BBa_K086010
BBa_K086010 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter

BBa_K086015
BBa_K086015 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K1362109
BBa_K1362109 Version 1 (Component)
RBS + NpuDnaE N&C-Intein coexpression RFC[105] nonsplicing assembly construct (with His6)

CMV + GFP
BBa_K1852000 Version 1 (Component)
GFP + CMV Promoter used for expression in oocyte cells. This part was planned to be used as a report

BBa_J70655
BBa_J70655 Version 1 (Component)
RFP optimized for expression in E. coli and M. florum

BBa_K1400000
BBa_K1400000 Version 1 (Component)
PTRE(4)GX Dual input promoter. Activation at tetO binding sites, repression at gal4 sites.

BBa_K1400001
BBa_K1400001 Version 1 (Component)
PTRE(2)GX Dual input promoter. Activation at tetO binding sites, repression at gal4 sites.

BBa_K1039021
BBa_K1039021 Version 1 (Component)
Bxb1 integrase and Recombination Directionality Factor (RDF) Under ???Lock and Key??? Control with L

BBa_J44015
BBa_J44015 Version 1 (Component)
T7 promoter - RBS - RFP1 - hixC - RFP2

BBa_M11410
BBa_M11410 Version 1 (Component)
Type 2 promoter of sigE gene. Sigma factor regulates light and nitrogen responses, and has been obse

BBa_K1195011
BBa_K1195011 Version 1 (Component)
T7 Bacteriophage Minor Capsid Protein (Small Mutant 2)

BBa_K1195012
BBa_K1195012 Version 1 (Component)
T7 Bacteriophage Minor Capsid Protein (Large Mutant 2)

BBa_K1033204
BBa_K1033204 Version 1 (Component)
pSBLb4E15 E. coli and lactobacilli shuttle vector with erythromycin resistance

BBa_K2037011
BBa_K2037011 Version 1 (Component)
Laccase For in vitro expression

BBa_K145105
BBa_K145105 Version 1 (Component)
cI under T7 and P_R dual promotor

BBa_K1974013
BBa_K1974013 Version 1 (Component)
T7 Promoter+RBS+OAIP+linker+6X His-Tag

BBa_K145103
BBa_K145103 Version 1 (Component)
T7 with UmuD derived tag under constitutive promotor + RiboLock3d

BBa_K802003
BBa_K802003 Version 1 (Component)
Shuttle vector for <i> E. coli</i> and <i>B. subtilis</i>

BBa_K809710
BBa_K809710 Version 1 (Component)
GAL promoter + kozak + ZIM17 + T7 RNAP + ADH1 terminator

BBa_J13076
BBa_J13076 Version 1 (Component)
Monocistronic CFP/YFP expression cassette

BBa_K546547
BBa_K546547 Version 1 (Component)
Constitutive (tetR repressible) LacI and RFP expression

BBa_K1323019
BBa_K1323019 Version 1 (Component)
Hfq expression cassette under a xylose inducible promoter

BBa_K1438010
BBa_K1438010 Version 1 (Component)
Bacterial Iron Storage Expressor

BBa_K145112
BBa_K145112 Version 1 (Component)
cI under T7 and PR_R dual promotor

BBa_K812132
BBa_K812132 Version 1 (Component)
mCFP with kozak sequence for expression in Xenopus

BBa_K812133
BBa_K812133 Version 1 (Component)
sfGFP with kozak sequence for expression in Xenopus

BBa_K1497014
BBa_K1497014 Version 1 (Component)
Pelargonidin producing operon (low yield)- T7-B0034-F3H-B0034-DFR-B0034-ANS

BBa_K726009
BBa_K726009 Version 1 (Component)
T7 driven lac operated inducer for the rhl quorum-sensing system

BBa_K1974033
BBa_K1974033 Version 1 (Component)
T7 Promoter+RBS+Hv1a+GS linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1363200
BBa_K1363200 Version 1 (Component)
Anti-LPS factor(LALF) regulated by lacI

BBa_K2144011
BBa_K2144011 Version 1 (Component)
Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter

BBa_K812130
BBa_K812130 Version 1 (Component)
Citrine reporter with a Kozak sequence for expression in Xenopus

BBa_K1154006
BBa_K1154006 Version 1 (Component)
Mating pheromone-induced IGPD and constitutive LDH expression in yeast

BBa_K1650000
BBa_K1650000 Version 1 (Component)
Constitutive promoter expressing GFP (ILS 2015)

BBa_K2092004
BBa_K2092004 Version 1 (Component)
alcR (incl RBS), ethanol-activated transcription factor from A. nidulans

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.