodes a kind of protein named 2Fe-2S ferredoxin, a 2Fe-2S iron-sulfur cluster binding domain. I

Devices from the iGEM 2016 interlab
iGEM_2016_interlab_collection Version 1 (Collection)
This is a collection of devices that were used in the 2016 iGEM interlab study

BBa_K1698001
BBa_K1698001 Version 1 (Component)
BaseHunter : Human Papiloma Virus (HPV) Detector Part - Can be activated to become detector for 55bp

BBa_K294208
BBa_K294208 Version 1 (Component)
luxC gene, acyl-CoA reductase LuxC from Vibrio fischeri ES114

BBa_J22146
BBa_J22146 Version 1 (Component)
The Lac Y and mRFP gene under the RecA(SOS) promoter.

BBa_K223002
BBa_K223002 Version 1 (Component)
RBS + SoxR- ribosome binding site and SoxR gene

BBa_M50013
BBa_M50013 Version 1 (Component)
Arsenic Sensor (arsR operon promoter and arsR gene)

P4Ag
BBa_K1889021 Version 1 (Component)
Constitutive Promoter Expressing PAL, 4CL and AtCCR1 gene

cry11Aa
BBa_K332011 Version 1 (Component)
Crystal protein gene (cry11Aa) from Bacillus thuringiensis subsp. Israelensis.

BBa_K1113411
BBa_K1113411 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome

BBa_K1189029
BBa_K1189029 Version 1 (Component)
TALE-A with a his tag linked to a K coil under the control of a LacI promoter

BBa_K1367004
BBa_K1367004 Version 1 (Component)
siRNA for LAR ( leucoanthocyanidin reductase) gene in Camelia sinensis

BBa_K549035
BBa_K549035 Version 1 (Component)
B. subtilis cue promoter with cueR + GFP reporter gene

BBa_K1923011
BBa_K1923011 Version 1 (Component)
Gal4BD-NLS-FLAG-EGFP-dCas9-NLS-Gal4AD encoding gene

BBa_K549033
BBa_K549033 Version 1 (Component)
B. subtilis ase promoter with aseR + lacZ' reporter gene

BBa_K549037
BBa_K549037 Version 1 (Component)
B. subtilis ase promoter with aseR + GFP reporter gene

BBa_M11062
BBa_M11062 Version 1 (Component)
pfaC gene for omega-3 polyunsaturated fatty acid synthase

BBa_K1187001
BBa_K1187001 Version 1 (Component)
Human insulin gene, optimized for expression in Pichia pastoris

BBa_K549032
BBa_K549032 Version 1 (Component)
B. subtilis ase promoter with aseR + GFP reporter gene

BBa_K549036
BBa_K549036 Version 1 (Component)
B. subtilis cue promoter with cueR + lacZ' reporter gene

BBa_K300980
BBa_K300980 Version 1 (Component)
BBa_K300001 construction intermediate (provided by Mr Gene)

nhaS
BBa_K1255000 Version 1 (Component)
nhaS gene that express NhaS, a putative sodium ion binding protein.

BBa_K1773026
BBa_K1773026 Version 1 (Component)
IPTG inducible cI repressor cloned with LuxI gene

BBa_J85001
BBa_J85001 Version 1 (Component)
inducible RFP, constitutive GFP, terminators after each gene

BBa_K2165002
BBa_K2165002 Version 1 (Component)
Violacein C gene with CUP1 promoter + ADH1 terminator

BBa_K2165003
BBa_K2165003 Version 1 (Component)
Violacein D gene with Gal1 promoter + ADH1 terminator

BBa_K804011
BBa_K804011 Version 1 (Component)
Constitutive EYFP generator - Arabinose inducible MetA coding gene

BBa_M1017
BBa_M1017 Version 1 (Component)
Silver Fusion Compatible PhaB gene with zif23-GCN4

BBa_M45102
BBa_M45102 Version 1 (Component)
Cobalt detection Biobrick. RFP made in presence of Cobalt. Note the receptor also works in the prese

BBa_K300983
BBa_K300983 Version 1 (Component)
BBa_K300000 construction intermediate (provided by Mr Gene)

BBa_K737000
BBa_K737000 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

YFP_SPfer
BBa_K809314 Version 1 (Component)
YFP + signal peptide of FER

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K112402
BBa_K112402 Version 1 (Component)
promoter for FabA gene - Membrane Damage and Ultrasound Senstitive

BBa_M1205
BBa_M1205 Version 1 (Component)
Complete Part for ROSE Regulated Kill Gene

BBa_M31748
BBa_M31748 Version 1 (Component)
Gene VII w/o Genes IX RBS or VIII Promoter

BBa_K374013
BBa_K374013 Version 1 (Component)
Lambda N-gene followed by BioBrick RFP BBa_E1010

BBa_K262002
BBa_K262002 Version 1 (Component)
Cinnamoyl-CoA reductase 2 Gene and Double Terminator

BBa_K1811777
BBa_K1811777 Version 1 (Component)
CUP1 copper resistance gene with RBS and promoter