BBa_K812001BBa_K812001 Version 1 (Component)Biobricked pSC2+ plasmid with pElastase promoter for frog use
BBa_K812012BBa_K812012 Version 1 (Component)OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin
BBa_K812013BBa_K812013 Version 1 (Component)GFP-AID OsTirI polysistronic system for auxin detection in tadpole
BBa_K812014BBa_K812014 Version 1 (Component)Auxin production device for expression in tadpole
CBDclosBBa_K863111 Version 1 (Component)Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)
BBa_K873000BBa_K873000 Version 1 (Component)heat shock 10kDa protein(hsp 10) groES coding resquence
BBa_K739004BBa_K739004 Version 1 (Component)Ax21 protein - signaling part
LipABBa_K836000 Version 1 (Component)lipA from B. cepacia (codon usage optimized for E. coli)
DGATBBa_K836001 Version 1 (Component)O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus)
DGATBBa_K836002 Version 1 (Component)O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus) as used
lipABBa_K836003 Version 1 (Component)lipA from B. cepacia (codon usage optimized for E. coli)
pLac ColE2BBa_K914001 Version 1 (Component)pLac Colicin E2 Immunity protein
BBa_K914002BBa_K914002 Version 1 (Component)repressilator RBS Colicin E2 Immunity protein
BBa_K883160BBa_K883160 Version 1 (Component)CCMV_Negative coat protein
BBa_K883151BBa_K883151 Version 1 (Component)CCMV coat protein gene 25AA N-terminal replaced with HIStag under IPTG inducable promotor
gpSBBa_K836004 Version 1 (Component)Lysis inhibitor from Enterobacteria phage lambda (codon usage optimized for R. opacus)
BBa_K157010BBa_K157010 Version 1 (Component)glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac
BBa_K836005BBa_K836005 Version 1 (Component)Lysozyme from Rhodococcus phage RER2 (codon usage optimized for R, opacus)
gpSBBa_K836006 Version 1 (Component)Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)
nitRBBa_K836007 Version 1 (Component)Nitrilase regulator from R. rhodochrous (codon usage optimized for R. opacus)
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
Cello E. Coli PartsEco1C1G1T1_collection Version 1 (Collection)These are the Cello parts for E. Coli circuits
linkerBBa_K157013 Version 1 (Component)15 aa flexible glycine-serine protein domain linker; Freiburg standard
rTT+pRBBa_K093003 Version 1 (Component)Convergent Promoter System: Forward Module: rTT+lambda pR
Q04510+RFPBBa_K131018 Version 1 (Component)Intermediate for the Response circuit
BBa_K101016BBa_K101016 Version 1 (Component)Dually repressed promoter with sites for TetR and P22MNT binding
BBa_K156009BBa_K156009 Version 1 (Component)OFP (orange fluorescent protein)
SBFP2BBa_K156010 Version 1 (Component)SBFP2 (strongly enhanced blue fluorescent protein)
BBa_K125810BBa_K125810 Version 1 (Component)slr2016 signal sequence + GFP fusion for secretion of GFP
OriTRBBa_J01003 Version 1 (Component)OriT-R (Origin of transfer for the R-plasmid nic region)
pBSEP1BBa_K090401 Version 1 (Component)Gram-positive Shuttle Vector for Episomal Expression
BBa_I0466BBa_I0466 Version 1 (Component)RhlR Protein Generator
BBa_K116001BBa_K116001 Version 1 (Component)nhaA promoter, that can be regulated by pH and nhaR protein.
BBa_K079051BBa_K079051 Version 1 (Component)LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator
BBa_K079052BBa_K079052 Version 1 (Component)LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato
BBa_K127013BBa_K127013 Version 1 (Component)gas vesicle protein A from cyanobacteria Anabaena
BBa_J36835BBa_J36835 Version 1 (Component)Lipoprotein signal peptide
BBa_J36836BBa_J36836 Version 1 (Component)Outer membrane protein A, aa 46-66
BBa_K116102BBa_K116102 Version 1 (Component)IPTG inducible membrane protein generator
BBa_K090403BBa_K090403 Version 1 (Component)Gram-positive Shuttle Vector for Chromosomal Integration
BBa_K129003BBa_K129003 Version 1 (Component)LamB-maltose transporter membrane protein